{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T18:33:29Z","timestamp":1771266809695,"version":"3.50.1"},"reference-count":0,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2002,10,1]],"date-time":"2002-10-01T00:00:00Z","timestamp":1033430400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["OMICS: A Journal of Integrative Biology"],"published-print":{"date-parts":[[2002,10]]},"abstract":"<jats:p>\n                    We describe our strategy for selecting targets for protein structure determination in context of structural genomics of a single genome. In the course of target selection, we have studied two the smallest\n         microbial genomes,\n                    <jats:italic toggle=\"yes\">Mycoplasma genitalium<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">Mycoplasma pneumoniae<\/jats:italic>\n                    . To our surprise, we found that only 71\n                    <jats:italic toggle=\"yes\">Mycoplasma<\/jats:italic>\n                    genes or their orthologues can be considered as easy targets for\n         high-throughput structural studies - far fewer than expected. We discuss the methods and criteria used for target selection and the reasons explaining rarity of easy targets. First, despite the common opinion\n         that protein folds can be predicted for only 30-50% of genes, the number of \"truly unknown\" structures is less than one-third. Second, due to the different codon usage, two thirds of\n                    <jats:italic toggle=\"yes\">Mycoplasma<\/jats:italic>\n                    proteins\n         cannot be directly expressed in\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    in high-throughput manner and require substitution by their homologues from other organisms. Third, membrane or large multi-domain proteins are difficult targets\n         because of solubility and size issues and often require identification and structure determination of protein domains. Finally, we propose different approaches to address the difficult targets.\n                  <\/jats:p>","DOI":"10.1089\/153623102321112773","type":"journal-article","created":{"date-parts":[[2003,3,5]],"date-time":"2003-03-05T21:06:03Z","timestamp":1046898363000},"page":"349-362","source":"Crossref","is-referenced-by-count":3,"title":["Target Selection for Structural Genomics: A Single Genome Approach"],"prefix":"10.1177","volume":"6","author":[{"given":"Igor V.","family":"Grigoriev","sequence":"first","affiliation":[{"name":"Department of Chemistry and E.O. Lawrence Berkeley National Laboratory, University of California, Berkeley, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"In-Geol","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Chemistry and E.O. Lawrence Berkeley National Laboratory, University of California, Berkeley, California"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2002,10]]},"container-title":["OMICS: A Journal of Integrative Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/153623102321112773","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/153623102321112773","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T17:39:40Z","timestamp":1771263580000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/153623102321112773"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,10]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2002,10]]}},"alternative-id":["10.1089\/153623102321112773"],"URL":"https:\/\/doi.org\/10.1089\/153623102321112773","relation":{},"ISSN":["1536-2310","1557-8100"],"issn-type":[{"value":"1536-2310","type":"print"},{"value":"1557-8100","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,10]]}}}