{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T15:45:39Z","timestamp":1774626339923,"version":"3.50.1"},"reference-count":0,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[1994,11,1]],"date-time":"1994-11-01T00:00:00Z","timestamp":783648000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["AIDS Research and Human Retroviruses"],"published-print":{"date-parts":[[1994,11]]},"abstract":"<jats:p>\n                    Replication of the human immunodeficiency virus (HIV) is regulated tightly by the\n                    <jats:italic toggle=\"yes\">tat<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">rev<\/jats:italic>\n                    genes. The\n                    <jats:italic toggle=\"yes\">tat<\/jats:italic>\n                    gene of HIV is a potent\n                    <jats:italic toggle=\"yes\">trans<\/jats:italic>\n                    -activator of virus gene expression.\n                    <jats:italic toggle=\"yes\">trans<\/jats:italic>\n                    -Activation is mediated through the\n                    <jats:italic toggle=\"yes\">tat<\/jats:italic>\n                    -responsive element (TAR). Tat also has been shown to affect transcription of cellular genes and to\n                    <jats:italic toggle=\"yes\">trans<\/jats:italic>\n                    -activate other viral promoters. In transgenic animals,\n                    <jats:italic toggle=\"yes\">tat<\/jats:italic>\n                    expression in skin was implicated in the development of lesions resembling Kaposi's sarcoma (KS). More recently, evidence has been presented that suggests that Tat might play a role in the maintenance of KS cells. To study the possible role(s) of Tat in pathogenesis and disease progression, we have developed a retroviral vector for the transfer of\n                    <jats:italic toggle=\"yes\">tat<\/jats:italic>\n                    into murine bone marrow cells. We used this transduced bone marrow to repopulate recipient animals, which expressed the\n                    <jats:italic toggle=\"yes\">tat<\/jats:italic>\n                    gene in peripheral blood 6 months after transplantation as determined by PCR amplification of first-strand cDNA. Analysis of the hematopoietic tissues of mice 6 months posttransplantation indicated persistence of the\n                    <jats:italic toggle=\"yes\">tat<\/jats:italic>\n                    gene and its expression in thymus, lymph nodes, spleen, bone marrow, and peripheral blood. Although\n                    <jats:italic toggle=\"yes\">tat<\/jats:italic>\n                    expression was sustained in all hematopoietic tissues, no gross abnormalities were observed. The presence of\n                    <jats:italic toggle=\"yes\">tat<\/jats:italic>\n                    in all hematopoietic tissues strongly suggests transduction of stem or multipotential progenitor cells.\n                  <\/jats:p>","DOI":"10.1089\/aid.1994.10.1517","type":"journal-article","created":{"date-parts":[[2009,3,16]],"date-time":"2009-03-16T13:58:25Z","timestamp":1237211905000},"page":"1517-1524","source":"Crossref","is-referenced-by-count":5,"title":["Retrovirus-Mediated Transfer and Long-Term Expression of HIV Type 1\n                    <i>tat<\/i>\n                    Gene in Murine Hematopoietic Tissues"],"prefix":"10.1177","volume":"10","author":[{"given":"Amy L.B.","family":"Frazier","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. Victor","family":"Garcia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1994,11,1]]},"container-title":["AIDS Research and Human Retroviruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/aid.1994.10.1517","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/aid.1994.10.1517","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T14:24:33Z","timestamp":1774621473000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/aid.1994.10.1517"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,11]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[1994,11]]}},"alternative-id":["10.1089\/aid.1994.10.1517"],"URL":"https:\/\/doi.org\/10.1089\/aid.1994.10.1517","relation":{},"ISSN":["0889-2229","1931-8405"],"issn-type":[{"value":"0889-2229","type":"print"},{"value":"1931-8405","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,11]]}}}