{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T03:10:57Z","timestamp":1791169857265,"version":"4.1.0"},"reference-count":71,"publisher":"SAGE Publications","issue":"16","license":[{"start":{"date-parts":[[2013,6,1]],"date-time":"2013-06-01T00:00:00Z","timestamp":1370044800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Antioxidants &amp; Redox Signaling"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:p>\n                    <jats:bold>\n                      <jats:italic toggle=\"yes\">Aims:<\/jats:italic>\n                    <\/jats:bold>\n                    Through its interaction with H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    , glutathione is a candidate for transmission of signals in plant responses to pathogens, but identification of signaling roles is complicated by its antioxidant function. Using a genetic approach based on a conditional catalase-deficient Arabidopsis mutant,\n                    <jats:italic toggle=\"yes\">cat2<\/jats:italic>\n                    , this study aimed at establishing whether GSH plays an important functional role in the transmission of signals downstream of H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    .\n                    <jats:bold>\n                      <jats:italic toggle=\"yes\">Results:<\/jats:italic>\n                    <\/jats:bold>\n                    Introducing the\n                    <jats:italic toggle=\"yes\">cad2<\/jats:italic>\n                    or allelic mutations in the glutathione synthesis pathway into\n                    <jats:italic toggle=\"yes\">cat2<\/jats:italic>\n                    blocked H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    -triggered GSH oxidation and accumulation. While no effects on NADP(H) or ascorbate were observed, and H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    -induced decreases in growth were maintained, blocking GSH modulation antagonized salicylic acid (SA) accumulation and SA-dependent responses. Other novel double and triple mutants were produced and compared with\n                    <jats:italic toggle=\"yes\">cat2 cad2<\/jats:italic>\n                    at the levels of phenotype, expression of marker genes, nontargeted metabolite profiling, accumulation of SA, and bacterial resistance. Most of the effects of the\n                    <jats:italic toggle=\"yes\">cad2<\/jats:italic>\n                    mutation on H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    -triggered responses were distinct from those produced by mutations for GLUTATHIONE REDUCTASE1 (\n                    <jats:italic toggle=\"yes\">GR1<\/jats:italic>\n                    ) or NONEXPRESSOR OF PATHOGENESIS-RELATED GENES 1 (\n                    <jats:italic toggle=\"yes\">NPR1<\/jats:italic>\n                    ), and were linked to compromised induction of ISOCHORISMATE SYNTHASE1 (\n                    <jats:italic toggle=\"yes\">ICS1<\/jats:italic>\n                    ) and ICS1-dependent SA accumulation.\n                    <jats:bold>\n                      <jats:italic toggle=\"yes\">Innovation:<\/jats:italic>\n                    <\/jats:bold>\n                    A novel genetic approach was used in which GSH content or antioxidative capacity was independently modified in an H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    signaling background. Analysis of new double and triple mutants allowed us to infer previously undescribed regulatory roles for GSH.\n                    <jats:bold>\n                      <jats:italic toggle=\"yes\">Conclusion:<\/jats:italic>\n                    <\/jats:bold>\n                    In parallel to its antioxidant role, GSH acts independently of NPR1 to allow increased intracellular H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    to activate SA signaling, a key defense response in plants.\n                    <jats:italic toggle=\"yes\">Antioxid. Redox Signal.<\/jats:italic>\n                    18, 2106\u20132121.\n                  <\/jats:p>","DOI":"10.1089\/ars.2012.5052","type":"journal-article","created":{"date-parts":[[2012,11,13]],"date-time":"2012-11-13T10:01:30Z","timestamp":1352800890000},"page":"2106-2121","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":243,"title":["Functional Analysis of Arabidopsis Mutants Points to Novel Roles for Glutathione in Coupling H\n                    <sub>2<\/sub>\n                    O\n                    <sub>2<\/sub>\n                    to Activation of Salicylic Acid Accumulation and Signaling"],"prefix":"10.1177","volume":"18","author":[{"given":"Yi","family":"Han","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Paris Sud","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sejir","family":"Chaouch","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Paris Sud","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amna","family":"Mhamdi","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Paris Sud","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume","family":"Queval","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Paris Sud","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernd","family":"Zechmann","sequence":"additional","affiliation":[{"name":"University of Graz","place":["Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graham","family":"Noctor","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Paris 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