{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T20:30:49Z","timestamp":1791923449551,"version":"4.3.4"},"reference-count":45,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Biopreservation and Biobanking"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:p>\n                    This study aimed to assess the suitability of egg yolk (EY) supplementation to a tris\u2013citric acid\u2013based extender on cryosurvival of guinea pig (\n                    <jats:italic toggle=\"yes\">Cavia porcellus<\/jats:italic>\n                    ) epididymal spermatozoa. Two synthetic-based extenders, tris\u2013citric acid\u2013glucose plus 20% EY (TCG-EY) and tris\u2013citric acid\u2013fructose (TCF) both with 5% glycerol, were compared. Thirty-two epididymides were recovered from 16 adult guinea pig males by gonadectomy, and then the sperm samples were retrieved by retrograde flushing using TCG-EY and TCF extenders for left or right epididymis, respectively. TCG-EY and TCF sperm samples were frozen in static liquid nitrogen vapors through a two-step cooling procedure. Before freezing, the percentage of progressive sperm motility and sperm with intact plasma and acrosome membranes from TCG-EY sperm samples were higher (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009&lt;\u20090.05) than those diluted with TCF. Post-thaw sperm kinematic variables and membrane integrity were drastically reduced (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009&lt;\u20090.001) compared with prefreezing samples, regardless of extender type. The post-thaw plasmatic and acrosome membrane integrity from TCG-EY sperm samples was higher (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009&lt;\u20090.05) than those from TCF samples. Except for the length, the morphometric head dimensions of sperm diluted with TCG-EY or TCF did not vary (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009&gt;\u20090.05) after the freezing\u2013thawing process compared with the prefreezing samples. In conclusion, despite greater cell cryoinjury with both extenders, the EY supplementation exerted greater cell membrane protection before and after the freezing\u2013thawing process. This research shows an in-depth analysis of guinea pig sperm cryopreservation; however, more studies are recommended.\n                  <\/jats:p>","DOI":"10.1089\/bio.2022.0214","type":"journal-article","created":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T14:46:57Z","timestamp":1692283617000},"page":"157-165","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Egg Yolk-Supplemented Tris\u2013Citric Acid Extender Improves the Prefreezing and Post-Thaw Sperm Quality Indices of Guinea Pig (\n                    <i>Cavia porcellus<\/i>\n                    ) Epididymal Spermatozoa"],"prefix":"10.1177","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0266-5431","authenticated-orcid":false,"given":"Diego A.","family":"Galarza","sequence":"first","affiliation":[{"name":"Laboratorio de Biotecnolog\u00eda de la Reproducci\u00f3n Animal, Facultad de Ciencias Agropecuarias, Universidad de Cuenca, Cuenca, Ecuador."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilson","family":"Le\u00f3n-Machuca","sequence":"additional","affiliation":[{"name":"Laboratorio de Sanidad Animal y Zoonosis, Departamento de Ciencias Biol\u00f3gicas y Agropecuarias, Universidad T\u00e9cnica Particular de Loja, Loja, Ecuador."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge X.","family":"Samaniego","sequence":"additional","affiliation":[{"name":"Laboratorio de Biotecnolog\u00eda de la Reproducci\u00f3n Animal, Facultad de Ciencias Agropecuarias, Universidad de Cuenca, Cuenca, Ecuador."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rub\u00e9n","family":"Carrera","sequence":"additional","affiliation":[{"name":"Laboratorio de Sanidad Animal y Zoonosis, Departamento de Ciencias Biol\u00f3gicas y Agropecuarias, Universidad T\u00e9cnica Particular de Loja, Loja, Ecuador."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando P.","family":"Perea","sequence":"additional","affiliation":[{"name":"Laboratorio de Biotecnolog\u00eda de la Reproducci\u00f3n Animal, Facultad de Ciencias Agropecuarias, Universidad de Cuenca, Cuenca, Ecuador."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4684-8034","authenticated-orcid":false,"given":"Mauricio","family":"Duma","sequence":"additional","affiliation":[{"name":"Laboratorio de Biotecnolog\u00eda de la Reproducci\u00f3n Animal, Facultad de Ciencias Agropecuarias, Universidad de Cuenca, Cuenca, Ecuador."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juli\u00e1n","family":"Santiago-Moreno","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction, INIA-CSIC, Madrid, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,4,15]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meatsci.2019.02.012"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1017\/S2078633614000368"},{"key":"e_1_3_4_4_2","unstructured":"Maass BL Chauca-Francia L Chiuri WL et al. From \u2018cuy\u2019 in South America to \u2018cavy\u2019 in Sub-Sahara Africa: Advancing development through South-South Cooperation. Advancing development through South-South Cooperation. In: Paper presented at the Tropentag 2016 Conference on Solidarity in a Competing World\u2014Fair Use of Resources Vienna Austria 19\u201321 September 2016. University of G\u00f6ttingen: G\u00f6ttingen Germany."},{"key":"e_1_3_4_5_2","unstructured":"HLPE. Sustainable agricultural development for food security and nutrition: what roles for livestock? A report by the High Level Panel of Experts on Food Security and Nutrition of the Committee on World Food Security Rome. 2016. 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