{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T02:15:15Z","timestamp":1770776115850,"version":"3.50.0"},"reference-count":0,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2003,12,1]],"date-time":"2003-12-01T00:00:00Z","timestamp":1070236800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2003,12]]},"abstract":"\n Plasmid-mediated resistance to tetracycline in Neisseria gonorrhoeae (TRNG) isolates is caused by the acquisition of a 25.2-MDa conjugative,\n tetM<\/jats:italic>\n -containing plasmid (TetM plasmid). The presence of\n the TetM plasmid is the leading cause of gonococcal resistance to tetracycline in most countries. Between 1986 and 1997, 6,306 TRNG isolates were isolated in different Canadian provincial laboratories and\n subsequently submitted to the national laboratory for further strain characterization. Because nonculture-based identification of\n N. gonorrhoeae<\/jats:italic>\n was more widely used after 1995, this snapshot of\n the molecular epidemiology of TRNG in Canada, which is only possible if bacteria are cultured, represents a comprehensive data baseline that may no longer be achievable. Temporal trends indicate that TRNG\n isolations peaked in 1994 (18.9% of isolates tested). Antimicrobial susceptibilities (MIC) to tetracycline and penicillin were determined for 4,064 TRNG isolated between 1986 and 1994. The MICs of TRNG\n isolates ranged from 16\u03bcg\/ml to 32\u03bcg\/ml of tetracycline, although one isolate had an MIC of 8\u03bcg\/ml and the MICs of four isolates were 2\u03bcg\/ml. Penicillinase-producing TRNG (\n i.e.<\/jats:italic>\n , PP\/TRNG)\n comprised 34.1% of all TRNG (\n n<\/jats:italic>\n = 1,386) and 52 TRNG isolates exhibited chromosomal resistance to penicillin. Most of the PP\/TRNG (94.1%) carried Africa type (3.2 MDa)\n \u03b2<\/jats:italic>\n -lactamase-producing\n plasmids; only 76 (5.5%) PP\/TRNG carried Asia type (4.4 MDa) penicillinase-producing plasmids and three isolates carried Toronto type (3.05 MDa) plasmids. TRNG isolates were also retrospectively typed by\n auxotype (A), serovar (S), and plasmid (P) content analysis. Eleven auxotype\/serovar (A\/S) groups comprised the majority (93%) of 4,064 typed TRNG isolates with A\/S classes NR\/IB-2, NR\/IB-3, and NR\/IB-1\n accounting for 75.1% of the strains characterized. Classification of 670 TRNG for\n tetM<\/jats:italic>\n type demonstrated that the Dutch (\n n<\/jats:italic>\n = 531) type TetM plasmids predominated over the American (\n n<\/jats:italic>\n = 139) type TetM plasmids.\n <\/jats:p>","DOI":"10.1089\/107662903322762789","type":"journal-article","created":{"date-parts":[[2004,2,9]],"date-time":"2004-02-09T18:52:42Z","timestamp":1076352762000},"page":"353-360","source":"Crossref","is-referenced-by-count":9,"title":["Molecular Epidemiology of\n Neisseria gonorrheae<\/i>\n Isolates with Plasmid-Mediated Tetracycline Resistance in Canada: Temporal and Geographical Trends (1986-1997)"],"prefix":"10.1177","volume":"9","author":[{"given":"Valerie","family":"Greco","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Canada K1H 8M5"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lai-King","family":"Ng","sequence":"additional","affiliation":[{"name":"National Laboratory for Microbiology, Winnipeg, Manitoba, Canada K1A 0L2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Catana","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Canada K1H 8M5"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Canada K1H 8M5"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo-Anne R.","family":"Dillon","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Microbiology and Immunology and Centre for Research in Biopharmaceuticals and Biotechnology, University of Ottawa, Ottawa, Canada K1H 8M5"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2004,7,8]]},"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/107662903322762789","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/107662903322762789","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:14:21Z","timestamp":1770747261000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/107662903322762789"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,12]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2003,12]]}},"alternative-id":["10.1089\/107662903322762789"],"URL":"https:\/\/doi.org\/10.1089\/107662903322762789","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,12]]}}}