{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T05:20:05Z","timestamp":1777526405596,"version":"3.51.4"},"reference-count":0,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2008,4,1]],"date-time":"2008-04-01T00:00:00Z","timestamp":1207008000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ASSAY and Drug Development Technologies"],"published-print":{"date-parts":[[2008,4]]},"abstract":"\n Abstract:<\/jats:bold>\n Secondary active glucose transport is mediated by at least four members of the solute-linked carrier 5 gene family (sodium\/glucose transporter [SGLT] 1\u20134). Human genetic disorders of SGLTs including glucose-galactose malabsorption and familial renal glucosuria have increased attention on members of this family of transporters as putative drug targets. Using human SGLT1 (hSGLT1) as a paradigm, we developed a functional assay that should be adaptable to ultra-high-throughput operation and to other SGLTs. Human embryonic kidney (HEK) 293 cells stably expressing hSGLT1 (hSGLT1\/HEK293 cells) display a Na\n +<\/jats:sup>\n -dependent, phlorizin-sensitive\n \u03b1<\/jats:italic>\n -methyl-\n D<\/jats:sc>\n -[\n 14<\/jats:sup>\n C]glucopyranoside flux with expected kinetic parameters. In electrophysiological studies with hSGLT1\/HEK293 cells, substrate-dependent changes in membrane potential were observed, consistent with the electrogenic operation of hSGLT1. With the use of voltage-sensitive dyes, a membrane potential, fluorescence resonance energy transfer-based functional assay on a voltage\/ion probe reader platform has been established for SGLT1. This high-capacity functional assay displays similar characteristics in terms of substrate specificity and phlorizin sensitivity to those determined by more traditional approaches and should provide a means to identify novel and selective SGLT inhibitors.\n <\/jats:p>","DOI":"10.1089\/adt.2008.125","type":"journal-article","created":{"date-parts":[[2008,5,9]],"date-time":"2008-05-09T14:47:25Z","timestamp":1210344445000},"page":"255-262","source":"Crossref","is-referenced-by-count":4,"title":["A High-Capacity Membrane Potential FRET-Based Assay for the Sodium-Coupled Glucose Co-transporter SGLT1"],"prefix":"10.1177","volume":"6","author":[{"given":"Adam B.","family":"Weinglass","sequence":"first","affiliation":[{"name":"Department of Ion Channels, Merck Research Laboratories, Rahway, NJ."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew M.","family":"Swensen","sequence":"additional","affiliation":[{"name":"Department of Ion Channels, Merck Research Laboratories, Rahway, NJ."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Ion Channels, Merck Research Laboratories, Rahway, NJ."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William","family":"Schmalhofer","sequence":"additional","affiliation":[{"name":"Department of Ion Channels, Merck Research Laboratories, Rahway, NJ."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anu","family":"Thomas","sequence":"additional","affiliation":[{"name":"Department of Ion Channels, Merck Research Laboratories, Rahway, NJ."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brande","family":"Williams","sequence":"additional","affiliation":[{"name":"Department of Ion Channels, Merck Research Laboratories, Rahway, NJ."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leila","family":"Ross","sequence":"additional","affiliation":[{"name":"Department of Ion Channels, Merck Research Laboratories, Rahway, NJ."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristina","family":"Hashizume","sequence":"additional","affiliation":[{"name":"Department of Ion Channels, Merck Research Laboratories, Rahway, NJ."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Kohler","sequence":"additional","affiliation":[{"name":"Department of Ion Channels, Merck Research Laboratories, Rahway, NJ."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory J.","family":"Kaczorowski","sequence":"additional","affiliation":[{"name":"Department of Ion Channels, Merck Research Laboratories, Rahway, NJ."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria L.","family":"Garcia","sequence":"additional","affiliation":[{"name":"Department of Ion Channels, Merck Research Laboratories, Rahway, NJ."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2008,4,1]]},"container-title":["ASSAY and Drug Development Technologies"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/adt.2008.125","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/adt.2008.125","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T16:29:45Z","timestamp":1777393785000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/adt.2008.125"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,4]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2008,4]]}},"alternative-id":["10.1089\/adt.2008.125"],"URL":"https:\/\/doi.org\/10.1089\/adt.2008.125","relation":{},"ISSN":["1540-658X","1557-8127"],"issn-type":[{"value":"1540-658X","type":"print"},{"value":"1557-8127","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,4]]}}}