{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T13:08:49Z","timestamp":1777468129905,"version":"3.51.4"},"reference-count":42,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Biopreservation and Biobanking"],"published-print":{"date-parts":[[2023,8,1]]},"abstract":"<jats:p>\n                    This study aimed to determine how melatonin (MT) and seminal plasma affected the freezability of buck sperm during the nonbreeding season. Semen was collected from eight bucks before (pre-MT) and after (post-MT) MT application in the nonbreeding season. Individual ejaculates were collected from the bucks, split into two equal groups according to the removal of seminal plasma (SP) (\u2212) or nonremoval of SP (+). For washing, the groups of ejaculates were centrifuged, and the supernatant was separated, SP (\u2212) and SP (+) ejaculates were diluted, then frozen. Semen samples were examined for sperm motility, plasma membrane integrity, defective acrosomes, DNA fragmentation, and mitochondrial membrane function at the native and post-thaw stages. When the general average post-thaw motility (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009&lt;\u20090.01), plasma membrane (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009&lt;\u20090.05), acrosome (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009&lt;\u20090.05), and DNA integrity rates (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009&lt;\u20090.05) and mitochondrial membrane potential (MMP) (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009&lt;\u20090.01) were evaluated, it was seen that MT administration caused a statistically significant improvement. The dramatic effect of nonremoval of seminal plasma on motility and plasma membrane integrity is more clearly observed in individual semen samples frozen in the pre-MT group (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Also, it was observed that removing seminal plasma in the post-MT group caused even milder post-thaw acrosome damage compared with the SP (+) group (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009&lt;\u20090.05). The effect of removing seminal plasma was not observed in terms of DNA integrity and MMP rates in pre- and post-MT groups. As a result, it was concluded that MT application and removal of seminal plasma in the nonbreeding season result in improvement in the freezability of buck semen.\n                  <\/jats:p>","DOI":"10.1089\/bio.2022.0074","type":"journal-article","created":{"date-parts":[[2022,9,20]],"date-time":"2022-09-20T17:15:56Z","timestamp":1663694156000},"page":"327-335","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["The Combined Effect of Melatonin Implant and Removal of Buck Seminal Plasma on Cryopreservation During the Nonbreeding Season"],"prefix":"10.1177","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5999-4685","authenticated-orcid":false,"given":"Burcu","family":"Ustuner","sequence":"first","affiliation":[{"name":"Department of Reproduction and Artificial Insemination and Faculty of Veterinary Medicine, Uludag University, Bursa, Turkey."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hakan","family":"Ustuner","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Faculty of Veterinary Medicine, Uludag University, Bursa, Turkey."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elif","family":"Gokce","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Nam\u0131k Kemal University, Tekirdag, Turkey."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. Tekin","family":"Onder","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Kafkas University, Kars, Turkey."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. Melih","family":"Yilmaz","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination and Faculty of Veterinary Medicine, Uludag University, Bursa, Turkey."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4125-6841","authenticated-orcid":false,"given":"Oguzhan","family":"Huraydin","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination and Faculty of Veterinary Medicine, Uludag University, Bursa, Turkey."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. Berk","family":"Toker","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination and Faculty of Veterinary Medicine, Uludag University, Bursa, Turkey."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2023,8,17]]},"reference":[{"issue":"446","key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.cca.2015.04.029","article-title":"Melatonin and male reproduction","author":"Li C","year":"2015","unstructured":"Li C, , Zhou X. Melatonin and male reproduction. 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