{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T23:36:48Z","timestamp":1792021008341,"version":"4.4.0"},"reference-count":70,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T00:00:00Z","timestamp":1722470400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T00:00:00Z","timestamp":1722470400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Astrobiology"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:p>\n                    The study of extremophilic microorganisms has sparked interest in understanding extraterrestrial microbial life. Such organisms are fundamental for investigating life forms on Saturn\u2019s icy moons, such as Enceladus, which is characterized by potentially habitable saline and alkaline niches. Our study focused on the salt-alkaline soil of the Al Wahbah crater in Saudi Arabia, where we identified microorganisms that could be used as biological models to understand potential life on Enceladus. The search involved isolating 48 bacterial strains, sequencing the genomes of two thermo-haloalkaliphilic strains, and characterizing them for astrobiological application. A deeper understanding of the genetic composition and functional capabilities of the two novel strains of\n                    <jats:italic toggle=\"yes\">Halalkalibacterium halodurans<\/jats:italic>\n                    provided valuable insights into their survival strategies and the presence of coding genes and pathways related to adaptations to environmental stressors. We also used mass spectrometry with a molecular network approach, highlighting various classes of molecules, such as phospholipids and nonproteinogenic amino acids, as potential biosignatures. These are essential features for understanding life\u2019s adaptability under extreme conditions and could be used as targets for biosignatures in upcoming missions exploring Enceladus\u2019 orbit. Furthermore, our study reinforces the need to look at new extreme environments on Earth that might contribute to the astrobiology field.\n                  <\/jats:p>","DOI":"10.1089\/ast.2024.0017","type":"journal-article","created":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T16:40:41Z","timestamp":1724085641000},"page":"824-838","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":11,"title":["Investigating Polyextremophilic Bacteria in Al Wahbah Crater, Saudi Arabia: A Terrestrial Model for Life on Saturn\u2019s Moon Enceladus"],"prefix":"10.1177","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5814-3624","authenticated-orcid":false,"given":"Alef","family":"dos Santos","sequence":"first","affiliation":[{"name":"Department of Chemistry, Federal University of S\u00e3o Carlos, S\u00e3o Carlos, Brazil."},{"name":"Bioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00fania","family":"Schultz","sequence":"additional","affiliation":[{"name":"Bioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marilia","family":"Almeida Trapp","sequence":"additional","affiliation":[{"name":"Analytical Core Laboratory, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fluvio","family":"Modolon","sequence":"additional","affiliation":[{"name":"Bioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia."},{"name":"Laboratory of Molecular Microbial Ecology, Institute of Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrii","family":"Romanenko","sequence":"additional","affiliation":[{"name":"Bioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arun","family":"Kumar Jaiswal","sequence":"additional","affiliation":[{"name":"Post-Graduate Program in Bioinformatics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas","family":"Gomes","sequence":"additional","affiliation":[{"name":"Post-Graduate Program in Bioinformatics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edson","family":"Rodrigues-Filho","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Federal University of S\u00e3o Carlos, S\u00e3o Carlos, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5135-1394","authenticated-orcid":false,"given":"Alexandre Soares","family":"Rosado","sequence":"additional","affiliation":[{"name":"Bioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,8,19]]},"reference":[{"key":"e_1_3_6_2_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1473550417000295"},{"key":"e_1_3_6_3_1","unstructured":"Andrews S. 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