{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T12:44:40Z","timestamp":1777380280771,"version":"3.51.4"},"reference-count":15,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2015,7,1]],"date-time":"2015-07-01T00:00:00Z","timestamp":1435708800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2015,7,1]],"date-time":"2015-07-01T00:00:00Z","timestamp":1435708800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["AIDS Research and Human Retroviruses"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:p>\n                    Structured treatment interruption (STI) has been trialed as an alternative to lifelong antiretroviral therapy (ART). We retrospectively performed single genome sequencing of the HIV-1\n                    <jats:italic toggle=\"yes\">pol<\/jats:italic>\n                    region from three patients representing different scenarios. They were either failing on continuous therapy (CT-F), failing STI (STI-F), or suppressing on STI (STI-S). Over 460 genomes were generated from three to five different time points over a 2-year period. We found multiple-linked-resistant mutations in both treatment failures. However, the CT-F patient showed a stepwise accumulation of diverse, linked mutations whereas the STI-F patient had lineage turnover between treatment periods with recirculation of wild-type and resistant variants from reservoirs. The STI-F patient showed a 7-fold increase in the third codon position substitution rate relative to the first and second positions compared to a 2-fold increase for CT-F and increased purifying selection in the\n                    <jats:italic toggle=\"yes\">pol<\/jats:italic>\n                    gene (62 vs. 22 sites, respectively). An understanding of intrapatient viral dynamics could guide the future direction of treatment interruption strategies.\n                  <\/jats:p>","DOI":"10.1089\/aid.2015.0035","type":"journal-article","created":{"date-parts":[[2015,5,8]],"date-time":"2015-05-08T08:01:04Z","timestamp":1431072064000},"page":"749-756","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Intrapatient Evolutionary Dynamics of Human Immunodeficiency Virus Type 1 in Individuals Undergoing Alternative Treatment Strategies with Reverse Transcriptase Inhibitors"],"prefix":"10.1177","volume":"31","author":[{"given":"Jonathan K.","family":"Kayondo","sequence":"first","affiliation":[{"name":"Uganda Virus Research Institute (UVRI)","place":["Uganda"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicaise","family":"Ndembi","sequence":"additional","affiliation":[{"name":"Medical Research Council 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