{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T18:29:52Z","timestamp":1791052192452,"version":"4.1.0"},"reference-count":50,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2010,5,1]],"date-time":"2010-05-01T00:00:00Z","timestamp":1272672000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants &amp; Redox Signaling"],"published-print":{"date-parts":[[2010,5]]},"abstract":"<jats:p>\n                    The mechanisms responsible for the cardioprotective effect of hydrogen sulfide (H\n                    <jats:sub>2<\/jats:sub>\n                    S) are unclear. The present study was designed to examine whether H\n                    <jats:sub>2<\/jats:sub>\n                    S could regulate hyperhomocysteinemia (HHcy)-induced cardiomyocytic endoplasmic reticulum (ER) stress. A rat model of HHcy was produced, and H9c2 cells (rat embryonic heart\u2013derived cell line) were cultured. The plasma homocysteine was measured by using HPLC. Plasma H\n                    <jats:sub>2<\/jats:sub>\n                    S concentration and myocardial H\n                    <jats:sub>2<\/jats:sub>\n                    S production were measured with a sulfide-sensitive electrode. Confocal immunofluorescent analysis for cardiomyocytic C\/EBP homologous protein (CHOP) was performed. Glucose-regulated protein 78 (GRP78), CHOP, and caspase 12 expressions by myocardial tissues and cleaved caspase 12 and p-eIF2\u03b1 expressions by H9c2 cells were detected with Western blotting. The results showed that methionine overload induced HHcy, resulting in a marked cardiomyocytic ER stress, whereas endogenous production of H\n                    <jats:sub>2<\/jats:sub>\n                    S was reduced in rats with HHcy. H\n                    <jats:sub>2<\/jats:sub>\n                    S supplementation, however, decreased expressions of ER stress\u2013associated proteins, including GRP78, CHOP, and caspase 12, by myocardial tissues\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    . The inhibition of endogenous H\n                    <jats:sub>2<\/jats:sub>\n                    S production further enhanced cardiomyocytic ER stress, but H\n                    <jats:sub>2<\/jats:sub>\n                    S supplementation effectively antagonized the H9c2 cell CHOP, cleaved caspase 12 and p-eIF2\u03b1 expressions induced by Hcy, thapsigargin, or tunicamycin\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    . The results suggest that H\n                    <jats:sub>2<\/jats:sub>\n                    S can attenuate cardiomyocytic ER stress in HHcy-induced cardiomyocytic injury.\n                    <jats:italic toggle=\"yes\">Antioxid. Redox Signal.<\/jats:italic>\n                    12, 0000\u20130000.\n                  <\/jats:p>","DOI":"10.1089\/ars.2009.2898","type":"journal-article","created":{"date-parts":[[2009,9,21]],"date-time":"2009-09-21T10:08:12Z","timestamp":1253527692000},"page":"1079-1091","source":"Crossref","is-referenced-by-count":90,"title":["Hydrogen Sulfide Attenuates Hyperhomocysteinemia-Induced Cardiomyocytic Endoplasmic Reticulum Stress in Rats"],"prefix":"10.1177","volume":"12","author":[{"given":"Hongling","family":"Wei","sequence":"first","affiliation":[{"name":"Peking University First Hospital","place":["P.R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongyuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Peking University First Hospital","place":["P.R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongfang","family":"Jin","sequence":"additional","affiliation":[{"name":"Peking University First Hospital","place":["P.R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Die","family":"Liu","sequence":"additional","affiliation":[{"name":"Peking University First Hospital","place":["P.R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuying","family":"Tang","sequence":"additional","affiliation":[{"name":"Peking University First Hospital","place":["P.R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaoshu","family":"Tang","sequence":"additional","affiliation":[{"name":"Peking University First Hospital","place":["P.R. China"]},{"name":"Key Laboratory of Molecular Cardiovascular Diseases, Ministry of Education, Beijing, P.R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junbao","family":"Du","sequence":"additional","affiliation":[{"name":"Peking University First Hospital","place":["P.R. China"]},{"name":"Key Laboratory of Molecular Cardiovascular Diseases, Ministry of Education, Beijing, P.R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2010,5,1]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-007-0011-8"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/1358836X0501000105"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/aje\/154.2.150"},{"key":"e_1_3_3_5_2","first-page":"2199","article-title":"Evaluation and intermethod comparison of the Bio-Rad high-performance liquid chromatographic method for plasma total homocysteine","volume":"44","author":"Dias VC","year":"1998","unstructured":"Dias VC, , Bamforth FJ, , Tesanovic M, , Hyndman ME, , Parsons HG, , Cembrowski GS. Evaluation and intermethod comparison of the Bio-Rad high-performance liquid chromatographic method for plasma total homocysteine. Clin Chem, 44:2199\u20132201. 1998.","journal-title":"Clin Chem"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2007.1780"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22037"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00380-003-0743-7"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0705891104"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-09-03386.2002"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(86)90228-9"},{"key":"e_1_3_3_12_2","first-page":"545","article-title":"Application of sensitive sulphur electrode assay to measure and analyze cystathionine-gamma-lyase\/hydrogen sulfide pathway of cardiovascular tissues, cells and plasma in rats","volume":"37","author":"Geng B","year":"2005","unstructured":"Geng B, , Du JB, , Tang CS. Application of sensitive sulphur electrode assay to measure and analyze cystathionine-gamma-lyase\/hydrogen sulfide pathway of cardiovascular tissues, cells and plasma in rats. Beijing Da Xue Xue Bao, 37:545\u2013548. 2005.","journal-title":"Beijing Da Xue Xue Bao"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M501095200"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI10588"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.6878"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01552-06"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.05.147"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2007.11.003"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pathmechdis.3.121806.151434"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1681.2007.04812.x"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.99.1.178"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M505784200"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.4.1249-1259.2001"},{"key":"e_1_3_3_24_2","first-page":"111","article-title":"Vascular pathology of homocysteinemia: implications for the pathogenesis of arteriosclerosis","volume":"56","author":"McCully KS","year":"1969","unstructured":"McCully KS. Vascular pathology of homocysteinemia: implications for the pathogenesis of arteriosclerosis. 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