{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T13:04:18Z","timestamp":1777467858851,"version":"3.51.4"},"reference-count":38,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Biopreservation and Biobanking"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:p>\n                    Vitamin E is one of the most powerful antioxidants for prevention of cell damage resulting from cryopreservation, but its efficacy for cryopreserving brown trout sperm is still unclear. In this work, the protective effect of vitamin E on quality, fertilizing capacity, and DNA damage of brown trout (\n                    <jats:italic toggle=\"yes\">Salmo trutta macrostigma<\/jats:italic>\n                    ) sperm after cryopreservation was evaluated. Sperm samples were diluted at the ratio of 1:10 with three different extenders (E): (E-I): 300\u2009mM glucose, 10% egg yolk; (E-II): 33.3\u2009mM glucose, 5.1\u2009mM NaCl, 0.5\u2009mM NaHCO\n                    <jats:sub>3,<\/jats:sub>\n                    , 15% DMA; and (E-III): 61.6\u2009mM NaCl, 134.2\u2009mM KCl, 1.9\u2009mM CaCl\n                    <jats:sub>2<\/jats:sub>\n                    , 0.8\u2009mM MgCl\n                    <jats:sub>2<\/jats:sub>\n                    , 2.3\u2009mM NaHCO\n                    <jats:sub>3<\/jats:sub>\n                    in distilled water. Each extender was supplemented with 10% DMSO and different concentrations of vitamin E at 0.1, 0.5, and 1.0\u2009mM. Spermatozoa frozen without vitamin E (0\u2009mM, control) and fresh sperm were also used. After dilution, the sperm was aspirated into 0.25\u2009mL straws, frozen 3\u2009cm above the liquid nitrogen (LN\n                    <jats:sub>2<\/jats:sub>\n                    ) surface, and plunged into the LN\n                    <jats:sub>2<\/jats:sub>\n                    . Cell motility, viability, fertilization, and eyeing were determined in post-thawed samples. DNA damage was determined by the comet assay after cryopreservation. Supplementation of 1\u2009mM vitamin E to all extenders exhibited the best cryoprotective effect in terms of sperm motility, duration of motility, viability, fertility, and DNA integrity against cryopreservation damage, compared with 0.1, 0.5, and control group (0\u2009mM) (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009&lt;\u20090.05). The highest post-thaw motility (62.4%\u2009\u00b1\u20090.36%), fertilization (48.2\u2009\u00b1\u20090.84), and the lowest DNA damage (7.245%) were obtained with the extender-II including 1.0\u2009mM vitamin E (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Consequently, vitamin E positively affected the motility parameters, fertility, and DNA integrity, and the results suggest the addition of extenders with vitamin E as an antioxidant for the cryopreservation of brown trout sperm.\n                  <\/jats:p>","DOI":"10.1089\/bio.2020.0062","type":"journal-article","created":{"date-parts":[[2020,11,17]],"date-time":"2020-11-17T17:21:59Z","timestamp":1605633719000},"page":"171-177","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":13,"title":["Cryoprotective Effect of Vitamin E Supplementation of Different Extenders on Quality and Fertilizing Ability of Frozen-Thawed Brown Trout Sperm"],"prefix":"10.1177","volume":"19","author":[{"given":"Yusuf","family":"Bozkurt","sequence":"first","affiliation":[{"name":"Department of Aquaculture, Faculty of Marine Sciences and Technology, \u0130skenderun Technical University, \u0130skenderun, Turkey."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u0130lker","family":"Yava\u015f","sequence":"additional","affiliation":[{"name":"Deparment of Reproduction and Insemination, Faculty of Veterinary Science, Mustafa Kemal University, Antakya, Turkey."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mustafa Numan","family":"Bucak","sequence":"additional","affiliation":[{"name":"Deparment of Reproduction and Artificial Insemination, Faculty of Veterinary Science, Sel\u00e7uk University, Konya, Turkey."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tu\u011fba Raika","family":"K\u0131ran","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Faculty of Engineering and Natural Sciences, \u0130skenderun Technical University, \u0130skenderun, Turkey."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aziz","family":"G\u00fcl","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Faculty of Agriculture, Mustafa Kemal University, Antakya, Turkey."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2021,6,9]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-45006-4"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2014.05.037"},{"key":"e_1_3_4_4_2","first-page":"293","volume-title":"Current Frontiers in Cryopreservation","author":"Bozkurt Y","year":"2012","unstructured":"Bozkurt Y, , Yava\u015f \u0130, , Karaca F. Cryopreservation of brown trout (Salmo trutta macrostigma) and ornamental koi carp (Cyprinus carpio) sperm, In: Katkov I. (ed). Current Frontiers in Cryopreservation. Rijeka, Croatia: InTech; 2012:293\u2013304."},{"key":"e_1_3_4_5_2","first-page":"275","article-title":"Effect of extender supplemented with boron on post-thaw motility, viability, DNA damage and fertilization ability of cryopreserved brown trout (Salmo trutta macrostigma) spermatozoa","volume":"40","author":"Bozkurt Y","year":"2019","unstructured":"Bozkurt Y, , Yava\u015f \u0130, G\u00fcl A, , Bucak MN, , Yeni D, , Avdatek F. Effect of extender supplemented with boron on post-thaw motility, viability, DNA damage and fertilization ability of cryopreserved brown trout (Salmo trutta macrostigma) spermatozoa. 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