{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T14:50:31Z","timestamp":1787237431787,"version":"build-2736575974"},"reference-count":47,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2020,9,28]],"date-time":"2020-09-28T00:00:00Z","timestamp":1601251200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Biopreservation and Biobanking"],"published-print":{"date-parts":[[2020,10,1]]},"abstract":"<jats:p>\n                    Maintaining genetic variability is an important part of the conservation of endangered species, so the construction of germplasm banks is essential. Several species of the genus\n                    <jats:italic toggle=\"yes\">Mazama<\/jats:italic>\n                    endure constant pressure in their natural habitat and are threatened with extinction. The correct manipulation and adequacy of the diluents and cryoprotectants must be studied to be successful in the formation of these banks. The purpose of this study was to evaluate the efficiency of three different cryoprotectants in sperm cryopreservation in the species\n                    <jats:italic toggle=\"yes\">Mazama americana<\/jats:italic>\n                    : 6% glycerol (GLY), 3% ethylene glycol (ETG), and 5% dimethylformamide (DMF). Semen was obtained with the lateral deviation of the penis to an artificial vagina. In the pre-freeze and post-thaw periods, motility, vigor, membrane integrity, acrosome integrity, and sperm cell morphology were evaluated for each of the cryoprotectants. Post-thaw motility was higher when semen was frozen with cryoprotectants GLY and DMF (55.31\u2009\u00b1\u20097.39 and 55.94\u2009\u00b1\u20092.77, respectively), compared with the result obtained for ETG (48.13\u2009\u00b1\u20092.39). For major defects (MaD), a difference was observed between the pre- and post-cryopreservation periods, such that DMF generated a higher number of post-thaw MaD (25.94\u2009\u00b1\u20095.37). All cryoprotectants were efficient for cryopreservation of\n                    <jats:italic toggle=\"yes\">M. americana<\/jats:italic>\n                    semen, resulting in samples with satisfactory viability after thawing. However, the medium with the cryoprotectants GLY, at a concentration of 6%, and DMF, at a concentration of 5%, were preferable.\n                  <\/jats:p>","DOI":"10.1089\/bio.2020.0012","type":"journal-article","created":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T11:10:52Z","timestamp":1593601852000},"page":"351-357","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Comparison Between Three Cryoprotectants in the Freezing of\n                    <i>Mazama americana<\/i>\n                    Semen Collected by Artificial Vagina"],"prefix":"10.1177","volume":"18","author":[{"given":"Maria C.L.","family":"Alvarez","sequence":"first","affiliation":[{"name":"N\u00facleo de Pesquisa e Conserva\u00e7\u00e3o de Cerv\u00eddeos (NUPECCE), Departamento de Zootecnia, Faculdade de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias, Universidade Estadual Paulista, Jaboticabal, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana D.","family":"Rola","sequence":"additional","affiliation":[{"name":"N\u00facleo de Pesquisa e Conserva\u00e7\u00e3o de Cerv\u00eddeos (NUPECCE), Departamento de Zootecnia, Faculdade de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias, Universidade Estadual Paulista, Jaboticabal, Brazil."},{"name":"Departamento de Zootecnia, Centro de Ci\u00eancias Agr\u00e1rias\u2014Campus II, Universidade Federal da Para\u00edba, Areia, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 M.B.","family":"Duarte","sequence":"additional","affiliation":[{"name":"N\u00facleo de Pesquisa e Conserva\u00e7\u00e3o de Cerv\u00eddeos (NUPECCE), Departamento de Zootecnia, Faculdade de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias, Universidade Estadual Paulista, Jaboticabal, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2020,9,28]]},"reference":[{"key":"e_1_3_4_2_2","first-page":"238","volume-title":"Tropical Conservation: Perspectives on Local and Global Priorities","author":"Taber A","year":"2016","unstructured":"Taber A, , Beck H, , Gonzalez S, et al. Why neotropical forest ungulates matter, the case for deer, peccary and tapir conservation. In: Aguirre AA, , Sukumar R (eds). Tropical Conservation: Perspectives on Local and Global Priorities. New York: Oxford University Press; 2016: 238\u2013254."},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1017\/CBO9780511817359"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/9781444355833.ch11"},{"key":"e_1_3_4_5_2","first-page":"151","volume-title":"Mazama americana","author":"Varela DM","unstructured":"Varela DM, , Trovat IRG, , Guzman KR, et al. Red brocket deer Mazama americana (Erxleben 1777). In: Duarte JMB, Gonz\u00e1lez S (eds). Neotropical Cervidology. Jaboticabal, SP, Brazil: IUCN\/FUNEP; 2010: 151\u2013159."},{"key":"e_1_3_4_6_2","first-page":"541","article-title":"Collection, conservation and multiplication of genetic resources in wild animals: The example of deer","volume":"9","author":"Duarte JMB","year":"2005","unstructured":"Duarte JMB. 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