{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T01:46:05Z","timestamp":1790819165091,"version":"4.1.0"},"reference-count":43,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Antioxidants &amp; Redox Signaling"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:p>\n                    <jats:bold>\n                      <jats:italic toggle=\"yes\">Aims:<\/jats:italic>\n                    <\/jats:bold>\n                    A three-step catalytic cycle is common to all peroxiredoxins (Prxs), despite structural and kinetic differences. The second step in 1-Cys type Prxs is a matter of debate since they lack an additional cysteine to play the resolving role, as happens with the 2-Cys Prxs. The aim of this study was to elucidate the role of glutathione (GSH) in the thioredoxin-dependent peroxidase activity of\n                    <jats:italic toggle=\"yes\">Saccharomyces cerevisiae<\/jats:italic>\n                    mitochondrial Prx1p, a 1-Cys type Prx.\n                    <jats:bold>\n                      <jats:italic toggle=\"yes\">Results:<\/jats:italic>\n                    <\/jats:bold>\n                    The peroxidatic Cys91 residue of two Prx1p peptides can be linked by a disulfide, which can be reduced by thioredoxin and by GSH (Km=6.1\u2009\u03bc\n                    <jats:italic toggle=\"yes\">M<\/jats:italic>\n                    ). GSH forms a mixed disulfide with the peroxidatic cysteine spontaneously\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    . Mitochondrial Trx3p deglutathionylates Prx1p without formation of GSSG so that GSH is not consumed in the process. The structural unit of native Prx1p is a dimer whose subunits are not covalently linked, but a hexameric assembly of three disulfide-bound dimers can also be formed.\n                    <jats:bold>\n                      <jats:italic toggle=\"yes\">Innovation:<\/jats:italic>\n                    <\/jats:bold>\n                    GSH is presented as a protective cofactor of Prx1p, which is not consumed during the peroxidase reaction, but provides a robust mechanism as the resolving cysteine and efficiently prevents Prx1p overoxidation. GSH exerts these roles at concentrations well below those commonly considered necessary for its antioxidant and redox buffering functions.\n                    <jats:bold>\n                      <jats:italic toggle=\"yes\">Conclusion:<\/jats:italic>\n                    <\/jats:bold>\n                    A 1-Cys peroxide scavenging mechanism operates in yeast mitochondria involving an autonomous glutathione molecule and the thioredoxin system, which could have universal validity. Prx1p is fairly well protected from overoxidation, questioning its role in a floodgate mechanism for H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    signaling.\n                    <jats:italic toggle=\"yes\">Antioxid. Redox Signal.<\/jats:italic>\n                    24, 115\u2013128.\n                  <\/jats:p>","DOI":"10.1089\/ars.2015.6366","type":"journal-article","created":{"date-parts":[[2015,7,10]],"date-time":"2015-07-10T07:59:24Z","timestamp":1436515164000},"page":"115-128","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":38,"title":["Glutathione Is the Resolving Thiol for Thioredoxin Peroxidase Activity of 1-Cys Peroxiredoxin Without Being Consumed During the Catalytic Cycle"],"prefix":"10.1177","volume":"24","author":[{"given":"Jos\u00e9 Rafael","family":"Pedrajas","sequence":"first","affiliation":[{"name":"University of Ja\u00e9n","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"McDonagh","sequence":"additional","affiliation":[{"name":"University of Liverpool","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Hern\u00e1ndez-Torres","sequence":"additional","affiliation":[{"name":"University of Ja\u00e9n","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Miranda-Vizuete","sequence":"additional","affiliation":[{"name":"Hospital Universitario Virgen del Roc\u00edo\/CSIC\/Universidad de Sevilla","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ra\u00fal","family":"Gonz\u00e1lez-Ojeda","sequence":"additional","affiliation":[{"name":"University of C\u00f3rdoba","place":["Spain"]},{"name":"C\u00f3rdoba Maim\u00f3nides Institute for Biomedical Research","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilia","family":"Mart\u00ednez-Galisteo","sequence":"additional","affiliation":[{"name":"University of C\u00f3rdoba","place":["Spain"]},{"name":"C\u00f3rdoba Maim\u00f3nides Institute for Biomedical Research","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. Alicia","family":"Padilla","sequence":"additional","affiliation":[{"name":"University of C\u00f3rdoba","place":["Spain"]},{"name":"C\u00f3rdoba Maim\u00f3nides Institute for Biomedical Research","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Antonio","family":"B\u00e1rcena","sequence":"additional","affiliation":[{"name":"University of C\u00f3rdoba","place":["Spain"]},{"name":"C\u00f3rdoba Maim\u00f3nides Institute for Biomedical Research","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2016,1,20]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20120505"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1158265"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq662"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku340"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2013.02.017"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2014.5998"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.56.032604.144246"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2011.4260"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.112.197723"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsb0598-400"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20090242"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/tx100413v"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.111.016162"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01918-08"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2012.4597"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2091-11-3"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2009.06985.x"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2010.3624"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1042\/BST0331375"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2004.05.002"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.4.2924"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2008.04.101"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2011.105"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0400181101"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0700481104"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)84088-7"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi101373h"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.10.6366"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.21.16296"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2009.2950"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj20020570"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2008.02.043"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.19.12.8180"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2010.5"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M112.017707"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3600001"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq1060"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.015487"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.108373"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.07.045"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1080405"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3213"}],"container-title":["Antioxidants &amp; 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