{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T13:10:20Z","timestamp":1777468220353,"version":"3.51.4"},"reference-count":46,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T00:00:00Z","timestamp":1722470400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Biopreservation and Biobanking"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:p>\n                    We researched the ability of tanacetan pectin from inflorescences of common tansy\n                    <jats:italic toggle=\"yes\">Tanacetum vulgare<\/jats:italic>\n                    L. to change the osmolarity and freezing point of water in solutions of cryoprotectants: glycerol\u20143.5%, dimethyl sulfoxide (DMSO)\u201410%, dimethylacetamide\u201410% (DMAC), and 1.2-propanediol (1.2-PD)\u201410%, as well as the effect of solutions of tanacetan (0.2%, 0.4%) on the kinetics of crystallization processes and the nature of crystal formation. We used a combination of protector and pectin that we tested earlier, which provided effective protection for human leukocytes and platelets, as well as bovine spermatozoa, at temperatures below freezing (\u221220\u00b0C and \u221280\u00b0C). It has been established that tanacetan slows down the process of water freezing in glycerol, but not in DMSO, DMAC, and 1.2-PD, promotes deeper supercooling of the medium, and affects the morphological structure of ice. The addition of pectin to the cryosolution increases the activity of the main cryoprotectant glycerol even at its low concentrations. The combination of glycerol and tanacetan can be effective in freezing biological materials, which is confirmed by the preservation of leukocytes at \u221220\u00b0C and \u221280\u00b0C for 7 days, platelets at \u221280\u00b0C for 30 days, and spermatozoa at \u221280\u00b0C within 1 day. A comprehensive analysis of the chemical, physicochemical, and cryoprotective properties of tanacetan indicates the prospect of using pectin in the cryopreservation of biological objects at temperatures of electric freezers.\n                  <\/jats:p>","DOI":"10.1089\/bio.2023.0066","type":"journal-article","created":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T11:21:27Z","timestamp":1704712887000},"page":"336-345","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Cryoprotective Effect of Pectin Tanacetan from\n                    <i>Tanacetum vulgare<\/i>\n                    L"],"prefix":"10.1177","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4999-3077","authenticated-orcid":false,"given":"Tatyana Vitalyevna","family":"Polezhaeva","sequence":"first","affiliation":[{"name":"Institute of Physiology of Komi Scientific Centre of the Ural Branch of the Russian Academy of Sciences, FRC Komi SC UB RAS, Syktyvkar, Russia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oksana Olegovna","family":"Zaitseva","sequence":"additional","affiliation":[{"name":"Institute of Physiology of Komi Scientific Centre of the Ural Branch of the Russian Academy of Sciences, FRC Komi SC UB RAS, Syktyvkar, Russia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrey Nikolayevich","family":"Khudyakov","sequence":"additional","affiliation":[{"name":"Institute of Physiology of Komi Scientific Centre of the Ural Branch of the Russian Academy of Sciences, FRC Komi SC UB RAS, Syktyvkar, Russia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3113-527X","authenticated-orcid":false,"given":"Marta Igorevna","family":"Sergushkina","sequence":"additional","affiliation":[{"name":"Institute of Physiology of Komi Scientific Centre of the Ural Branch of the Russian Academy of Sciences, FRC Komi SC UB RAS, Syktyvkar, Russia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga Nurzadinovna","family":"Solomina","sequence":"additional","affiliation":[{"name":"Institute of Physiology of Komi Scientific Centre of the Ural Branch of the Russian Academy of Sciences, FRC Komi SC UB RAS, Syktyvkar, Russia."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2024,8,19]]},"reference":[{"key":"e_1_3_5_2_2","unstructured":"Safonov NN. 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