{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T22:41:28Z","timestamp":1770763288185,"version":"3.50.0"},"reference-count":0,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2002,6,1]],"date-time":"2002-06-01T00:00:00Z","timestamp":1022889600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2002,6]]},"abstract":"\n Macrolide-resistance genes were investigated in 103 macrolide-resistant strains of\n Streptococcus pyogenes<\/jats:italic>\n , isolated from children with pharyngotonsillitis. The presence of\n mef<\/jats:italic>\n (A),\n erm<\/jats:italic>\n (B),\n and\n erm<\/jats:italic>\n (TR) genes was detected by PCR.\n mef<\/jats:italic>\n (A) was found in 48 out of 103 (46.6%) strains, whereas\n erm<\/jats:italic>\n (B) was detected in 43 isolates (41.7%). All\n mef<\/jats:italic>\n (A) strains showed a typical\n M phenotype (resistance to 14- and 15-membered macrolides, and sensitivity to lincosamides and streptogramin B), whereas\n erm<\/jats:italic>\n (B) strains had the MLS\n B<\/jats:sub>\n phenotype (resistance to macrolides,\n lincosamides, and streptogramin B antibiotics).\n erm<\/jats:italic>\n (TR) was found in 10 strains, always together with other resistance genes. In seven cases\n erm<\/jats:italic>\n (TR) was associated with\n erm<\/jats:italic>\n (B), and\n three cases with\n mef<\/jats:italic>\n (A). In two isolates with the M phenotype (1.9%), it was not possible to detect the presence of any of the three macrolide resistance genes tested. Inducible resistance to macrolides\n was shown for 24 out of the 53 MLS\n B<\/jats:sub>\n strains. Analysis of macrorestriction fragment patterns by pulsed-field gel electrophoresis showed that erythromycin-resistant\n S. pyogenes<\/jats:italic>\n are polyclonal,\n however each phenotype, MLS\n B<\/jats:sub>\n and M, formed essentially homogeneous groups.\n <\/jats:p>","DOI":"10.1089\/107662902760190671","type":"journal-article","created":{"date-parts":[[2002,9,30]],"date-time":"2002-09-30T13:59:54Z","timestamp":1033394394000},"page":"129-132","source":"Crossref","is-referenced-by-count":20,"title":["Macrolide-Resistance Genes in Clinical Isolates of\n Streptococcus pyogenes<\/i>"],"prefix":"10.1177","volume":"8","author":[{"given":"Carmela","family":"Cascone","sequence":"first","affiliation":[{"name":"Department of Microbiological Sciences, University of Catania, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Santagati","sequence":"additional","affiliation":[{"name":"Department of Microbiological Sciences, University of Catania, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvana","family":"Noviello","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, University of Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco","family":"Iannelli","sequence":"additional","affiliation":[{"name":"LA.M.M.B. (Laboratory of Molecular Microbiology and Biotechnology), Department of Molecular Biology, University of Siena, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvano","family":"Esposito","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, University of Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gianni","family":"Pozzi","sequence":"additional","affiliation":[{"name":"LA.M.M.B. (Laboratory of Molecular Microbiology and Biotechnology), Department of Molecular Biology, University of Siena, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefania","family":"Stefani","sequence":"additional","affiliation":[{"name":"Department of Microbiological Sciences, University of Catania, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2002,6]]},"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/107662902760190671","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/107662902760190671","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:14:03Z","timestamp":1770747243000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/107662902760190671"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,6]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2002,6]]}},"alternative-id":["10.1089\/107662902760190671"],"URL":"https:\/\/doi.org\/10.1089\/107662902760190671","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,6]]}}}