{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T12:37:30Z","timestamp":1777379850626,"version":"3.51.4"},"reference-count":22,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["AIDS Research and Human Retroviruses"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:p>\n                    The nucleoside reverse transcriptase inhibitor abacavir (\n                    <jats:sc>ABC<\/jats:sc>\n                    ) is used commonly to treat young children with HIV infection and is a component of the fixed-dose-combination Triumeq\n                    <jats:sup>\u00ae<\/jats:sup>\n                    .\n                    <jats:sc>ABC<\/jats:sc>\n                    can trigger a severe hypersensitivity reaction in people who are homozygous or heterozygous for\n                    <jats:italic toggle=\"yes\">HLA-B*57:01<\/jats:italic>\n                    . Testing for\n                    <jats:italic toggle=\"yes\">HLA-B*57:01<\/jats:italic>\n                    before\n                    <jats:sc>ABC<\/jats:sc>\n                    initiation is standard-of-care in high-resource settings, but current tests are costly or difficult to access in resource-limited settings. To address these gaps, we developed an inexpensive simple-to-use rapid assay to detect\n                    <jats:italic toggle=\"yes\">HLA-B*57:01<\/jats:italic>\n                    . We designed and optimized a multiplexed polymerase chain reaction (PCR) to amplify\n                    <jats:italic toggle=\"yes\">HLA-B*57<\/jats:italic>\n                    subtypes and the human beta-globin gene; employed probes and ligation to specifically tag the\n                    <jats:italic toggle=\"yes\">HLA-B*57:01<\/jats:italic>\n                    allele with biotin. Tagged-ligated products were detected by immunocapture in an enzyme-linked immunosorbent assay plate or lateral flow strip. Cell lines with known HLA genotypes were used to optimize the assay. The optimized assay was then compared with genotypes of clinical specimens (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    \u2009=\u200960) determined by sequencing, with specimens enriched for individuals with\n                    <jats:italic toggle=\"yes\">HLA-B*57:01<\/jats:italic>\n                    . The optimized assay utilizes 40-min 35-cycle multiplex PCR for\n                    <jats:italic toggle=\"yes\">B*57<\/jats:italic>\n                    and beta-globin; 20-min ligation reaction; and 15-min detection. Evaluation of the\n                    <jats:italic toggle=\"yes\">HLA-B*57:01<\/jats:italic>\n                    oligonucleotide ligation assay using clinical specimens had a sensitivity of 100% (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    \u2009=\u200927\/27 typed as\n                    <jats:italic toggle=\"yes\">B*57:01<\/jats:italic>\n                    ) and specificity of 100% (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    \u2009=\u200933\/33 typed as non-\n                    <jats:italic toggle=\"yes\">B*57:01<\/jats:italic>\n                    ) by visual interpretation of lateral flow strips. The cost is US$5.96\/specimen. This rapid and economical assay accurately detects\n                    <jats:italic toggle=\"yes\">HLA-B*57:01<\/jats:italic>\n                    in clinical specimens. Use of this assay could expand access to\n                    <jats:italic toggle=\"yes\">HLA-B*57:01<\/jats:italic>\n                    genotyping and facilitate safe same-day initiation of\n                    <jats:sc>ABC<\/jats:sc>\n                    -based treatment.\n                  <\/jats:p>","DOI":"10.1089\/aid.2021.0103","type":"journal-article","created":{"date-parts":[[2021,10,29]],"date-time":"2021-10-29T10:32:03Z","timestamp":1635503523000},"page":"930-935","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Rapid Near Point-of-Care Assay for\n                    <i>HLA-B*57:01<\/i>\n                    Genotype Associated with Severe Hypersensitivity Reaction to Abacavir"],"prefix":"10.1177","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8438-511X","authenticated-orcid":false,"given":"Jackson J.","family":"Wallner","sequence":"first","affiliation":[{"name":"Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingrid A.","family":"Beck","sequence":"additional","affiliation":[{"name":"Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nuttada","family":"Panpradist","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, University of Washington, Seattle, Washington, USA."},{"name":"Global Health of Women, Adolescents, and Children (Global WACh), School of Public Health, University of Washington, Seattle, Washington, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Parker S.","family":"Ruth","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, University of Washington, Seattle, Washington, USA."},{"name":"Paul G. Allen School of Computer Science and Engineering, University of Washington, Seattle, Washington, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Humberto","family":"Valenzuela-Ponce","sequence":"additional","affiliation":[{"name":"CIENI Centre for Research in Infectious Diseases, National Institute of Respiratory Diseases (INER), Mexico City, Mexico."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maribel","family":"Soto-Nava","sequence":"additional","affiliation":[{"name":"CIENI Centre for Research in Infectious Diseases, National Institute of Respiratory Diseases (INER), Mexico City, Mexico."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Santiago","family":"\u00c1vila-R\u00edos","sequence":"additional","affiliation":[{"name":"CIENI Centre for Research in Infectious Diseases, National Institute of Respiratory Diseases (INER), Mexico City, Mexico."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barry R.","family":"Lutz","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, University of Washington, Seattle, Washington, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9566-8959","authenticated-orcid":false,"given":"Lisa M.","family":"Frenkel","sequence":"additional","affiliation":[{"name":"Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA."},{"name":"Laboratory Medicine and Pathology, Global Health, and Medicine, Departments of Pediatrics, University of Washington, Seattle, Washington, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2021,12,15]]},"reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00002030-199816000-00001"},{"key":"e_1_3_5_3_2","unstructured":"Update of Recommendations on First- and Second-Line Antiretroviral Regimens. World Health Organization Geneva Switzerland 2019."},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.qai.0000147521.34369.c9"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0149-2918(01)80132-6"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)07873-X"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/clpt.2011.355"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.2005.00401.x"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.2174\/138920212800793311"},{"key":"e_1_3_5_10_2","first-page":"93","article-title":"Luminex technology for HLA typing by PCR-SSO and identification of HLA antibody specificities","volume":"2004","author":"Cesbron-Gautier A","year":"2004","unstructured":"Cesbron-Gautier A, , Simon P, , Achard L, , Cury S, , Follea G, , Bignon JD: Luminex technology for HLA typing by PCR-SSO and identification of HLA antibody specificities. Ann Biol Clin, 2004; 2004:93\u201398.","journal-title":"Ann Biol Clin"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01128-17"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-125-4-199608150-00001"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1097\/QAI.0b013e31817ed7d7"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0145962"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2019.11.002"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0000000000002524"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/iji.12122"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/tan.13811"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2013.06.025"},{"key":"e_1_3_5_20_2","first-page":"D783","article-title":"Allele frequency net database (AFND) 2020 update: Gold-standard data classification, open access genotype data and new query tools","volume":"48","author":"Gonzalez-Galarza FF","year":"2020","unstructured":"Gonzalez-Galarza FF, , McCabe A, , Santos EJ, et al.: Allele frequency net database (AFND) 2020 update: Gold-standard data classification, open access genotype data and new query tools. Nucleic Acids Res, 2020; 48:D783\u2013D788.","journal-title":"Nucleic Acids Res"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10156-013-0630-9"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2021.103236"},{"key":"e_1_3_5_23_2","unstructured":"Panel on Antiretroviral Therapy and Medical Management of Children Living with HIV : Guidelines for the use of antiretroviral agents in pediatric HIV infection. Available at http:\/\/aidsinfo.nih.gov\/content-files\/lvguidelines\/pediatricguidelines.pdf accessed March10 2021."}],"container-title":["AIDS Research and Human Retroviruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/aid.2021.0103","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/aid.2021.0103","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/aid.2021.0103","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T09:15:46Z","timestamp":1777367746000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/aid.2021.0103"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12]]},"references-count":22,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["10.1089\/aid.2021.0103"],"URL":"https:\/\/doi.org\/10.1089\/aid.2021.0103","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2021.05.26.21257187","asserted-by":"object"}]},"ISSN":["0889-2229","1931-8405"],"issn-type":[{"value":"0889-2229","type":"print"},{"value":"1931-8405","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12]]}}}