{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T22:03:02Z","timestamp":1791237782744,"version":"4.1.0"},"reference-count":0,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2002,9,1]],"date-time":"2002-09-01T00:00:00Z","timestamp":1030838400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2002,9]]},"abstract":"\n Stenotrophomonas maltophilia<\/jats:italic>\n is a nosocomial pathogen with an intrinsic broad-spectrum resistance to\n \u03b2<\/jats:italic>\n -lactam compounds and other antibacterial agents. It produces two chromosomal\n \u03b2<\/jats:italic>\n -lactamases:\n a clavulanic acid-sensitive class A (L2) and a tetrameric carbapenemase (L1 or BlaS). We screened 40\n S. maltophilia<\/jats:italic>\n multidrug-resistant clinical isolates recovered between 1995 and 1998 in the Varese\n Hospital (Italy) for the presence of the metallo-\n \u03b2<\/jats:italic>\n -lactamase. The isolates were investigated by phenotypic profiling (enzymatic activity and antibiotic resistance pattern) and molecular methods\n such as PCR and pulsed-field gel electrophoresis (PFGE) to reveal intraspecies diversity. For the tested\n S. maltophilia<\/jats:italic>\n strains, we showed that the\n \u03b2<\/jats:italic>\n -lactamase production could be induced\n by the presence of imipenem (50\n \u03bc<\/jats:italic>\n g\/ml) in the culture media. Addition of 1 mM dipicolinic acid completely inhibited the hydrolysis of imipenem but decreased that nitrocefin in a strain-dependent\n manner. Full activity of crude extract towards imipenem could be restored by addition of 1 mM ZnCl\n 2<\/jats:sub>\n . Finally, the gene encoding the carbapenem-hydrolyzing\n \u03b2<\/jats:italic>\n -lactamase from\n S. maltophilia<\/jats:italic>\n ULA-511 and 39\/95, a clinical strain, were isolated and sequenced. These two strains have a different profile of multidrug resistance. The two metallo-\n \u03b2<\/jats:italic>\n -lactamases were found to be isologous.\n The difference of sensitivity of these two strains was associated to the level of production of the metallo-\n \u03b2<\/jats:italic>\n -lactamase.\n <\/jats:p>","DOI":"10.1089\/107662902760326904","type":"journal-article","created":{"date-parts":[[2002,9,25]],"date-time":"2002-09-25T14:37:28Z","timestamp":1032964648000},"page":"193-200","source":"Crossref","is-referenced-by-count":26,"title":["Clonal Diversity and Metallo-\n \u03b2<\/i>\n -Lactamase Production in Clinical Isolates of\n Stenotrophomonas maltophilia<\/i>"],"prefix":"10.1177","volume":"8","author":[{"given":"Paola Sandra","family":"Mercuri","sequence":"first","affiliation":[{"name":"Centre d'Ing\u00e9nierie des Prot\u00e9ines, Universit\u00e9 de Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshikazu","family":"Ishii","sequence":"additional","affiliation":[{"name":"Centre d'Ing\u00e9nierie des Prot\u00e9ines, Universit\u00e9 de Li\u00e8ge, Belgium and Department of Microbiology, Toho University, School of Medicine, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Toho University, School of Medicine, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gian Maria","family":"Rossolini","sequence":"additional","affiliation":[{"name":"Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Universit\u00e0 di Siena, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Luzzaro","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Ospedale di Circolo e Fondazione Macchi, Varese, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianfranco","family":"Amicosante","sequence":"additional","affiliation":[{"name":"Department S.T.B., Universit\u00e0 dell'Aquila, Aquila, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola","family":"Franceschini","sequence":"additional","affiliation":[{"name":"Department S.T.B., Universit\u00e0 dell'Aquila, Aquila, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Marie","family":"Frere","sequence":"additional","affiliation":[{"name":"Centre d'Ing\u00e9nierie des Prot\u00e9ines, Universit\u00e9 de Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moreno","family":"Galleni","sequence":"additional","affiliation":[{"name":"Centre d'Ing\u00e9nierie des Prot\u00e9ines, Universit\u00e9 de Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2002,9]]},"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/107662902760326904","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/107662902760326904","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:14:04Z","timestamp":1770747244000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/107662902760326904"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,9]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2002,9]]}},"alternative-id":["10.1089\/107662902760326904"],"URL":"https:\/\/doi.org\/10.1089\/107662902760326904","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,9]]}}}