{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T06:21:36Z","timestamp":1790490096803,"version":"4.1.0"},"reference-count":11,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["AIDS Research and Human Retroviruses"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:p>\n                    Combined antiretroviral therapy (cART) does not eradicate HIV, which persists for years and can re-establish replication if treatment is stopped. The current challenge is identifying those tissues harboring virus through cART. Here, we used HIV\n                    <jats:italic toggle=\"yes\">env-nef<\/jats:italic>\n                    single genome sequencing and HIV\n                    <jats:italic toggle=\"yes\">gag<\/jats:italic>\n                    droplet digital PCR (ddPCR) to survey 50 tissues from five subjects on cART with no detectable plasma viral load at death. The spleen most consistently contained multiple proviral and expressed sequences (4\/5 participants). Spleen-derived HIV demonstrated two distinct phylogenetic patterns: multiple identical sequences, often from different tissues, as well as diverse viral sequences on long terminal branches. Our results suggested that ddPCR may overestimate the size of the tissue-based viral reservoir. The spleen, a lymphatic organ at the intersection of the immune and circulatory systems, may play a key role in viral persistence.\n                  <\/jats:p>","DOI":"10.1089\/aid.2017.0254","type":"journal-article","created":{"date-parts":[[2017,10,31]],"date-time":"2017-10-31T02:57:00Z","timestamp":1509418620000},"page":"123-125","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":51,"title":["The Spleen Is an HIV-1 Sanctuary During Combined Antiretroviral Therapy"],"prefix":"10.1177","volume":"34","author":[{"given":"David J.","family":"Nolan","sequence":"first","affiliation":[{"name":"BioInfoExperts LLC"},{"name":"University of Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca","family":"Rose","sequence":"additional","affiliation":[{"name":"BioInfoExperts LLC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia H.","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"University of Florida"},{"name":"University of Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Salemi","sequence":"additional","affiliation":[{"name":"University of Florida"},{"name":"University of Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elyse J.","family":"Singer","sequence":"additional","affiliation":[{"name":"University of California Los Angeles David Geffen School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanna L.","family":"Lamers","sequence":"additional","affiliation":[{"name":"BioInfoExperts LLC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael S.","family":"McGrath","sequence":"additional","affiliation":[{"name":"University of California San Francisco"},{"name":"Departments of Laboratory Medicine, Pathology, and Medicine, University of California San Francisco, San Francisco, California."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2018,1,1]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/COH.0000000000000281"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00684-16"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00674-16"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.0305-1846.2001.00334.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.2334"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI80567"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1318249111"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature16933"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.1772"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri1669"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1080\/01926230600867743"}],"container-title":["AIDS Research and Human Retroviruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/aid.2017.0254","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/aid.2017.0254","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/aid.2017.0254","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T09:13:19Z","timestamp":1777367599000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/aid.2017.0254"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1]]},"references-count":11,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,1]]}},"alternative-id":["10.1089\/aid.2017.0254"],"URL":"https:\/\/doi.org\/10.1089\/aid.2017.0254","relation":{},"ISSN":["0889-2229","1931-8405"],"issn-type":[{"value":"0889-2229","type":"print"},{"value":"1931-8405","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,1]]}}}