{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T01:12:18Z","timestamp":1790903538863,"version":"4.1.0"},"reference-count":0,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[1991,5,1]],"date-time":"1991-05-01T00:00:00Z","timestamp":673056000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["AIDS Research and Human Retroviruses"],"published-print":{"date-parts":[[1991,5]]},"abstract":"<jats:p>\n                    The vaccinia virus expression system was used to determine the role of human immunodeficiency virus type 1 (HIV-1) protease in viral morphogenesis and maturation. The unprocessed p55\n                    <jats:italic toggle=\"yes\">gag<\/jats:italic>\n                    precursor polyprotein alone was assembled to form HIV-1 particles which budded from cells. The particles were spherical and immature, containing an electron-dense shell in the particle submembrane; there was no evidence of core formation. Expression of both\n                    <jats:italic toggle=\"yes\">gag<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">pol<\/jats:italic>\n                    proteins from a recombinant containing the complete\n                    <jats:italic toggle=\"yes\">gag-pol<\/jats:italic>\n                    coding sequences resulted in intracellular processing of\n                    <jats:italic toggle=\"yes\">gag-pol<\/jats:italic>\n                    proteins and the production of mature particles with electrondense cores characteristic of wild-type HIV virions. To ascertain the role of protein processing in particle maturation, the\n                    <jats:italic toggle=\"yes\">pol<\/jats:italic>\n                    ORF in the\n                    <jats:italic toggle=\"yes\">gag-pol<\/jats:italic>\n                    recombinant was truncated to limit expression of the\n                    <jats:italic toggle=\"yes\">pol<\/jats:italic>\n                    gene to the protease domain. With this recombinant expressing p55\n                    <jats:italic toggle=\"yes\">gag<\/jats:italic>\n                    and protease, intracellular processing was observed. Some of the resultant particles were partially mature and contained processed\n                    <jats:italic toggle=\"yes\">gag<\/jats:italic>\n                    protein subunits. In contrast, particle maturation was not observed when the HIV-1 protease and p55\n                    <jats:italic toggle=\"yes\">gag<\/jats:italic>\n                    were coexpressed from separate recombinants, despite evidence of intracellular\n                    <jats:italic toggle=\"yes\">gag<\/jats:italic>\n                    processing. These findings suggest that HIV-1 protease must be an integral component of the full-length\n                    <jats:italic toggle=\"yes\">gag-pol<\/jats:italic>\n                    precursor for optimal processing and virion maturation.\n                  <\/jats:p>","DOI":"10.1089\/aid.1991.7.475","type":"journal-article","created":{"date-parts":[[2009,3,16]],"date-time":"2009-03-16T12:04:01Z","timestamp":1237205041000},"page":"475-483","source":"Crossref","is-referenced-by-count":23,"title":["Maturation of Human Immunodeficiency Virus Particles Assembled from the\n                    <i>gag<\/i>\n                    Precursor Protein Requires In Situ Processing by\n                    <i>gag-pol<\/i>\n                    Protease"],"prefix":"10.1177","volume":"7","author":[{"given":"Elizabeth K.","family":"Ross","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas R.","family":"Fuerst","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan M.","family":"Orenstein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"O'Neill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malcolm A.","family":"Martin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sundararajan","family":"Venkatesan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[1991,5,1]]},"container-title":["AIDS Research and Human Retroviruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/aid.1991.7.475","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/aid.1991.7.475","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T15:31:05Z","timestamp":1774625465000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/aid.1991.7.475"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,5]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1991,5]]}},"alternative-id":["10.1089\/aid.1991.7.475"],"URL":"https:\/\/doi.org\/10.1089\/aid.1991.7.475","relation":{},"ISSN":["0889-2229","1931-8405"],"issn-type":[{"value":"0889-2229","type":"print"},{"value":"1931-8405","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,5]]}}}