{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T05:25:33Z","timestamp":1785216333921,"version":"3.55.0"},"reference-count":32,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Biopreservation and Biobanking"],"published-print":{"date-parts":[[2025,6,1]]},"abstract":"<jats:p>\n                    The peroxidation of spermatozoa membrane phospholipids is a primary cause of irreversible changes in the preservation of avian semen. To address this issue, the objective of the present study was to assess the potential of\n                    <jats:italic toggle=\"yes\">Urtica dioica<\/jats:italic>\n                    extracts in protecting avian spermatozoa during prolonged storage. Gas chromatography-mass spectroscopic techniques were employed to evaluate the bioactive compounds present in the aqueous and ethanolic extracts obtained from the aerial parts and roots of\n                    <jats:italic toggle=\"yes\">U. dioica<\/jats:italic>\n                    . Semen samples were collected from 16 roosters twice a week and were diluted in Lake\u2019s extender containing different concentrations (0, 0.5, and 1 mg\/100 mL) of the various extracts. Subsequently, the extended semen samples were cooled and stored at 5\u00b0C, and the sperm quality parameters were assessed at 0, 12, 24, and 36 hours of storage. The data from this experiment clearly demonstrate that the addition of nettle root aqueous extracts to the semen diluent, especially at a concentration of 0.5 mg\/100 mL, resulted in a significant improvement in various sperm quality parameters. Notably, there were enhancements in total and progressive sperm motilities, viability, fertility, membrane integrity, acrosomal membrane integrity, and a reduction in malondialdehyde production in rooster semen stored\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    for up to 36 hours. Interestingly, the present study reveals that the beneficial effects of the aqueous extracts from different parts of the nettle were supported not only by the conventional manual method but also by the computer-assisted sperm analysis system. This dual confirmation further emphasizes the positive impact of the aqueous extract on various sperm traits during cooled semen preservation. In conclusion, this study highlights the potential of\n                    <jats:italic toggle=\"yes\">U. dioica<\/jats:italic>\n                    extracts, particularly the aqueous extract from nettle roots at a concentration of 0.5 mg\/100 mL, in safeguarding avian spermatozoa during prolonged storage. The significant improvements in various sperm quality parameters and the validation of results through both manual and computer-assisted analysis methods provide strong evidence for the application of\n                    <jats:italic toggle=\"yes\">U. dioica<\/jats:italic>\n                    extracts in avian breeding programs and artificial insemination practices.\n                  <\/jats:p>","DOI":"10.1089\/bio.2022.0165","type":"journal-article","created":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T12:50:17Z","timestamp":1725972617000},"page":"225-235","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Supplementation of Rooster Semen Extender with Aqueous Extract of\n                    <i>Urtica dioica<\/i>\n                    \u00a0for a Long Time Preservation by Low Temperature"],"prefix":"10.1177","volume":"23","author":[{"given":"Sina","family":"Naderi","sequence":"first","affiliation":[{"name":"Department of Animal Science, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1172-9385","authenticated-orcid":false,"given":"Amjad","family":"Farzinpour","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asaad","family":"Vaziry","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1502-7797","authenticated-orcid":false,"given":"Abbas","family":"Farshad","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,6,12]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.3382\/ps.0170408"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.3382\/ps.0621305"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1080\/00071667808416462"},{"key":"e_1_3_4_5_2","first-page":"615","article-title":"Uterine blood flow evaluation after artificial insemination on different uterine sites in mares","volume":"10","author":"Araujo AG","year":"2013","unstructured":"Araujo AG, , Brandao FZ, , Guedes MC, et al. Uterine blood flow evaluation after artificial insemination on different uterine sites in mares. Anim. Reprod, 2013; 10:615.","journal-title":"Anim. Reprod"},{"key":"e_1_3_4_6_2","first-page":"13","article-title":"The effect of different concentration of fallopian tubes (oviducts) secretion extract on turkey spermatozoa motility in vitro","volume":"2","author":"Mi\u0161keje M","year":"2013","unstructured":"Mi\u0161keje M, , Slanina T, , Petrovi\u010dov\u00e1 I, , Mass\u00e1nyi P. The effect of different concentration of fallopian tubes (oviducts) secretion extract on turkey spermatozoa motility in vitro. 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Evaluation of in-vitro antioxidant properties of hydroalcoholic solution extracts Urtica dioica L., Malva neglecta Wallr. and their mixture. Iran J Pharm Res, 2012; 11(3):913\u2013923.","journal-title":"Iran J Pharm Res"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aninu.2019.04.004"},{"key":"e_1_3_4_15_2","first-page":"5","volume-title":"Index to the Scientific Names of the 2000 Plants Presented Flora of Iran in Natural Colors","author":"Ghahreman A","year":"1996","unstructured":"Ghahreman A. Index to the Scientific Names of the 2000 Plants Presented Flora of Iran in Natural Colors. Research Institute of Forestes and Rangelands: Tehran; 1996; pp. 5."},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2016.10.009"},{"key":"e_1_3_4_17_2","volume-title":"Techniques for Semen Evaluation, Semen Storage, and Fertility Determination","author":"Bakst MR","year":"1997","unstructured":"Bakst MR, , Cecil HC. 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Protective effect of Urtica dioica L against nicotine-induced damage on sperm parameters, testosterone and testis tissue in mice. Iran J Reprod Med, 2014; 12(6):401\u2013408.","journal-title":"Iran J Reprod Med"},{"key":"e_1_3_4_32_2","first-page":"652","article-title":"The original Croatian pharmacopoeia from 1901","volume":"67","author":"Ini\u0107 S","year":"2012","unstructured":"Ini\u0107 S, , Kujund\u017ei\u0107 N. The original Croatian pharmacopoeia from 1901. Die Pharmazie-An Int J Pharm Sci, 2012; 67:652\u2013657.","journal-title":"Die Pharmazie-An Int J Pharm Sci"},{"key":"e_1_3_4_33_2","first-page":"461","article-title":"Some post-cryopreserved semen characteristics of Holstein Bulls as influenced by adding aquoeus extract of Urtica dioica and date palm pollen powder to Tris extender","volume":"1","author":"Mohamed OA","year":"2020","unstructured":"Mohamed OA, , Abdulkareem TA. Some post-cryopreserved semen characteristics of Holstein Bulls as influenced by adding aquoeus extract of Urtica dioica and date palm pollen powder to Tris extender. 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