{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T19:24:06Z","timestamp":1783538646499,"version":"3.55.0"},"reference-count":0,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2002,9,1]],"date-time":"2002-09-01T00:00:00Z","timestamp":1030838400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2002,9]]},"abstract":"<jats:p>\n                    An\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    CM2555 strain, sensitive to chloramphenicol when expressing the\n                    <jats:italic toggle=\"yes\">cat<\/jats:italic>\n                    gene and producing active chloramphenicol acetyltransferase (CAT), was described recently. It was proposed\n         that this sensitivity is due to decreased levels of acetyl coenzyme A (Acetyl CoA) in\n                    <jats:italic toggle=\"yes\">cat<\/jats:italic>\n                    -expressing CM2555 cells in the presence of chloramphenicol. CAT catalyzes transfer of the acetyl moiety from\n         Acetyl CoA to a chloramphenicol molecule. Thus, a very efficient acetylation of chloramphenicol may cause deprivation of Acetyl CoA and cell death. A specific mutation causing the chloramphenicol sensitivity\n         phenotype of CM2555 was not reported to date. Therefore, we aimed to identify a genetic defect causing this phenotype. Here, we found that overexpression of the\n                    <jats:italic toggle=\"yes\">acrEF<\/jats:italic>\n                    genes, encoding a transmembrane\n         pump, or the\n                    <jats:italic toggle=\"yes\">acrE<\/jats:italic>\n                    gene alone, results in restoration of chloramphenicol-resistance of\n                    <jats:italic toggle=\"yes\">cat<\/jats:italic>\n                    -expressing CM2555 strain. Although no mutation exists in the CM2555\n                    <jats:italic toggle=\"yes\">acrE<\/jats:italic>\n                    locus, a nonsense\n         mutation in the 67th codon of the\n                    <jats:italic toggle=\"yes\">acrA<\/jats:italic>\n                    gene, which encodes a component of another transmembrane pump, has been found. Although introduction of the\n                    <jats:italic toggle=\"yes\">\u0394acrAB<\/jats:italic>\n                    allele into CM732, a parental\n         strain of CM2555, and into some other commonly used\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    strains led to their chloramphenicol sensitivity in the presence of CAT, the same genetic manipulation did not result in such a phenotype\n         in other genetic backgrounds, including \"wild-type\"\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    MG1655. These results suggest that the\n                    <jats:italic toggle=\"yes\">acrA<\/jats:italic>\n                    dysfunction is one of more mutations responsible for chloramphenicol sensitivity of\n                    <jats:italic toggle=\"yes\">cat<\/jats:italic>\n                    -expressing CM2555 strain.\n                  <\/jats:p>","DOI":"10.1089\/107662902760326887","type":"journal-article","created":{"date-parts":[[2002,9,25]],"date-time":"2002-09-25T14:37:28Z","timestamp":1032964648000},"page":"179-185","source":"Crossref","is-referenced-by-count":4,"title":["Inactivation of the\n                    <i>acrA<\/i>\n                    Gene Is Partially Responsible for Chloramphenicol Sensitivity of\n                    <i>Escherichia coli<\/i>\n                    CM2555 Strain Expressing the Chloramphenicol Acetyltransferase Gene"],"prefix":"10.1177","volume":"8","author":[{"given":"Joanna","family":"Potrykus","sequence":"first","affiliation":[{"name":"Department of Molecular Biology, University of Gda\u0144sk, K\u0142adki 24, 80-822 Gda\u0144sk, Poland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylwia","family":"Bara\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, University of Gda\u0144sk, K\u0142adki 24, 80-822 Gda\u0144sk, Poland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grzegorz","family":"Wegrzyn","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, University of Gda\u0144sk, K\u0142adki 24, 80-822 Gda\u0144sk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2002,9]]},"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/107662902760326887","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/107662902760326887","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:14:04Z","timestamp":1770747244000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/107662902760326887"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,9]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2002,9]]}},"alternative-id":["10.1089\/107662902760326887"],"URL":"https:\/\/doi.org\/10.1089\/107662902760326887","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,9]]}}}