{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T06:29:38Z","timestamp":1792045778756,"version":"4.4.0"},"reference-count":11,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2023,5,22]],"date-time":"2023-05-22T00:00:00Z","timestamp":1684713600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,5,22]],"date-time":"2023-05-22T00:00:00Z","timestamp":1684713600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Astrobiology"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:p>\n                    With the imminent human exploration of deep space, it is more important than ever to understand the biological risks of deep space radiation exposure. The BioSentinel mission will be the first biological payload to study the effects of radiation beyond low Earth orbit in 50 years. This study is the last in a collection of articles about the BioSentinel biological CubeSat mission, where budding yeast cells will be used to investigate the response of a biological organism to long-term, low-dose deep space radiation. In this study, we define the methodology for detecting the biological response to space-like radiation using simulated deep space radiation and a metabolic indicator dye reduction assay. We show that there is a dose-dependent decrease in yeast cell growth and metabolism in response to space-like radiation, and this effect is significantly more pronounced in a strain of yeast that is deficient in DNA damage repair (\n                    <jats:italic toggle=\"yes\">rad51<\/jats:italic>\n                    \u0394) compared with a wild-type strain. Furthermore, we demonstrate the use of flight-like instrumentation after exposure to space-like ionizing radiation. Our findings will inform the development of novel and improved biosensors and technologies for future missions to deep space.\n                  <\/jats:p>","DOI":"10.1089\/ast.2022.0124","type":"journal-article","created":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T10:03:55Z","timestamp":1681380235000},"page":"648-656","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":17,"title":["BioSentinel: Validating Sensitivity of Yeast Biosensors to Deep Space Relevant Radiation"],"prefix":"10.1177","volume":"23","author":[{"given":"Lauren C.","family":"Liddell","sequence":"first","affiliation":[{"name":"NASA Ames Research Center, Moffett Field, California, USA."},{"name":"Logyx LLC, Mountain View, California, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana M.","family":"Gentry","sequence":"additional","affiliation":[{"name":"NASA Ames Research Center, Moffett Field, California, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel","family":"Gilbert","sequence":"additional","affiliation":[{"name":"NASA Ames Research Center, Moffett Field, California, USA."},{"name":"FILMSS\/KBR, NASA Ames Research Center, Moffett Field, California, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Marina","sequence":"additional","affiliation":[{"name":"Amyris, Inc., Emeryville, California, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofia","family":"Massaro Tieze","sequence":"additional","affiliation":[{"name":"Yale School of Medicine, New Haven, Connecticut, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5952-3728","authenticated-orcid":false,"given":"Michael R.","family":"Padgen","sequence":"additional","affiliation":[{"name":"NASA Ames Research Center, Moffett Field, California, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kylie","family":"Akiyama","sequence":"additional","affiliation":[{"name":"Space Life Sciences Training Program, NASA Ames Research Center, Moffett Field, California, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyra","family":"Keenan","sequence":"additional","affiliation":[{"name":"Space Life Sciences Training Program, NASA Ames Research Center, Moffett Field, California, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1562-2692","authenticated-orcid":false,"given":"Sharmila","family":"Bhattacharya","sequence":"additional","affiliation":[{"name":"NASA Ames Research Center, Moffett Field, California, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1300-4329","authenticated-orcid":false,"given":"Sergio R.","family":"Santa Maria","sequence":"additional","affiliation":[{"name":"NASA Ames Research Center, Moffett Field, California, USA."},{"name":"FILMSS\/KBR, NASA Ames Research Center, Moffett Field, California, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2023,5,22]]},"reference":[{"key":"e_1_3_5_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/life4030491"},{"key":"e_1_3_5_3_1","author":"Coutinho C","year":"1988","unstructured":"Coutinho C, , Bernardes E, , Felix D, et al. Trehalose as cryoprotectant for preservation of yeast strains. J Biotechnol, 1988:7(1);23\u201332; https:\/\/doi.org\/10.1016\/0168-1656(88)90032-6","journal-title":"J Biotechnol"},{"key":"e_1_3_5_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa0769"},{"key":"e_1_3_5_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/01926230290166724"},{"key":"e_1_3_5_6_1","article-title":"BioSentinel: A biological CubeSat for deep space exploration","author":"Massaro Tieze S","year":"2020","unstructured":"Massaro Tieze S, , Liddell LC, , Santa Maria SR, et al. BioSentinel: A biological CubeSat for deep space exploration. Astrobiology, 2020; https:\/\/doi.org\/10.1089\/ast.2019.2068","journal-title":"Astrobiology"},{"key":"e_1_3_5_7_1","article-title":"BioSentinel: A biofluidic nanosatellite monitoring microbial growth and activity in deep space","author":"Padgen MR","year":"2021","unstructured":"Padgen MR, , Liddell LC, , Bhardwaj SR, et al. BioSentinel: A biofluidic nanosatellite monitoring microbial growth and activity in deep space. Astrobiology, 2021; http:\/\/doi.org\/10.1089\/ast.2020.2305","journal-title":"Astrobiology"},{"key":"e_1_3_5_8_1","doi-asserted-by":"publisher","DOI":"10.1109\/MAES.2019.2953760"},{"key":"e_1_3_5_9_1","article-title":"BioSentinel: Long-term Saccharomyces cerevisiae preservation for a deep space biosensor mission","author":"Santa Maria SR","year":"2020","unstructured":"Santa Maria SR, , Marina D, , Massaro Tieze S, et al. BioSentinel: Long-term Saccharomyces cerevisiae preservation for a deep space biosensor mission. Astrobiology, 2020; https:\/\/doi.org\/10.1089\/ast.2019.2073","journal-title":"Astrobiology"},{"key":"e_1_3_5_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000669"},{"key":"e_1_3_5_11_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:CANC.0000031769.14728.bc"},{"key":"e_1_3_5_12_1","doi-asserted-by":"publisher","DOI":"10.14338\/IJPT-18-00013.1"}],"container-title":["Astrobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/ast.2022.0124","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/ast.2022.0124","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/ast.2022.0124","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T12:04:44Z","timestamp":1777377884000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/ast.2022.0124"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,22]]},"references-count":11,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,6]]}},"alternative-id":["10.1089\/ast.2022.0124"],"URL":"https:\/\/doi.org\/10.1089\/ast.2022.0124","relation":{},"ISSN":["1531-1074","1557-8070"],"issn-type":[{"value":"1531-1074","type":"print"},{"value":"1557-8070","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,22]]}}}