{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T02:26:35Z","timestamp":1783736795220,"version":"3.55.0"},"reference-count":16,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2021,9,8]],"date-time":"2021-09-08T00:00:00Z","timestamp":1631059200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2021,9,8]],"date-time":"2021-09-08T00:00:00Z","timestamp":1631059200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Appl Biosaf."],"published-print":{"date-parts":[[2022,3,1]]},"abstract":"\n Introduction:<\/jats:title>\n The applications of fumigation and the challenges that high-containment facilities face in achieving effective large volume decontamination are well understood. The Biosecurity Research Institute at Kansas State University sought to evaluate a novel system within their biosafety level 3 (BSL-3) and animal biosafety level 3 agriculture (ABSL-3Ag) facility.<\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n \n The system chosen for this study is the CURIS\n \u00ae<\/jats:sup>\n Hybrid Hydrogen Peroxide\n TM<\/jats:sup>\n (HHP\n TM<\/jats:sup>\n ) system, comprising a mobile 36-pound (16\u2009kg) device delivering a proprietary 7% hydrogen peroxide (H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n ) solution. To examine the system's efficacy in multiple laboratory settings, two BSL-3 laboratories (2,281 [65\u2009m\n 3<\/jats:sup>\n ] and 4,668 ft\n 3<\/jats:sup>\n [132\u2009m\n 3<\/jats:sup>\n ]) with dropped ceiling interstitial spaces and an ABSL-3Ag necropsy suite (44,212 ft\n 3<\/jats:sup>\n [1,252\u2009m\n 3<\/jats:sup>\n ]) with 21-foot (6.4\u2009m) ceilings were selected. Biological indicators (BIs) of\n Geobacillus stearothermophilus<\/jats:italic>\n (1.7\u2009\u00d7\u200910\n 6<\/jats:sup>\n organisms) on steel spore carriers and H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n chemical indicators (CIs) were used to provide validation.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n After cycle optimization, the smaller laboratory had a total of 60 BIs over two treatments that demonstrated a greater than 6-log reduction of bacterial spores. The larger laboratory (192 BIs) and the necropsy suite (206 BIs) had no BIs positive for spore growth when incubated at 60\u00b0C for 24\u2009h per manufacturer's specifications.<\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n \n Overall successful results through multiple components of this study demonstrate that the HHP device, paired with the pulsed 7% H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n solution, achieved efficacy regardless of variables in laboratory size and layout. Perceived challenges such as 21-ft (6.4\u2009m) ceiling heights, active equipment, and difficult to access ceiling interstitial spaces proved unfounded. Given the successful sterilization of all challenged BIs, the HHP system presents a useful alternative for high level decontamination within BSL-3 and ABSL-3Ag facilities.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1089\/apb.2021.0012","type":"journal-article","created":{"date-parts":[[2021,9,14]],"date-time":"2021-09-14T12:23:57Z","timestamp":1631622237000},"page":"7-14","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Analysis of Range and Use of a Hybrid Hydrogen Peroxide System for Biosafety Level 3 and Animal Biosafety Level 3 Agriculture Laboratory Decontamination"],"prefix":"10.1177","volume":"27","author":[{"given":"John R.","family":"Henneman","sequence":"first","affiliation":[{"name":"Biosecurity Research Institute, Kansas State University, Manhattan, Kansas, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth A.","family":"McQuade","sequence":"additional","affiliation":[{"name":"Biosecurity Research Institute, Kansas State University, Manhattan, Kansas, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachael R.","family":"Sullivan","sequence":"additional","affiliation":[{"name":"Biosecurity Research Institute, Kansas State University, Manhattan, Kansas, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jen","family":"Downard","sequence":"additional","affiliation":[{"name":"Biosecurity Research Institute, Kansas State University, Manhattan, Kansas, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashley","family":"Thackrah","sequence":"additional","affiliation":[{"name":"Biosecurity Research Institute, Kansas State University, Manhattan, Kansas, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1299-2153","authenticated-orcid":false,"given":"Meaghan","family":"Hislop","sequence":"additional","affiliation":[{"name":"Department of Science and Research, CURIS System, Oviedo, Florida, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,9,8]]},"reference":[{"key":"e_1_3_5_2_2","unstructured":"Meechan P Potts J. Biosafety in Microbiological and Biomedical Laboratories 6th Edition. U.S. Centers for Disease Control and Prevention (CDC); 2020. HHS Publication No. (CDC) 300859. https:\/\/www.cdc.gov\/labs\/pdf\/SF__19_308133-A_BMBL6_00-BOOK-WEB-final-3.pdf. Accessed March9 2021."},{"key":"e_1_3_5_3_2","doi-asserted-by":"crossref","unstructured":"Kaspari O Lemmer K Becker S et al. Decontamination of a BSL-3 laboratory by hydrogen peroxide fumigation using three different surrogates for Bacillus anthracis spores. J Appl Microbiol. 2014;117(4):1095\u20131103.","DOI":"10.1111\/jam.12601"},{"key":"e_1_3_5_4_2","unstructured":"Office of Pesticide Programs. Protocol for Room Sterilization by Fogger Application. U.S. Environmental Protection Agency (EPA); 2019. https:\/\/www.epa.gov\/sites\/production\/files\/2015-09\/documents\/room-sterilization.pdf. Accessed March9 2021."},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/153567601101600303"},{"key":"e_1_3_5_6_2","volume-title":"Disinfection, Sterilization and Preservation, 5th ed","author":"Joslyn LJ","year":"2001","unstructured":"Joslyn LJ, . Gaseous chemical sterilization. In: Block SS, ed. Disinfection, Sterilization and Preservation, 5th ed. Philadelphia, PA: Lippincott, Williams, & Wilkins; 2001."},{"key":"e_1_3_5_7_2","unstructured":"IS Med Specialties. Hydrogen Peroxide Compatibility Chart. IndustrialSpec. 2020. https:\/\/www.industrialspec.com\/images\/files\/hydrogen-peroxide-material-compatibility-chart-from-Ism.pdf. Accessed March11 2021."},{"key":"e_1_3_5_8_2","unstructured":"McAnoy AM Sait M Pantelidis S. Establishment of a Vaporous Hydrogen Peroxide Bio-Decontamination Capability. Victoria: Australian Government Department of Defense Defense Science and Technology Organization (AUS); 2007 16p. Report No. DSTO-TR-1994."},{"key":"e_1_3_5_9_2","unstructured":"Ghidoni D Grinstead F Held K Mullen R Thibeault R . Effectiveness of aerosolized hydrogen peroxide in simultaneous decontamination of a laboratory and a biological safety cabinet. CURIS Company Publication. 2018. https:\/\/www.curissystem.com. Accessed March10 2021."},{"key":"e_1_3_5_10_2","article-title":"Aerosolized hydrogen peroxide decontamination of N95 respirators, with fit-testing and virologic confirmation of suitability for re-use during the COVID-19 pandemic","author":"Derr TH","unstructured":"Derr TH, , James MA, , Kuny CV, et al. Aerosolized hydrogen peroxide decontamination of N95 respirators, with fit-testing and virologic confirmation of suitability for re-use during the COVID-19 pandemic. medRxiv. 10.1101\/2020.04.17.20068577J. Accessed March30, 2021.","journal-title":"medRxiv"},{"key":"e_1_3_5_11_2","unstructured":"Cirillo B Containing an outbreak at the ground level: 100% decrease of non-enveloped virus norovirus in a 1500 bed assisted living facility. CURIS Company Publication. 2020. https:\/\/www.curissystem.com. Accessed March10 2021."},{"key":"e_1_3_5_12_2","unstructured":"The National Institute for Occupational Safety and Health (NIOSH) Immediately Dangerous to Life or Health Concentrations (IDLH): Formaldehyde. U.S. Centers for Disease Control and Prevention (CDC); 2014. https:\/\/www.cdc.gov\/niosh\/idlh\/50000.html. Accessed March9 2021."},{"key":"e_1_3_5_13_2","unstructured":"The National Institute for Occupational Safety and Health (NIOSH) Immediately Dangerous to Life or Health Concentrations (IDLH): Hydrogen Peroxide. U.S. Centers for Disease Control and Prevention (CDC); 2014. https:\/\/www.cdc.gov\/niosh\/idlh\/772841.html. Accessed March9 2021."},{"key":"e_1_3_5_14_2","unstructured":"Office of Chemical Safety and Pollution Prevention CU RoxideTM Master Label. United States Environmental Protection Agency (EPA). 2020. https:\/\/www3.epa.gov\/pesticides\/chem_search\/ppls\/093324-00001-20200716.pdf. Accessed March10 2021."},{"key":"e_1_3_5_15_2","unstructured":"Microchem Laboratories. GLP Custom Viricidal Whole Room Fogger Sterilization: Feline calicivirus (EPA approved human norovirus surrogate). 2020. https:\/\/www.curissystem.com. Accessed March10 2021."},{"key":"e_1_3_5_16_2","unstructured":"Microchem Laboratories . GLP Evaluation of Surface Room Disinfection via Fogging Device Against C.difficile. 2019. https:\/\/www.curissystem.com. Accessed March10 2021."},{"key":"e_1_3_5_17_2","unstructured":"Aliucci J . Effective penetration of dry biofilm through pulse hydrogen peroxide. CURIS Company Publication. 2019. https:\/\/www.curissystem.com. 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