{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:19:51Z","timestamp":1788481191908,"version":"build-2803163510"},"reference-count":0,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2002,7,1]],"date-time":"2002-07-01T00:00:00Z","timestamp":1025481600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["OMICS: A Journal of Integrative Biology"],"published-print":{"date-parts":[[2002,7]]},"abstract":"<jats:p>\n                    The small genome Mollicutes whose DNAs are completely sequenced (\n                    <jats:italic toggle=\"yes\">Mycoplasma genitalium<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">Mycoplasma pneumoniae<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">Mycoplasma pulmonis<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">Ureaplasma urealyticum<\/jats:italic>\n                    [\n                    <jats:italic toggle=\"yes\">parvum<\/jats:italic>\n                    ])\n         lack a gene (\n                    <jats:italic toggle=\"yes\">ndk<\/jats:italic>\n                    ) for the presumably essential nucleoside diphosphate kinase (NDPK). We hypothesized that other activities might replace NDPK activity. We found in\n                    <jats:italic toggle=\"yes\">M. genitalium<\/jats:italic>\n                    G37\n                    <jats:sup>T<\/jats:sup>\n                    ,\n                    <jats:italic toggle=\"yes\">Mycoplasma pneumoniae<\/jats:italic>\n                    FH\n                    <jats:sup>T<\/jats:sup>\n                    ,\n                    <jats:italic toggle=\"yes\">Mycoplasma fermentans<\/jats:italic>\n                    PG18\n                    <jats:sup>T<\/jats:sup>\n                    , and\n                    <jats:italic toggle=\"yes\">Mycoplasma capricolum<\/jats:italic>\n                    subsp.\n                    <jats:italic toggle=\"yes\">capricolum<\/jats:italic>\n                    Kid\n                    <jats:sup>T<\/jats:sup>\n                    that their 6-phosphofructokinases (6-PFKs),\n         phosphoglycerate kinases (PGKs), pyruvate kinases (PKs), and acetate kinases (AKs), besides reactant ADP\/ATP, could use other ribo- and deoxyribo-purine and pyrimidine NDPs and NTPs. These activities could\n         compensate for the absence of an orthologous\n                    <jats:italic toggle=\"yes\">ndk<\/jats:italic>\n                    gene in the Mycoplasmataceae. They suggest a metabolically varied and consequential role for unrelated and perhaps unsuspected \"replacement\" or compensatory\n         enzymes that may confound metabolic prediction. We partially purified and biochemically characterized the PKs, 6-PFKs, PGKs, and AKs from\n                    <jats:italic toggle=\"yes\">M. capricolum<\/jats:italic>\n                    subsp.\n                    <jats:italic toggle=\"yes\">capricolum<\/jats:italic>\n                    Kid\n                    <jats:sup>T<\/jats:sup>\n                    and\n                    <jats:italic toggle=\"yes\">M<\/jats:italic>\n                    .\n                    <jats:italic toggle=\"yes\">fermentans<\/jats:italic>\n                    PG18\n                    <jats:sup>T<\/jats:sup>\n                    .\n                  <\/jats:p>","DOI":"10.1089\/15362310260256909","type":"journal-article","created":{"date-parts":[[2002,11,11]],"date-time":"2002-11-11T15:45:12Z","timestamp":1037029512000},"page":"247-258","source":"Crossref","is-referenced-by-count":52,"title":["Suspected Utility of Enzymes with Multiple Activities in the Small Genome\n                    <i>Mycoplasma<\/i>\n                    Species: The Replacement of the Missing \"Household\" Nucleoside Diphosphate Kinase Gene and Activity by Glycolytic          Kinases"],"prefix":"10.1177","volume":"6","author":[{"given":"J. Dennis","family":"Pollack","sequence":"first","affiliation":[{"name":"Department of Molecular Virology, Immunology, and Medical Genetics, The College of Medicine and Public Health, The Ohio State University, Columbus, Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa A.","family":"Myers","sequence":"additional","affiliation":[{"name":"Department of Molecular Virology, Immunology, and Medical Genetics, The College of Medicine and Public Health, The Ohio State University, Columbus, Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Dandekar","sequence":"additional","affiliation":[{"name":"EMBL, Heidelberg, Germany; Institute of Molecular Medicine, University of Freiburg, Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Herrmann","sequence":"additional","affiliation":[{"name":"Zentrum f\u00fcr Molekulare Biologie Heidelberg, Mikrobiologie, Universit\u00e4t Heidelberg, Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2002,7]]},"container-title":["OMICS: A Journal of Integrative Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/15362310260256909","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/15362310260256909","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T17:39:38Z","timestamp":1771263578000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/15362310260256909"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,7]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2002,7]]}},"alternative-id":["10.1089\/15362310260256909"],"URL":"https:\/\/doi.org\/10.1089\/15362310260256909","relation":{},"ISSN":["1536-2310","1557-8100"],"issn-type":[{"value":"1536-2310","type":"print"},{"value":"1557-8100","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,7]]}}}