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Although fully automated or semiautomated technologies for TMA creation provide excellent precision with respect to core transfer, they do not obviate the need for technical expertise to successfully generate high-quality TMA blocks and derivative sections. We have leveraged our expanding bank of formalin-fixed paraffin embedded paired tumor and normal tissues in our academic cancer center to provide a rich source of input material for cancer research TMAs. In this study, we report a stepwise optimization of TMA generation parameters, including paraffin wax selection, tempering protocol, and sectioning conditions, to achieve the best ribbon sectioning.<\/jats:p>","DOI":"10.1089\/bio.2019.0011","type":"journal-article","created":{"date-parts":[[2019,6,13]],"date-time":"2019-06-13T15:03:48Z","timestamp":1560438228000},"page":"452-457","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Optimization of Tissue Microarrays from Banked Human Formalin-Fixed Paraffin Embedded Tissues in the Cancer Research Setting"],"prefix":"10.1177","volume":"17","author":[{"given":"Tammy","family":"Sexton","sequence":"first","affiliation":[{"name":"Wake Forest Baptist Comprehensive Cancer Center, Wake Forest School of Medicine, Winston-Salem, North Carolina."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory L.","family":"Kucera","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Section on Hematology and Oncology, Wake Forest School of Medicine, Winston-Salem, North Carolina."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward A.","family":"Levine","sequence":"additional","affiliation":[{"name":"Surgical Oncology Service, Department of General Surgery, Wake Forest School of Medicine, Winston-Salem, North Carolina."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kounosuke","family":"Watabe","sequence":"additional","affiliation":[{"name":"Department of Cancer Biology, Wake Forest School of Medicine, Winston-Salem, North Carolina."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stacey S.","family":"O'Neill","sequence":"additional","affiliation":[{"name":"Department of Pathology, Wake Forest School of Medicine, Winston-Salem, North Carolina."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2019,10,10]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0798-844"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60327-216-2_13"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2008.17.3567"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/microarrays3020103"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mpdhp.2009.01.010"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2007.00606.x"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0463.2011.02871.x"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2013.608"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2796.2011.02427.x"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1179\/2046023614Y.0000000046"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1136\/mp.56.5.306"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.4132\/KoreanJPathol.2013.47.3.238"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.6A6987.2006"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.4132\/KoreanJPathol.2012.46.6.562"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12894-018-0392-x"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0100469"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.25655"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01578"}],"container-title":["Biopreservation and Biobanking"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/bio.2019.0011","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/bio.2019.0011","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/bio.2019.0011","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T13:31:29Z","timestamp":1777383089000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/bio.2019.0011"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10]]},"references-count":18,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2019,10]]}},"alternative-id":["10.1089\/bio.2019.0011"],"URL":"https:\/\/doi.org\/10.1089\/bio.2019.0011","relation":{},"ISSN":["1947-5535","1947-5543"],"issn-type":[{"value":"1947-5535","type":"print"},{"value":"1947-5543","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,10]]}}}