{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T16:18:04Z","timestamp":1774628284886,"version":"3.50.1"},"reference-count":0,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[1992,5,1]],"date-time":"1992-05-01T00:00:00Z","timestamp":704678400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["AIDS Research and Human Retroviruses"],"published-print":{"date-parts":[[1992,5]]},"abstract":"<jats:p>\n                    Multiple HIV-1\n                    <jats:italic toggle=\"yes\">nef<\/jats:italic>\n                    genes were cloned from lymphocyte DNA of asymptomatic seropositive individuals by polymerase chain reaction (PCR). Sequence analysis of these clones revealed a unique set of\n                    <jats:italic toggle=\"yes\">nef<\/jats:italic>\n                    variants with premature terminations (PCR\n                    <jats:italic toggle=\"yes\">nef<\/jats:italic>\n                    1 and 6), mutations at sites of potential posttranslational modification (PCR\n                    <jats:italic toggle=\"yes\">nef<\/jats:italic>\n                    2 and 3) and deletions. In common with laboratory isolates of\n                    <jats:italic toggle=\"yes\">nef<\/jats:italic>\n                    , strong sequence conservation was observed in the central domain of\n                    <jats:italic toggle=\"yes\">nef<\/jats:italic>\n                    and in the myristylation target sequence, with variable domains toward the N- and C-termini of the molecule. The biochemical function of\n                    <jats:italic toggle=\"yes\">nef<\/jats:italic>\n                    remains elusive however, as the products of these genes cloned into a bacterial expression system failed to reveal any nucleotide binding activity.\n                  <\/jats:p>","DOI":"10.1089\/aid.1992.8.537","type":"journal-article","created":{"date-parts":[[2009,3,16]],"date-time":"2009-03-16T12:42:49Z","timestamp":1237207369000},"page":"537-543","source":"Crossref","is-referenced-by-count":17,"title":["In Vivo Derived HIV-1\n                    <i>nef<\/i>\n                    Gene Products Are Heterogeneous and Lack Detectable Nucleotide Binding Activity"],"prefix":"10.1177","volume":"8","author":[{"given":"Mark","family":"Harris","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Hislop","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Panagiotis","family":"Patsilinacos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James C.","family":"Neil","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1992,5,1]]},"container-title":["AIDS Research and Human Retroviruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/aid.1992.8.537","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/aid.1992.8.537","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T15:42:27Z","timestamp":1774626147000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/aid.1992.8.537"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,5]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1992,5]]}},"alternative-id":["10.1089\/aid.1992.8.537"],"URL":"https:\/\/doi.org\/10.1089\/aid.1992.8.537","relation":{},"ISSN":["0889-2229","1931-8405"],"issn-type":[{"value":"0889-2229","type":"print"},{"value":"1931-8405","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,5]]}}}