{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T09:57:05Z","timestamp":1774691825188,"version":"3.50.1"},"reference-count":0,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2000,3,1]],"date-time":"2000-03-01T00:00:00Z","timestamp":951868800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial & Comparative Genomics"],"published-print":{"date-parts":[[2000,3,1]]},"abstract":"\n Francisella tularensis<\/jats:italic>\n is the etiological agent of tularemia, a serious disease in several Northern hemisphere countries. The organism has fastidious growth requirements and is very poorly understood\n at the genetic and molecular levels. Given the lack of data on this organism, we undertook the sample sequencing of its genome. A random library of DNA fragments from a highly virulent strain (Schu 4) of\n F. tularensis<\/jats:italic>\n was constructed and the nucleotide sequences of 13,904 cloned fragments were determined and assembled into 353 contigs. A total of 1.83 Mb of nucleotide sequence was obtained that had\n a G + C content of 33.2%. Genes located on plasmids pOM1 and pNFL10, which had been previously isolated from low virulence strains of\n F. tularensis<\/jats:italic>\n , were absent but all of the other known\n F. tularensis<\/jats:italic>\n genes were represented in the assembled data.\n F. tularensis<\/jats:italic>\n Schu4 was able to grow in the absence of aromatic amino acids and orthologues of genes which could encode enzymes in the shikimate pathway\n in other bacteria were identified in the assembled data. Genes that could encode all of the enzymes in the purine biosynthetic and most of the enzymes in the purine salvage pathways were also identified.\n This data will be used to develop defined rationally attenuated mutants of\n F. tularensis<\/jats:italic>\n , which could be used as replacements for the existing genetically undefined live vaccine strain.\n <\/jats:p>","DOI":"10.1089\/10906590050145249","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T18:05:48Z","timestamp":1027706748000},"page":"25-39","source":"Crossref","is-referenced-by-count":42,"title":["Sequencing of the\n Francisella tularensis<\/i>\n Strain Schu 4 Genome Reveals the Shikimate and Purine Metabolic Pathways, Targets for the Construction of a Rationally Attenuated Auxotrophic Vaccine"],"prefix":"10.1177","volume":"5","author":[{"given":"Jan","family":"Karlsson","sequence":"first","affiliation":[{"name":"National Defence Research Establishment, Ume\u00e5, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard G.","family":"Prior","sequence":"additional","affiliation":[{"name":"Defence Evaluation and Research Agency, CBD Porton Down, Salisbury, Wilts, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kerstin","family":"Williams","sequence":"additional","affiliation":[{"name":"Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luther","family":"Lindler","sequence":"additional","affiliation":[{"name":"Department of Bacterial Diseases, Walter Reed Army Institute of Research, Washington, D.C."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katherine A.","family":"Brown","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Imperial College of Science Technology and Medicine, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicola","family":"Chatwell","sequence":"additional","affiliation":[{"name":"Defence Evaluation and Research Agency, CBD Porton Down, Salisbury, Wilts, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karin","family":"Hjalmarsson","sequence":"additional","affiliation":[{"name":"National Defence Research Establishment, Ume\u00e5, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nick","family":"Loman","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, St. Bartholomews and the Royal London School of Medicine and Dentistry, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kerri A.","family":"Mack","sequence":"additional","affiliation":[{"name":"Defence Evaluation and Research Agency, CBD Porton Down, Salisbury, Wilts, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Pallen","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunobiology, The Queen's University Belfast, Belfast, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Popek","sequence":"additional","affiliation":[{"name":"Department of Bacterial Diseases, Walter Reed Army Institute of Research, Washington, D.C."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gunnar","family":"Sandstr\u00f6m","sequence":"additional","affiliation":[{"name":"National Defence Research Establishment, Ume\u00e5, Sweden and Department of Clinical Microbiology, Infectious Diseases, Ume\u00e5 University, Ume\u00e5, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anders","family":"Sj\u00f6stedt","sequence":"additional","affiliation":[{"name":"National Defence Research Establishment, Ume\u00e5, Sweden and Department of Clinical Microbiology, Clinical Bacteriology, Ume\u00e5 University, Ume\u00e5, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Svensson","sequence":"additional","affiliation":[{"name":"National Defence Research Establishment, Ume\u00e5, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivica","family":"Tamas","sequence":"additional","affiliation":[{"name":"Department of Molecular Evolution, University of Uppsala, Uppsala, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siv G. E.","family":"Andersson","sequence":"additional","affiliation":[{"name":"Department of Molecular Evolution, University of Uppsala, Uppsala, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brendan W.","family":"Wren","sequence":"additional","affiliation":[{"name":"Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petra C. F.","family":"Oyston","sequence":"additional","affiliation":[{"name":"Defence Evaluation and Research Agency, CBD Porton Down, Salisbury, Wilts, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard W.","family":"Titball","sequence":"additional","affiliation":[{"name":"Defence Evaluation and Research Agency, CBD Porton Down, Salisbury, Wilts, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2000,3,1]]},"container-title":["Microbial & Comparative Genomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/10906590050145249","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/10906590050145249","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T09:43:47Z","timestamp":1774691027000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/10906590050145249"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,3,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2000,3,1]]}},"alternative-id":["10.1089\/10906590050145249"],"URL":"https:\/\/doi.org\/10.1089\/10906590050145249","relation":{},"ISSN":["1090-6592"],"issn-type":[{"value":"1090-6592","type":"print"}],"subject":[],"published":{"date-parts":[[2000,3,1]]}}}