{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:20:01Z","timestamp":1791274801777,"version":"4.1.0"},"reference-count":84,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2014,3,1]],"date-time":"2014-03-01T00:00:00Z","timestamp":1393632000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Antioxidants &amp; Redox Signaling"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:p>\n                    <jats:bold>\n                      <jats:italic toggle=\"yes\">Aims:<\/jats:italic>\n                    <\/jats:bold>\n                    An abnormal generation of reactive oxygen species (ROS) is thought to contribute to systemic sclerosis (SSc), fostering autoimmunity, fibrosis, and vascular inflammation. The function of the prototypic damage-associated molecular pattern, high mobility group box 1 (HMGB1), depends on its redox status. Here we investigate whether oxidative stress regulates the cross-talk between leukocytes and platelets\n                    <jats:italic toggle=\"yes\">via<\/jats:italic>\n                    HMGB1, thus contributing to vessel inflammation in SSc.\n                    <jats:bold>\n                      <jats:italic toggle=\"yes\">Results:<\/jats:italic>\n                    <\/jats:bold>\n                    The oxidation of HMGB1 amplified its ability to activate neutrophils, as detected assessing the redistribution of primary granule molecules and the transactivation of the \u03b22 integrin chain CD18. Activated platelets are a source of bioactive HMGB1 and\n                    <jats:italic toggle=\"yes\">via<\/jats:italic>\n                    P-selectin stimulated neutrophils to generate ROS. Oxidized extracellular HMGB1, soluble or associated to platelet membrane or to platelet-derived microparticles (PD\u03bcPs), further increased leukocyte activation. Leukocyte activation abated in the presence of inhibitors of HMGB1 or of catalase, which catalyzes the dismutation of hydrogen peroxide into water and molecular oxygen. The redistribution of the content of primary granules and the transactivation of \u03b22 integrins characterized blood leukocytes of SSc patients and membrane HMGB1 was significantly higher in patients with pulmonary hypertension or with diffuse SSc. HMGB1\n                    <jats:sup>+<\/jats:sup>\n                    microparticles (\u03bcPs) purified from SSc patients, but not HMGB1\n                    <jats:sup>\u2212<\/jats:sup>\n                    \u03bcPs purified from control subjects, activated\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    healthy neutrophils, and HMGB1 inhibitors reversed the effects of \u03bcPs.\n                    <jats:bold>\n                      <jats:italic toggle=\"yes\">Innovation and Conclusion:<\/jats:italic>\n                    <\/jats:bold>\n                    ROS dramatically increase the ability of extracellular HMGB1 to activate blood leukocytes. This event might contribute to maintain the microvascular injury of patients with SSc.\n                    <jats:italic toggle=\"yes\">Antioxid. Redox Signal.<\/jats:italic>\n                    20, 1060\u20131074.\n                  <\/jats:p>","DOI":"10.1089\/ars.2013.5298","type":"journal-article","created":{"date-parts":[[2013,9,26]],"date-time":"2013-09-26T12:57:05Z","timestamp":1380200225000},"page":"1060-1074","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":95,"title":["Oxidative Stress Elicits Platelet\/Leukocyte Inflammatory Interactions\n                    <i>via<\/i>\n                    HMGB1: A Candidate for Microvessel Injury in Sytemic Sclerosis"],"prefix":"10.1177","volume":"20","author":[{"given":"Norma","family":"Maugeri","sequence":"first","affiliation":[{"name":"San Raffaele Scientific Institute","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrizia","family":"Rovere-Querini","sequence":"additional","affiliation":[{"name":"San Raffaele Scientific Institute","place":["Italy"]},{"name":"Vita-Salute San Raffaele University","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mattia","family":"Baldini","sequence":"additional","affiliation":[{"name":"San Raffaele Scientific Institute","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Baldissera","sequence":"additional","affiliation":[{"name":"San Raffaele Scientific Institute","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Grazia","family":"Sabbadini","sequence":"additional","affiliation":[{"name":"San Raffaele Scientific Institute","place":["Italy"]},{"name":"Vita-Salute San Raffaele University","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco E.","family":"Bianchi","sequence":"additional","affiliation":[{"name":"Vita-Salute San Raffaele University","place":["Italy"]},{"name":"San Raffaele Scientific Institute","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelo A.","family":"Manfredi","sequence":"additional","affiliation":[{"name":"San Raffaele Scientific Institute","place":["Italy"]},{"name":"Vita-Salute San Raffaele University","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2014,3,1]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-030409-101323"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3899\/jrheum.100398"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V96.9.3070"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/ker520"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2010.133587"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.2119\/molmed.2012.00283"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.11.6113"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2011.149"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1172794"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0908576"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2012.5164"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-010-1033-1"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1053\/sarh.2002.50011"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI62423"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2376"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20101629"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.30361"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20100561"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.12.7506"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1160\/TH05-01-0020"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-06-029082"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rdc.2008.01.001"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1201951"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra0806188"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-008-0125-4"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v17.i36.4090"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.23735"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/ken267"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2007-0330OC"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2011.222"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.12120"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1121146109"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2012.96"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2006.07.020"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2013.5179"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rdc.2007.12.004"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2008.05.013"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0005704"},{"key":"e_1_3_3_40_2","first-page":"202","article-title":"Scleroderma (systemic sclerosis): classification, subsets and pathogenesis","volume":"15","author":"LeRoy EC","year":"1988","unstructured":"LeRoy EC, , Black C, , Fleischmajer R, , Jablonska S, , Krieg T, , Medsger TAJr, ., Rowell N, , and Wollheim F. Scleroderma (systemic sclerosis): classification, subsets and pathogenesis. J Rheumatol, 15: 202\u2013205, 1988.","journal-title":"J Rheumatol"},{"key":"e_1_3_3_41_2","first-page":"1573","article-title":"Criteria for the classification of early systemic sclerosis","volume":"28","author":"LeRoy EC","year":"2001","unstructured":"LeRoy EC, and Medsger TAJr. Criteria for the classification of early systemic sclerosis. J Rheumatol, 28: 1573\u20131576, 2001.","journal-title":"J Rheumatol"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.111.242057"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.10-159921"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1080\/08916930902832629"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.3109\/08916934.2012.719946"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-02-337337"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-09-180794"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0039484"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1100261"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.112.3.491"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/0190-9622(93)70014-K"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.3899\/jrheum.100780"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.3109\/09537104.2012.719090"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2012.5143"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2010.3865"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kes028"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1097\/BOR.0b013e3282eeb3a4"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2012.00160"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12013-010-9077-0"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2010.3363"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1097\/00002281-199711000-00010"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2008.01.009"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0037-1614175"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.embor.7400205"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2007.08.004"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2009.2377"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11926-012-0296-9"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20111739"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2010.090763"},{"key":"e_1_3_3_70_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kep154"},{"key":"e_1_3_3_71_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2010.3356"},{"key":"e_1_3_3_72_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20051924"},{"key":"e_1_3_3_73_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2010.102"},{"key":"e_1_3_3_74_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)82460-7"},{"key":"e_1_3_3_75_2","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2012.49"},{"key":"e_1_3_3_76_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20120189"},{"key":"e_1_3_3_77_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2012.10.037"},{"key":"e_1_3_3_78_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2010.05748.x"},{"key":"e_1_3_3_79_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2010.3341"},{"key":"e_1_3_3_80_2","doi-asserted-by":"publisher","DOI":"10.1186\/ar3450"},{"key":"e_1_3_3_81_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12016-008-8106-x"},{"key":"e_1_3_3_82_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1003893107"},{"key":"e_1_3_3_83_2","doi-asserted-by":"publisher","DOI":"10.2119\/molmed.2011.00389"},{"key":"e_1_3_3_84_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10875-008-9252-x"},{"key":"e_1_3_3_85_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-011-0835-2"}],"container-title":["Antioxidants &amp; Redox Signaling"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/ars.2013.5298","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/ars.2013.5298","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/ars.2013.5298","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T11:53:13Z","timestamp":1777377193000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/ars.2013.5298"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,3]]},"references-count":84,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2014,3]]}},"alternative-id":["10.1089\/ars.2013.5298"],"URL":"https:\/\/doi.org\/10.1089\/ars.2013.5298","relation":{},"ISSN":["1523-0864","1557-7716"],"issn-type":[{"value":"1523-0864","type":"print"},{"value":"1557-7716","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,3]]}}}