{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T18:54:14Z","timestamp":1792004054370,"version":"4.4.0"},"reference-count":29,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2021,9,8]],"date-time":"2021-09-08T00:00:00Z","timestamp":1631059200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Appl Biosaf."],"published-print":{"date-parts":[[2022,3,1]]},"abstract":"<jats:sec>\n                    <jats:title>Aim:<\/jats:title>\n                    <jats:p>This study aimed to validate the efficacy of hydrogen peroxide vapor (HPV) decontamination technology set up in a biosafety level 3 (BSL-3) laboratory on surrogates and hazard group 3 (HG3) agents.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and Results:<\/jats:title>\n                    <jats:p>\n                      The HPV decontamination system (Bioquell) was assessed with both qualitative and quantitative methods on (1) spore surrogates (\n                      <jats:italic toggle=\"yes\">Geobacillus stearothermophilus<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">Bacillus atrophaeus<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">Bacillus thuringiensis<\/jats:italic>\n                      ) in the BSL-3 laboratory and in the material airlock and on (2) HG3 agents (\n                      <jats:italic toggle=\"yes\">Bacillus anthracis;<\/jats:italic>\n                      SARS-CoV-2, Venezuelan equine encephalitis virus [VEE], and Vaccinia virus) in the BSL-3 laboratory. Other HG3 bacteria likely to be handled in the BSL-3 laboratory (\n                      <jats:italic toggle=\"yes\">Yersinia pestis<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">Burkholderia mallei<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">Brucella melitensis<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">Francisella tularensis<\/jats:italic>\n                      ) were excluded from the HPV decontamination assays as preliminary viability tests demonstrated the total inactivation of these agents after 48\u2009h drying on different materials.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>\n                      The efficacy of HPV decontamination was validated with a reduction in viability of 5\u20137 log\n                      <jats:sub>10<\/jats:sub>\n                      for the spores (surrogates and\n                      <jats:italic toggle=\"yes\">B. anthracis<\/jats:italic>\n                      ), and for the enveloped RNA viruses. Vaccinia showed a higher resistance to the decontamination process, being dependent on the biological indicator location in the BSL-3 laboratory.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/apb.2021.0022","type":"journal-article","created":{"date-parts":[[2021,9,8]],"date-time":"2021-09-08T14:44:15Z","timestamp":1631112255000},"page":"15-22","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Hydrogen Peroxide Vapor Decontamination of Hazard Group 3 Bacteria and Viruses in a Biosafety Level 3 Laboratory"],"prefix":"10.1177","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1432-3030","authenticated-orcid":false,"given":"Charlotte","family":"Falaise","sequence":"first","affiliation":[{"name":"DGA CBRN Defence Center, Biology Division, French Ministry of the Armed Forces, Vert-le-Petit, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"Bouvattier","sequence":"additional","affiliation":[{"name":"DGA CBRN Defence Center, Biology Division, French Ministry of the Armed Forces, Vert-le-Petit, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guilhem","family":"Larigauderie","sequence":"additional","affiliation":[{"name":"DGA CBRN Defence Center, Biology Division, French Ministry of the Armed Forces, Vert-le-Petit, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rie","family":"Lafontaine","sequence":"additional","affiliation":[{"name":"DGA CBRN Defence Center, Biology Division, French Ministry of the Armed Forces, Vert-le-Petit, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Berchebru","sequence":"additional","affiliation":[{"name":"DGA CBRN Defence Center, Biology Division, French Ministry of the Armed Forces, Vert-le-Petit, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audrey","family":"Marangon","sequence":"additional","affiliation":[{"name":"DGA CBRN Defence Center, Biology Division, French Ministry of the Armed Forces, Vert-le-Petit, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rie","family":"Vaude-Lauthier","sequence":"additional","affiliation":[{"name":"DGA CBRN Defence Center, Biology Division, French Ministry of the Armed Forces, Vert-le-Petit, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7oise","family":"Raynaud","sequence":"additional","affiliation":[{"name":"DGA CBRN Defence Center, Biology Division, French Ministry of the Armed Forces, Vert-le-Petit, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Taysse","sequence":"additional","affiliation":[{"name":"DGA CBRN Defence Center, Biology Division, French Ministry of the Armed Forces, Vert-le-Petit, France."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,9,8]]},"reference":[{"issue":"6","key":"e_1_3_5_2_2","first-page":"291","article-title":"Theoretical analysis of the condensation of hydrogen peroxide gas and water vapour as used in surface decontamination","volume":"56","author":"Watling D","year":"2002","unstructured":"Watling D, , Ryle C, , Parks M, , Christopher M. 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