{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:23:43Z","timestamp":1791307423769,"version":"4.3.1"},"reference-count":0,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2004,6,1]],"date-time":"2004-06-01T00:00:00Z","timestamp":1086048000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2004,6]]},"abstract":"<jats:p>\n                    Previous genotypic investigations of extended-spectrum \u03b2-lactamase (ESBL)-producing\n                    <jats:italic toggle=\"yes\">Klebsiella pneumoniae<\/jats:italic>\n                    recovered in a Tunisian neonatal ward revealed the spread of two epidemic strains and a high number of genetically unrelated isolates. The aim of the present study was to \ndetermine the role of the dissemination of self-transferrable plasmids harboring\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    genes in the outbreaks experienced by the ward. The 49 previously identified clinical isolates of ESBL-producing\n                    <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                    were examined for relationships between their enzymes and plasmids. \nAnalysis of crude extracts by isoelectric focusing showed four \u03b2-lactamase-activities at pI 8.2, 7.6, 6, and 5.4. Clinical isolates contained large plasmids that could be transferred by conjugation and transformation conferring resistance to expanded-spectrum cephalosporins. DNA amplification \nand sequencing were performed to confirm the identities of transferred \u03b2-lactamases. Nucleotide sequence analysis of SHV-specific PCR products from six isolates identified two\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>SHV<\/jats:sub>\n                    genes corresponding to SHV derived ESBLs, SHV-12 and SHV-2a.\n                    <jats:italic toggle=\"yes\">Pst<\/jats:italic>\n                    I digestion of plasmid \nDNA from transformants revealed six restriction patterns. The occurrence of the prevalent plasmid pattern in both epidemic strains and unrelated isolates indicated that diffusion and endemic persistence of the\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>SHV-ESBL<\/jats:sub>\n                    genes in the ward were due to concomitant spread of \nepidemic strains and plasmid dissemination among unrelated strains.\n                  <\/jats:p>","DOI":"10.1089\/1076629041310118","type":"journal-article","created":{"date-parts":[[2004,7,1]],"date-time":"2004-07-01T19:05:46Z","timestamp":1088708746000},"page":"132-138","source":"Crossref","is-referenced-by-count":27,"title":["Involvement of SHV-12 and SHV-2a Encoding Plasmids in Outbreaks of Extended-Spectrum \u03b2-Lactamase-Producing\n                    <i>Klebsiella pneumoniae<\/i>\n                    in a Tunisian Neonatal Ward"],"prefix":"10.1177","volume":"10","author":[{"given":"Thouraya","family":"Ben-Hamouda","sequence":"first","affiliation":[{"name":"Laboratoire de Biochimie et de Biotechnologie, Facult\u00e9 des Sciences de Tunis, Universit\u00e9 Tunis El Manar, Tunis, Tunisia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Foulon","sequence":"additional","affiliation":[{"name":"Biochimie des Signaux R\u00e9gulateurs Cellulaires et Mol\u00e9culaires, Universit\u00e9 Pierre et Marie Curie\u2014Centre National de la Recherche Scientifique, F-75006 Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamel","family":"Ben-Mahrez","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie et de Biotechnologie, Facult\u00e9 des Sciences de Tunis, Universit\u00e9 Tunis El Manar, Tunis, Tunisia."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2004,6]]},"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/1076629041310118","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/1076629041310118","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T12:32:10Z","timestamp":1777638730000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/1076629041310118"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,6]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2004,6]]}},"alternative-id":["10.1089\/1076629041310118"],"URL":"https:\/\/doi.org\/10.1089\/1076629041310118","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,6]]}}}