{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T02:07:26Z","timestamp":1790129246957,"version":"4.0.1"},"reference-count":46,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Biopreservation and Biobanking"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:p>\n                    This study assessed the feasibility of\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    embryo production and nonsurgical embryo recovery (NSER) in Morada Nova ewes (an endangered native Brazilian breed of sheep) subjected to different estrus synchronization and\/or superovulation protocols. Ewes received intravaginal sponges soaked with 60\u2009mg medroxyprogesterone acetate (MAP), which were kept in place for six (G6;\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    \u2009=\u200912), nine (G9;\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    \u2009=\u200912), or 12 (G12;\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    \u2009=\u200912) days. Half of the ewes in each group remained estrus synchronized only (\n                    <jats:sub>SYNCH<\/jats:sub>\n                    ) and the other half was superovulated (\n                    <jats:sub>SOV<\/jats:sub>\n                    ) with 133\u2009mg porcine follicle-stimulating hormone (pFSH). There were no differences (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009&gt;\u20090.05) in antral follicle counts determined with ultrasonography 60 hours before MAP sponge removal (or at the time of the first pFSH dose) among G6 (6.4\u2009\u00b1\u20090.9), G9 (6.2\u2009\u00b1\u20090.7), and G12 (5.5\u2009\u00b1\u20090.6). Estrus responses and NSER success rates did not vary (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009&gt;\u20090.05) among the three progestin-treatment groups of ewes for either estrus-induced or superovulated animals. The onset of estrus occurred 10\u201312 hours later (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009&lt;\u20090.01) in G9\n                    <jats:sub>SYNCH<\/jats:sub>\n                    ewes compared with G6\n                    <jats:sub>SYNCH<\/jats:sub>\n                    and G12\n                    <jats:sub>SYNCH,<\/jats:sub>\n                    and the duration of estrus was \u223c19 hours greater (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009&lt;\u20090.01) in G9\n                    <jats:sub>SOV<\/jats:sub>\n                    than in G6\n                    <jats:sub>SOV<\/jats:sub>\n                    . The average duration of the NSER procedure was 32.6\u2009\u00b1\u20091.3 minutes. At least one structure was recovered in 85.7% of synchronized and in 87.5% of superovulated ewes. Viable embryo recovery rates were also similar (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009&gt;\u20090.05) for G6 (1.0\u2009\u00b1\u20090.3 and 2.5\u2009\u00b1\u20091.5), G9 (1.3\u2009\u00b1\u20090.5 and 4.8\u2009\u00b1\u20092.0), and G12 groups (1.0\u2009\u00b1\u20090.3 and 4.8\u2009\u00b1\u20092.3; estrus-synchronized and superovulated ewes, respectively). In conclusion, progestogen pretreatment of different durations and NSER can be employed in Morada Nova ewes, resulting in reasonable viable embryo recovery rates in both estrus-synchronized and superovulated animals. Therefore, both techniques are suitable for use in commercial settings as well as small ruminant conservation programs.\n                  <\/jats:p>","DOI":"10.1089\/bio.2020.0125","type":"journal-article","created":{"date-parts":[[2021,3,26]],"date-time":"2021-03-26T10:34:45Z","timestamp":1616754885000},"page":"360-368","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":16,"title":["NonSurgical Embryo Recovery from Estrus-Synchronized or Superovulated Morada Nova Ewes: A Feasible Strategy for Sheep Embryo Banking"],"prefix":"10.1177","volume":"19","author":[{"given":"Aline Matos","family":"Arrais","sequence":"first","affiliation":[{"name":"Departamento de Reprodu\u00e7\u00e3o e Avalia\u00e7\u00e3o Animal, Universidade Federal Rural do Rio de Janeiro, Serop\u00e9dica, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco Roberto Bourg de","family":"Mello","sequence":"additional","affiliation":[{"name":"Departamento de Reprodu\u00e7\u00e3o e Avalia\u00e7\u00e3o Animal, Universidade Federal Rural do Rio de Janeiro, Serop\u00e9dica, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel Brun","family":"Vergani","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias, Universidade Estadual Paulista, Jaboticabal, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas Machado","family":"Figueira","sequence":"additional","affiliation":[{"name":"Faculdade de Veterin\u00e1ria, Universidade Federal Fluminense, Niter\u00f3i, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9rgio Novita","family":"Esteves","sequence":"additional","affiliation":[{"name":"Embrapa Pecu\u00e1ria Sudeste, S\u00e3o Carlos, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ver\u00f4nica Schinaider do Amaral","family":"Pereira","sequence":"additional","affiliation":[{"name":"Embrapa Pecu\u00e1ria Sudeste, S\u00e3o Carlos, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pawel Mieczyslaw","family":"Bartlewski","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Emilia Franco","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias, Universidade Estadual Paulista, Jaboticabal, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna Maria Gon\u00e7alves","family":"Souza-Fabjan","sequence":"additional","affiliation":[{"name":"Faculdade de Veterin\u00e1ria, Universidade Federal Fluminense, Niter\u00f3i, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeferson Ferreira da","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Embrapa Caprinos e Ovinos, Coronel Pacheco, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,10,18]]},"reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(01)00668-9"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2011.01.009"},{"key":"e_1_3_5_4_2","unstructured":"Fac\u00f3 O Paiva SR Alves LRN et al. 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