{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T02:40:03Z","timestamp":1773283203357,"version":"3.50.1"},"reference-count":0,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2003,12,1]],"date-time":"2003-12-01T00:00:00Z","timestamp":1070236800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2003,12]]},"abstract":"<jats:p>\n                    Penicillin-binding proteins (PBPs) mediate susceptibility to \u03b2-lactam antibiotics. PBP 4, although not essential for survival, has been associated with low-level resistance to \u03b2-lactam antibiotics.\n         To determine its contribution to survival of\n                    <jats:italic toggle=\"yes\">Staphylococcus aureus<\/jats:italic>\n                    cells exposed to \u03b2-lactams, the PBP 4 gene (\n                    <jats:italic toggle=\"yes\">pbp4<\/jats:italic>\n                    ) was disrupted and then complemented in the methicillin-susceptible\n         strain RN4220 and the homogeneous methicillin-resistant strain COL. Depending on the antibiotic tested, the presence or absence of an intact\n                    <jats:italic toggle=\"yes\">pbp4<\/jats:italic>\n                    has no effect or only a modest effect on growth measured\n         by population analysis. These data indicate that PBP 4 is a relatively unimportant target of \u03b2-lactams not only in methicillin-susceptible but also methicillin-resistant\n                    <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1089\/107662903322762752","type":"journal-article","created":{"date-parts":[[2004,2,9]],"date-time":"2004-02-09T18:52:42Z","timestamp":1076352762000},"page":"329-336","source":"Crossref","is-referenced-by-count":20,"title":["Effect of Disruption of\n                    <i>Staphylococcus aureus<\/i>\n                    PBP4 Gene on Resistance to \u03b2-Lactam Antibiotics"],"prefix":"10.1177","volume":"9","author":[{"given":"Yuki","family":"Katayama","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, San Francisco General Hospital, Department of Medicine, University of California San Francisco, San Francisco, CA 94110"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong-Zhong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, San Francisco General Hospital, Department of Medicine, University of California San Francisco, San Francisco, CA 94110"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henry F.","family":"Chambers","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, San Francisco General Hospital, Department of Medicine, University of California San Francisco, San Francisco, CA 94110"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2003,12]]},"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/107662903322762752","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/107662903322762752","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:14:20Z","timestamp":1770747260000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/107662903322762752"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,12]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2003,12]]}},"alternative-id":["10.1089\/107662903322762752"],"URL":"https:\/\/doi.org\/10.1089\/107662903322762752","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,12]]}}}