{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T15:24:09Z","timestamp":1780586649295,"version":"3.54.1"},"reference-count":0,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2002,6,1]],"date-time":"2002-06-01T00:00:00Z","timestamp":1022889600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2002,6]]},"abstract":"\n Multiresistant\n Salmonella enterica<\/jats:italic>\n subspecies\n enterica<\/jats:italic>\n serovar Typhimurium definitive type 104 (\n S.<\/jats:italic>\n Typhimurium DT104 or DT104) bacteria are important pathogens in animals and humans.\n DT104 isolates are often called pentaresistant strains that spread clonally. The purpose of this study was to determine phenotypic, genotypic, and epidemiologic characteristics of 175\n S.<\/jats:italic>\n Typhimurium\n DT104 strains isolated from food-producing animals in Canada. More than 90% of the isolates were resistant to ampicillin (Amp), chloramphenicol (Chl), florfenicol (Flo), sulfisoxazole (Sul), and tetracycline\n (Tet), 53% of the isolates were additionally resistant to spectinomycin (Spc) and streptomycin (Str), and 28% to kanamycin (Kan) and neomycin (Neo). Sixty-one percent of the strains harbored a single 60-MDa\n plasmid, 21% contained 60- and 2.0-MDa plasmids, and 4% had 60, 4.6- and 2.0-MDa plasmids. Resistance to Kan and Neo was encoded by the aminoglycoside\n aphA-1<\/jats:italic>\n gene on 2.0-MDa plasmids, whereas resistance\n to trimethoprim (Tmp) and Sul was encoded by the\n dhfrIb<\/jats:italic>\n gene on 4.6-MDa plasmids. Polymerase chain reactions (PCR) showed the presence of integrons with the\n ant<\/jats:italic>\n (3\u2033)-\n Ia<\/jats:italic>\n aminoglycoside\n adenyltransferase and the\n bla<\/jats:italic>\n PSE-1<\/jats:sub>\n \u03b2<\/jats:italic>\n -lactamase gene cassettes, and the presence of the\n flo<\/jats:italic>\n St<\/jats:sub>\n gene in all but one strain resistant to Spc and Str, Amp, and\n Chl and Flo, respectively. DT104 isolates from cattle at six feedlots represented a separate clone; they were sensitive to Str and Spc and lacked the\n ant<\/jats:italic>\n (3\u2033)-\n Ia<\/jats:italic>\n gene. Pulsed-field gel\n electrophoresis (PFGE) using\n Bln<\/jats:italic>\n I,\n Spe<\/jats:italic>\n I, and\n Xba<\/jats:italic>\n I resulted in 15, 12, and 8 PFGE patterns, respectively. In summary, we observed considerable diversity in phenotypic, genotypic,\n and epidemiological characteristics among the DT104 isolates.\n <\/jats:p>","DOI":"10.1089\/107662902760190653","type":"journal-article","created":{"date-parts":[[2002,9,30]],"date-time":"2002-09-30T13:59:54Z","timestamp":1033394394000},"page":"107-122","source":"Crossref","is-referenced-by-count":50,"title":["Diversity in Antimicrobial Resistance and Other Characteristics Among\n Salmonella<\/i>\n Typhimurium DT104 Isolates"],"prefix":"10.1177","volume":"8","author":[{"given":"C.","family":"Poppe","sequence":"first","affiliation":[{"name":"Health Canada, Population and Public Health Branch, Laboratory for Foodborne Zoonoses, OI\u00c9 Reference Laboratory for Salmonellosis, Guelph, Ontario N1G 3W4, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Ziebell","sequence":"additional","affiliation":[{"name":"Health Canada, Population and Public Health Branch, Laboratory for Foodborne Zoonoses, OI\u00c9 Reference Laboratory for Salmonellosis, Guelph, Ontario N1G 3W4, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Martin","sequence":"additional","affiliation":[{"name":"Health Canada, Population and Public Health Branch, Laboratory for Foodborne Zoonoses, OI\u00c9 Reference Laboratory for Salmonellosis, Guelph, Ontario N1G 3W4, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Allen","sequence":"additional","affiliation":[{"name":"Health Canada, Population and Public Health Branch, Laboratory for Foodborne Zoonoses, OI\u00c9 Reference Laboratory for Salmonellosis, Guelph, Ontario N1G 3W4, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2002,6]]},"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/107662902760190653","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/107662902760190653","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:14:02Z","timestamp":1770747242000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/107662902760190653"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,6]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2002,6]]}},"alternative-id":["10.1089\/107662902760190653"],"URL":"https:\/\/doi.org\/10.1089\/107662902760190653","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,6]]}}}