{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:12:19Z","timestamp":1785888739298,"version":"3.56.0"},"reference-count":49,"publisher":"SAGE Publications","issue":"4-6","license":[{"start":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T00:00:00Z","timestamp":1693872000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Antioxidants &amp; Redox Signaling"],"published-print":{"date-parts":[[2026,8,1]]},"abstract":"<jats:sec>\n                    <jats:title>Aims:<\/jats:title>\n                    <jats:p>\n                      Cystathionine \u03b2-synthase (CBS) is essential for homocysteine (Hcy) transsulfuration, yielding cysteine as a common precursor of hydrogen sulfide (H\n                      <jats:sub>2<\/jats:sub>\n                      S), glutathione (GSH), and other sulfur molecules, which produce neuroprotective effects in neurological conditions. We previously reported a disruption of microglial CBS\/H\n                      <jats:sub>2<\/jats:sub>\n                      S signaling in a Parkinson\u2019s disease (PD) mouse model. Yet, it remains unclear whether CBS affects nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain-containing 3 (NLRP3) inflammasome activity and other pathologies in PD.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Microglial CBS expression decreased after lipopolysaccharide (LPS) stimulation. Elevated GSSG (the oxidized GSH) content and decreased H\n                      <jats:sub>2<\/jats:sub>\n                      S generation were found in the brains of microglial\n                      <jats:italic toggle=\"yes\">cbs<\/jats:italic>\n                      conditional-knockout (\n                      <jats:italic toggle=\"yes\">cbs<\/jats:italic>\n                      <jats:sup>cKO<\/jats:sup>\n                      ) mice, whereas serum and brain Hcy levels remained unaltered. Moreover, microglial\n                      <jats:italic toggle=\"yes\">cbs<\/jats:italic>\n                      <jats:sup>cKO<\/jats:sup>\n                      mice were susceptible to NLRP3 inflammasome activation and dopaminergic neuron losses caused by LPS injection into the substantia nigra, whereas\n                      <jats:italic toggle=\"yes\">cbs<\/jats:italic>\n                      overexpression or activation produced opposite effects.\n                      <jats:italic toggle=\"yes\">In vitro<\/jats:italic>\n                      studies showed that\n                      <jats:italic toggle=\"yes\">cbs<\/jats:italic>\n                      overexpression or activation suppressed microglial NLRP3 inflammasome activation and interleukin (IL)-1\u03b2 secretion by reducing mitochondrial reactive oxygen species (mitoROS) level. Conversely, ablation of\n                      <jats:italic toggle=\"yes\">cbs<\/jats:italic>\n                      enhanced NLRP3 expression and mitoROS generation and augmented microglial NLRP3 inflammasome activity in response to adenosine triphosphate challenge, which was blocked by the mitoROS scavenger.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Innovation and Conclusion:<\/jats:title>\n                    <jats:p>\n                      The study demonstrated an elevated GSSG level and reduced H\n                      <jats:sub>2<\/jats:sub>\n                      S generation, which correlated with a susceptible status of microglia in the brain of\n                      <jats:italic toggle=\"yes\">cbs<\/jats:italic>\n                      <jats:sup>cKO<\/jats:sup>\n                      mice. Our findings reveal a critical role of CBS in restraining the microglial NLRP3 inflammasome by controlling redox homeostasis and highlight that activation or upregulation of CBS may become a potential strategy for PD treatment.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/ars.2022.0174","type":"journal-article","created":{"date-parts":[[2023,7,19]],"date-time":"2023-07-19T02:12:57Z","timestamp":1689732777000},"page":"198-218","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":13,"title":["Cystathionine \u03b2-Synthase Suppresses NLRP3 Inflammasome Activation\n                    <i>via<\/i>\n                    Redox Regulation in Microglia"],"prefix":"10.1177","volume":"45","author":[{"given":"Yu-Jie","family":"Mou","sequence":"first","affiliation":[{"name":"Department of Neurology and Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou, China."},{"name":"Department of Pharmacology, College of Pharmaceutical Sciences, Soochow University, Suzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Ting","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Neurology and Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou, China."},{"name":"Jiangsu Key Laboratory of Neuropsychiatric Diseases and Institute of Neuroscience, Soochow University, Suzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Yuan","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Neuropsychiatric Diseases and Institute of Neuroscience, Soochow University, Suzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurology and Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou, China."},{"name":"Jiangsu Key Laboratory of Neuropsychiatric Diseases and Institute of Neuroscience, Soochow University, Suzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Neurology and Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou, China."},{"name":"Jiangsu Key Laboratory of Neuropsychiatric Diseases and Institute of Neuroscience, Soochow University, Suzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chong-Shuang","family":"Pei","sequence":"additional","affiliation":[{"name":"Department of Neurology and Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou, China."},{"name":"Jiangsu Key Laboratory of Neuropsychiatric Diseases and Institute of Neuroscience, Soochow University, Suzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun-Feng","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Neurology and Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou, China."},{"name":"Jiangsu Key Laboratory of Neuropsychiatric Diseases and Institute of Neuroscience, Soochow University, Suzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Ou","family":"Hou","sequence":"additional","affiliation":[{"name":"Department of Neurology and Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou, China."},{"name":"Jiangsu Key Laboratory of Neuropsychiatric Diseases and Institute of Neuroscience, Soochow University, Suzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8326-7779","authenticated-orcid":false,"given":"Li-Fang","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Neurology and Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou, China."},{"name":"Jiangsu Key Laboratory of Neuropsychiatric Diseases and Institute of Neuroscience, Soochow University, Suzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2023,9,5]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja404004v"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2018.07.014"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00702-004-0222-z"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M117.806869"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.00936"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0761-3"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2014.07.107"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1023\/a:1024255208563"},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.05-3724fje"},{"key":"e_1_3_3_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aah4066"},{"key":"e_1_3_3_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2021.102010"},{"key":"e_1_3_3_13_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/6137521"},{"key":"e_1_3_3_14_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1202737"},{"key":"e_1_3_3_15_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2010.3517"},{"key":"e_1_3_3_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1474-9726.2009.00543.x"},{"key":"e_1_3_3_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2006.04283.x"},{"key":"e_1_3_3_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2019.101223"},{"key":"e_1_3_3_19_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2005.7.813"},{"key":"e_1_3_3_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aaz5752"},{"key":"e_1_3_3_21_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2010.3671"},{"key":"e_1_3_3_22_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2018.3993"},{"key":"e_1_3_3_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-018-0124-5"},{"key":"e_1_3_3_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-017-2185-0"},{"key":"e_1_3_3_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.2000464"},{"key":"e_1_3_3_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.752482"},{"key":"e_1_3_3_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2022.102233"},{"key":"e_1_3_3_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-016-9980-5"},{"key":"e_1_3_3_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-018-1236-z"},{"key":"e_1_3_3_30_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.02.034"},{"key":"e_1_3_3_31_1","doi-asserted-by":"publisher","DOI":"10.1177\/002215540305100311"},{"key":"e_1_3_3_32_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2013.5745"},{"key":"e_1_3_3_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja207851s"},{"key":"e_1_3_3_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14446"},{"key":"e_1_3_3_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2019.00349"},{"key":"e_1_3_3_36_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2008.2253"},{"key":"e_1_3_3_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23115938"},{"key":"e_1_3_3_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/acel.13522"},{"key":"e_1_3_3_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms2623"},{"key":"e_1_3_3_40_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00036.2004"},{"key":"e_1_3_3_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41531-018-0061-5"},{"key":"e_1_3_3_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2022.102442"},{"key":"e_1_3_3_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.11.047"},{"key":"e_1_3_3_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2017.07.159"},{"key":"e_1_3_3_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-013-0969-4"},{"key":"e_1_3_3_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2017.07.156"},{"key":"e_1_3_3_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/mds.26488"},{"key":"e_1_3_3_48_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-017-0940-4"},{"key":"e_1_3_3_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09663"},{"key":"e_1_3_3_50_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13024-016-0094-3"}],"container-title":["Antioxidants &amp; Redox Signaling"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/ars.2022.0174","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/ars.2022.0174","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/ars.2022.0174","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T15:09:33Z","timestamp":1782918573000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/ars.2022.0174"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,5]]},"references-count":49,"journal-issue":{"issue":"4-6","published-print":{"date-parts":[[2026,8,1]]}},"alternative-id":["10.1089\/ars.2022.0174"],"URL":"https:\/\/doi.org\/10.1089\/ars.2022.0174","relation":{},"ISSN":["1523-0864","1557-7716"],"issn-type":[{"value":"1523-0864","type":"print"},{"value":"1557-7716","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,5]]}}}