{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:13:19Z","timestamp":1790737999647,"version":"4.1.0"},"reference-count":89,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Antioxidants &amp; Redox Signaling"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:sec>\n                    <jats:title>Aims:<\/jats:title>\n                    <jats:p>\n                      Cysteine (Cys) is a major target for redox post-translational modifications (PTMs) that occur in response to changes in the cellular redox environment. We describe multiplexed, peptide-based enrichment and quantitative mass spectrometry (MS) applied to globally profile reversible redox Cys PTM in rat hearts during ischemia\/reperfusion (I\/R) in the presence or absence of an aminothiol antioxidant,\n                      <jats:italic toggle=\"yes\">N<\/jats:italic>\n                      -2-mercaptopropionylglycine (MPG). Parallel fractionation also allowed identification of irreversibly oxidized Cys peptides (Cys-SO\n                      <jats:sub>2<\/jats:sub>\n                      H\/SO\n                      <jats:sub>3<\/jats:sub>\n                      H).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      We identified 4505 reversibly oxidized Cys peptides of which 1372 were significantly regulated by ischemia and\/or I\/R. An additional 219 peptides (247 sites) contained Cys-SO\n                      <jats:sub>2<\/jats:sub>\n                      H\/Cys-SO\n                      <jats:sub>3<\/jats:sub>\n                      H modifications, and these were predominantly identified from hearts subjected to I\/R (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      \u2009=\u2009168 peptides). Parallel reaction monitoring MS (PRM-MS) enabled relative quantitation of 34 irreversibly oxidized Cys peptides. MPG attenuated a large cluster of I\/R-associated reversibly oxidized Cys peptides and irreversible Cys oxidation to less than nonischemic controls (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      \u2009=\u200924 and 34 peptides, respectively). PRM-MS showed that Cys sites oxidized during ischemia and\/or I\/R and \u201cprotected\u201d by MPG were largely mitochondrial, and were associated with antioxidant functions (peroxiredoxins 5 and 6) and metabolic processes, including glycolysis. Metabolomics revealed I\/R induced changes in glycolytic intermediates that were reversed in the presence of MPG, which were consistent with irreversible PTM of triose phosphate isomerase and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), altered GAPDH enzyme activity, and reduced I\/R glycolytic payoff as evidenced by adenosine triphosphate and NADH levels.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Innovation:<\/jats:title>\n                    <jats:p>Novel enrichment and PRM-MS approaches developed here enabled large-scale relative quantitation of Cys redox sites modified by reversible and irreversible PTM during I\/R and antioxidant remediation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>Cys sites identified here are targets of reactive oxygen species that can contribute to protein dysfunction and the pathogenesis of I\/R.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/ars.2019.7765","type":"journal-article","created":{"date-parts":[[2020,7,30]],"date-time":"2020-07-30T05:13:51Z","timestamp":1596086031000},"page":"11-31","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":38,"title":["A Global Profile of Reversible and Irreversible Cysteine Redox Post-Translational Modifications During Myocardial Ischemia\/Reperfusion Injury and Antioxidant Intervention"],"prefix":"10.1177","volume":"34","author":[{"given":"Alexander W.","family":"Rookyard","sequence":"first","affiliation":[{"name":"School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia."},{"name":"Charles Perkins Centre, The University of Sydney, Sydney, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"Paulech","sequence":"additional","affiliation":[{"name":"School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stine","family":"Thyssen","sequence":"additional","affiliation":[{"name":"School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia."},{"name":"Charles Perkins Centre, The University of Sydney, Sydney, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiersten A.","family":"Liddy","sequence":"additional","affiliation":[{"name":"School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia."},{"name":"Charles Perkins Centre, The University of Sydney, Sydney, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Max","family":"Puckeridge","sequence":"additional","affiliation":[{"name":"School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Desmond K.","family":"Li","sequence":"additional","affiliation":[{"name":"Charles Perkins Centre, The University of Sydney, Sydney, Australia."},{"name":"Discipline of Pathology, School of Medical Sciences, The University of Sydney, Sydney, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melanie Y.","family":"White","sequence":"additional","affiliation":[{"name":"School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia."},{"name":"Charles Perkins Centre, The University of Sydney, Sydney, Australia."},{"name":"Discipline of Pathology, School of Medical Sciences, The University of Sydney, Sydney, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart J.","family":"Cordwell","sequence":"additional","affiliation":[{"name":"School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia."},{"name":"Charles Perkins Centre, The University of Sydney, Sydney, Australia."},{"name":"Discipline of Pathology, School of Medical Sciences, The University of Sydney, Sydney, Australia."},{"name":"Sydney Mass Spectrometry, The University of Sydney, Sydney, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2020,12,1]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1211485"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2003.10.025"},{"key":"e_1_3_4_4_2","first-page":"H2240","article-title":"Generation of superoxide in cardiomyocytes during ischemia before reperfusion","volume":"277","author":"Becker LB","year":"1999","unstructured":"Becker LB, , Vanden Hoek TL, , Shao ZH, , Li CQ, , and Schumacker PT. 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