{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T23:06:48Z","timestamp":1770764808003,"version":"3.50.0"},"reference-count":0,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2002,12,1]],"date-time":"2002-12-01T00:00:00Z","timestamp":1038700800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2002,12]]},"abstract":"\n The resistance phenotype of the clinical isolate of\n Escherichia coli<\/jats:italic>\n 1941 was characterized by high-level resistance to penicillins and to combinations amoxicillin-ticarcillin\/clavulanate and ampicillin\/sulbactam.\n This resistance was carried by the conjugative plasmid pEC1941 that encoded a\n \u03b2<\/jats:italic>\n -lactamase activity. The purified enzyme focused at pI 5.4 and was strongly inhibited\n in vitro<\/jats:italic>\n by clavulanic\n acid (IC\n 50<\/jats:sub>\n = 0.09\n \u03bc<\/jats:italic>\n M). Nucleotide sequence analysis revealed identity between the plasmid borne\n bla<\/jats:italic>\n TEM<\/jats:sub>\n gene of\n E. coli<\/jats:italic>\n 1941 and the\n bla<\/jats:italic>\n TEM-1B<\/jats:sub>\n gene, except for a single C-to-T substitution at position 32 in the promoter region leading to the overlapping promoters\n Pa<\/jats:italic>\n and\n Pb<\/jats:italic>\n . No alterations in the expression of outer membrane porins\n OmpC and OmpF have been detected. These findings show that the resistance of\n E. coli<\/jats:italic>\n 1941 to the combinations of\n \u03b2<\/jats:italic>\n -lactams with\n \u03b2<\/jats:italic>\n -lactamase inhibitors is related to high-level\n production of TEM-1 enzyme expressed from the strong promoters\n Pa<\/jats:italic>\n and\n Pb<\/jats:italic>\n .\n <\/jats:p>","DOI":"10.1089\/10766290260469525","type":"journal-article","created":{"date-parts":[[2003,1,16]],"date-time":"2003-01-16T20:22:20Z","timestamp":1042748540000},"page":"267-272","source":"Crossref","is-referenced-by-count":2,"title":["Molecular Characterization of Amoxicillin-Clavulanate Resistance in a Clinical Isolate of\n Escherichia coli<\/i>"],"prefix":"10.1177","volume":"8","author":[{"given":"Amel","family":"Bellaaj","sequence":"first","affiliation":[{"name":"Laboratoire de Biochimie et de Technobiologie, Facult\u00e9 des Sciences de Tunis, 2092 EL-Manar II, Tunis, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claude","family":"Bollet","sequence":"additional","affiliation":[{"name":"Enveloppe Bact\u00e9rienne, Antibiotiques et Colonisation CJF 96-06 INSERM, Facult\u00e9 de M\u00e9decine, 13385 Marseille Cedex 05, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nejib","family":"Alfeddy","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie et de Technobiologie, Facult\u00e9 des Sciences de Tunis, 2092 EL-Manar II, Tunis, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ferid","family":"Limam","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie et de Technobiologie, Facult\u00e9 des Sciences de Tunis, 2092 EL-Manar II, Tunis, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cherifa","family":"Belhadj","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie et de Technobiologie, Facult\u00e9 des Sciences de Tunis, 2092 EL-Manar II, Tunis, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne","family":"Regli","sequence":"additional","affiliation":[{"name":"Enveloppe Bact\u00e9rienne, Antibiotiques et Colonisation CJF 96-06 INSERM, Facult\u00e9 de M\u00e9decine, 13385 Marseille Cedex 05, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renaud","family":"Chollet","sequence":"additional","affiliation":[{"name":"Enveloppe Bact\u00e9rienne, Antibiotiques et Colonisation CJF 96-06 INSERM, Facult\u00e9 de M\u00e9decine, 13385 Marseille Cedex 05, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Omrane","family":"Belhadj","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie et de Technobiologie, Facult\u00e9 des Sciences de Tunis, 2092 EL-Manar II, Tunis, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kamel","family":"Ben-Mahrez","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie et de Technobiologie, Facult\u00e9 des Sciences de Tunis, 2092 EL-Manar II, Tunis, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2002,12]]},"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/10766290260469525","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/10766290260469525","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:14:06Z","timestamp":1770747246000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/10766290260469525"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,12]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2002,12]]}},"alternative-id":["10.1089\/10766290260469525"],"URL":"https:\/\/doi.org\/10.1089\/10766290260469525","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,12]]}}}