{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T15:43:25Z","timestamp":1774626205735,"version":"3.50.1"},"reference-count":0,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[1994,2,1]],"date-time":"1994-02-01T00:00:00Z","timestamp":760060800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["AIDS Research and Human Retroviruses"],"published-print":{"date-parts":[[1994,2]]},"abstract":"<jats:p>\n                    In these studies, neonatal C.B-17 severe combined immunodeficient (nSCID) mice were reconstituted with human cord blood leukocytes (hu-CBLs). The resulting hu-CBL-nSCID mice contained readily detectable human CD3\n                    <jats:sup>+<\/jats:sup>\n                    T lymphocytes and CD20\n                    <jats:sup>+<\/jats:sup>\n                    human B cells, and produced substantial levels of human IgM and IgG (including all subclasses). Human cells persisted in lymphoid organs and peripheral blood for at least 8 weeks, and CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells outnumbered CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells. Engraftment of human cells in peripheral lymphoid organs and blood was much greater than that seen in adult SCID mice grafted with adult peripheral blood leukocytes (PBLs). Hu-CBL-nSCID mice were susceptible to infection with laboratory-adapted and fresh clinical human immunodeficiency virus type 1 (HIV-1) isolates. Following infection with HIV-1, virus could be recovered by the coculture of spleen, lymph node, peritoneal cavity, liver, and plasma samples from hu-CBL-nSCID mice with fresh human peripheral blood mononuclear cells, and proviral copies were detectable following amplification using the polymerase chain reaction (PCR). HIV p24 core antigen levels in hu-CBL-nSCID mouse plasma were consistent with ongoing viral replication and high viral burdens. Rapid CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cell depletion occurred following infection with laboratory isolates of HIV-1 or a syncytium-inducing clinical isolate, but a non-syncytium-inducing clinical isolate caused expansion of CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells, leading to an inversion of the CD4:CD8 ratio with only a transient decrease in CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells. These results suggest that the hu-CBL-nSCID mouse system has unique features that mimic certain aspects of pediatric HIV infection, and distinguish it from other animal models of HIV infection, including the related hu-PBL-SCID model.\n                  <\/jats:p>","DOI":"10.1089\/aid.1994.10.131","type":"journal-article","created":{"date-parts":[[2009,3,16]],"date-time":"2009-03-16T14:41:32Z","timestamp":1237214492000},"page":"131-141","source":"Crossref","is-referenced-by-count":12,"title":["Human Immunodeficiency Virus Type 1 Infection of Neonatal Severe Combined Immunodeficient Mice Xenografted with Human Cord Blood Cells"],"prefix":"10.1177","volume":"10","author":[{"given":"Barbara","family":"Reinhardt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce E.","family":"Torbett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard J.","family":"Gulizia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter P.","family":"Reinhardt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen A.","family":"Spector","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald E.","family":"Mosier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1994,2,1]]},"container-title":["AIDS Research and Human Retroviruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/aid.1994.10.131","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/aid.1994.10.131","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T14:21:29Z","timestamp":1774621289000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/aid.1994.10.131"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,2]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1994,2]]}},"alternative-id":["10.1089\/aid.1994.10.131"],"URL":"https:\/\/doi.org\/10.1089\/aid.1994.10.131","relation":{},"ISSN":["0889-2229","1931-8405"],"issn-type":[{"value":"0889-2229","type":"print"},{"value":"1931-8405","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,2]]}}}