{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T20:13:51Z","timestamp":1787170431218,"version":"build-2736575974"},"reference-count":41,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Biopreservation and Biobanking"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>This study was designed to assess the effects of using tris-soybean lecithin (TSL)-based extender supplemented with bovine serum albumin (BSA) on the quality of ram epididymal spermatozoa during refrigerated storage.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method:<\/jats:title>\n                    <jats:p>Epididymal sperm were collected from 22 Zandi rams, diluted in TSL-based extender at different concentrations (0%, 2.5%, 5%, 7.5%, and 10%) of BSA, and stored for 5 days at 4\u00b0C. Sperm parameters including motility, viability, plasma membrane integrity, chromatin protamination, and malondialdehyde (MDA) content were evaluated at 0, 24, 72, and 120 hours of refrigeration.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      The addition of 10% BSA to the extender significantly improved sperm viability at 24 and 120 hours of refrigerated liquid storage (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      \u2009&lt;\u20090.05). An enhancement in plasma membrane integrity was observed along with a decrease in MDA level by increasing the concentration of BSA from 0% to 10% (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      \u2009&gt;\u20090.05). Sperm motion characteristics were higher in the BSA-free group at 120 hours of preservation (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      \u2009&lt;\u20090.05). No statistical difference was found for nuclear protamination between experimental groups (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      \u2009&gt;\u20090.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>BSA supplementation in TSL-based extender can preserve the viability of epididymal ram spermatozoa during liquid storage at 4\u00b0C.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/bio.2020.0041","type":"journal-article","created":{"date-parts":[[2020,11,4]],"date-time":"2020-11-04T15:43:43Z","timestamp":1604504623000},"page":"33-40","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Effect of Bovine Serum Albumin Supplementation in Tris-Soybean Lecithin-Based Extender on Quality of Chilled Ram Epididymal Spermatozoa"],"prefix":"10.1177","volume":"19","author":[{"given":"Pegah","family":"Rahimizadeh","sequence":"first","affiliation":[{"name":"Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tohid","family":"Rezaei Topraggaleh","sequence":"additional","affiliation":[{"name":"Department of Reproductive Biology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran."},{"name":"Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustafa Numan","family":"Bucak","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Selcuk University, Konya, Turkey."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niloofar","family":"Ziarati","sequence":"additional","affiliation":[{"name":"Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arash","family":"Hasirbaf","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyed Mohamad Javad","family":"Taher-Mofrad","sequence":"additional","affiliation":[{"name":"Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saman","family":"Maroufizadeh","sequence":"additional","affiliation":[{"name":"School of Nursing and Midwifery, Guilan University of Medical Sciences, Rasht, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdolhossein","family":"Shahverdi","sequence":"additional","affiliation":[{"name":"Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran."},{"name":"Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,2,9]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2011.11.003"},{"key":"e_1_3_6_3_2","first-page":"121","volume-title":"Cryopreservation in Eukaryotes","author":"Bertol MAF.","year":"2016","unstructured":"Bertol MAF. 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