{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T13:10:31Z","timestamp":1777468231342,"version":"3.51.4"},"reference-count":46,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Biopreservation and Biobanking"],"published-print":{"date-parts":[[2024,10,1]]},"abstract":"<jats:p>\n                    The ability to cryopreserve oocytes without ultrastructural injury has been a concern in the development and use of methods to preserve female reproduction. The stability of the cell membrane must be preserved to reduce the damage caused by ice crystals during vitrification. One approach that has been explored is the use of static magnetic fields (SMFs), which are believed to influence cell membrane stability. In this study, the\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    effects of SMF that range between 20\u201380 mT on the vitrification of mice germinal vesicle (GV) oocytes were studied. The viability and mitochondrial (Mt) membrane potential of both vitrified and nonvitrified oocytes were assessed using Trypan blue and JC1 staining. The high\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    maturation (IVM) rate and high Mt membrane potential in metaphase II (MII) oocytes were taken into account to determine the optimal magnetic field intensity, that is, 20 mT. None of the SMF conditions resulted in intact spindles in MII oocytes. The study also explored the expression of store-operated calcium entry (\n                    <jats:italic toggle=\"yes\">Stim1<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">Orai1<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">Ip3r<\/jats:italic>\n                    ) and meiosis resumption (\n                    <jats:italic toggle=\"yes\">Ccnb<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">Cdk<\/jats:italic>\n                    ) genes in GV and MII oocytes of both vitrified and control groups. The results show that the expressions of\n                    <jats:italic toggle=\"yes\">Orai1<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">Ccnb<\/jats:italic>\n                    genes in Vit-MII-SMF oocytes were considerably increased. However, no significant difference in\n                    <jats:italic toggle=\"yes\">Stim1<\/jats:italic>\n                    expression was observed between the groups. The Vit-MII-SMF group exhibited a significantly higher\n                    <jats:italic toggle=\"yes\">Ccnb<\/jats:italic>\n                    expression compared to other groups.\n                    <jats:italic toggle=\"yes\">In vitro<\/jats:italic>\n                    fertilization (IVF) was performed to evaluate the 2 pronuclear (2PN) rates. The findings demonstrated that using 20 mT SMF improved 2PN rates compared to the nonvitrified groups. This study provides a deeper understanding of the effects of moderate SMF and vitrification on the expression of calcium channel genes in GV and MII oocytes. The results suggest that applying a 20 mT SMF can help prevent cryoinjury and enhance the characteristics of vitrified-warmed oocytes.\n                  <\/jats:p>","DOI":"10.1089\/bio.2022.0200","type":"journal-article","created":{"date-parts":[[2024,3,25]],"date-time":"2024-03-25T16:54:37Z","timestamp":1711385677000},"page":"441-451","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Effect of Moderate Static Magnetic Fields on Mice Oocyte Vitrification: Calcium-Related Genes Expression"],"prefix":"10.1177","volume":"22","author":[{"given":"Sara Soleimani","family":"Pargoo","sequence":"first","affiliation":[{"name":"Department of Cell and Molecular Biology, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran."},{"name":"Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farzaneh","family":"Baniasadi","sequence":"additional","affiliation":[{"name":"Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vida Sadat Kazemein","family":"Jasemi","sequence":"additional","affiliation":[{"name":"Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samira","family":"Hajiaghalou","sequence":"additional","affiliation":[{"name":"Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohsen","family":"Gharanfoli","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3871-5207","authenticated-orcid":false,"given":"Rouhollah","family":"Fathi","sequence":"additional","affiliation":[{"name":"Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2024,10,21]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.09.014"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-6483(10)60837-1"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1530\/rep.1.00878"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2019.09.012"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/asj.12061"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)49895-5"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e13-01-0065"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1995.1240"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2017.02.004"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2005.02.035"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1006\/scdb.2000.0211"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90430-1"},{"issue":"24","key":"e_1_3_3_14_2","first-page":"5714","article-title":"Regulation of endoplasmic reticulum Ca2+ oscillations in mammalian eggs","volume":"126","author":"Wakai T","year":"2013","unstructured":"Wakai T, , Zhang N, , Vangheluwe P, et al. Regulation of endoplasmic reticulum Ca2+ oscillations in mammalian eggs. J Cell Sci, 2013; 126(24):5714\u20135724.","journal-title":"J Cell Sci"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1112333109"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.3109\/15368378.2014.919588"},{"issue":"3","key":"e_1_3_3_17_2","first-page":"243","article-title":"The function of vitrified mouse ovaries tissue under static magnetic field after autotransplantation","volume":"8","author":"Jasemi VSK","year":"2017","unstructured":"Jasemi VSK, , Samadi F, , Eimani H, et al. The function of vitrified mouse ovaries tissue under static magnetic field after autotransplantation. 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