{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T12:44:45Z","timestamp":1777380285639,"version":"3.51.4"},"reference-count":41,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2015,8,1]],"date-time":"2015-08-01T00:00:00Z","timestamp":1438387200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["AIDS Research and Human Retroviruses"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:p>\n                    Highly active antiretroviral therapy (HAART) contributed to the improvement in the life expectancy of HIV-infected patients. However, the emergence of drug-resistant mutations (DRM) is a major viral factor impacting therapeutic failure. Differences in DRM can occur among HIV-1 subtypes. We evaluate the kinetics of the selection of resistance mutations\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    analyzing two chimeric clones that contain the reverse transcriptases of subtypes B or C (RTB\u2032 and RTC\u2032) in cells treated with increasing concentrations of tenofovir disoproxil fumarate (TDF) and didanosine (ddI). The mutation K65R is selected more quickly in RTC\u2032 than in RTB\u2032 viruses with TDF and ddI, and additional mutations (positions 45, 62, and 68) were selected after K65R fixation. Other primary mutations (M184V and Q151M) were selected with ddI treatment in conjunction with K65R only in RTC\u2032 viruses. Both patterns, M184V+K65R and Q151M+K65R, have a significant impact on NRTI resistance. Our data suggest that selection of TDF and ddI DRMs can occur earlier in subtype C HIV in patients when compared to subtype B.\n                  <\/jats:p>","DOI":"10.1089\/aid.2014.0324","type":"journal-article","created":{"date-parts":[[2015,6,15]],"date-time":"2015-06-15T08:32:21Z","timestamp":1434357141000},"page":"851-858","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Short Communication:\n                    <i>In Vitro<\/i>\n                    Accumulation of Drug Resistance Mutations in Chimeric Infectious Clones Containing Subtype B or C Reverse Transcriptase and Selected with Tenofovir or Didanosine"],"prefix":"10.1177","volume":"31","author":[{"given":"Rodrigo D.","family":"Cunha","sequence":"first","affiliation":[{"name":"Universidade Federal do Rio de Janeiro","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Celina M.","family":"Abreu","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio de Janeiro","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arielly K.P.","family":"Sousa","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio de Janeiro","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viviana C.J.","family":"Mabombo","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio de Janeiro","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monique","family":"Nijhuis","sequence":"additional","affiliation":[{"name":"University Medical Center Utrecht","place":["the Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorien","family":"de Jong","sequence":"additional","affiliation":[{"name":"University Medical Center Utrecht","place":["the Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amilcar","family":"Tanuri","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio de Janeiro","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2015,8,1]]},"reference":[{"key":"e_1_3_3_2_2","unstructured":"(UNAIDS) UJP on H: Global Report: UNAIDS Report on the Global AIDS Epidemic 2013."},{"key":"e_1_3_3_3_2","unstructured":"WHO: Antiretroviral therapy for HIV infection in adults and adolescents. 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