{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T10:31:09Z","timestamp":1782988269811,"version":"3.54.5"},"reference-count":42,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Biopreservation and Biobanking"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:p>\n                    Cryopreservation of avian semen is a useful reproductive technique in the poultry industry. However, during cooling, elevated reactive oxygen species (ROS) levels have destructive effects on both quality and function of thawed sperm. The aim of the current study is to investigate the antioxidant effects of\n                    <jats:italic toggle=\"yes\">N<\/jats:italic>\n                    -acetylcysteine (NAC) during rooster semen cryopreservation. Semen samples were collected from ten Ross 308 broiler breeder roosters (32 weeks) and mixed. The mixed samples were divided into five equal parts and cryopreserved in Lake Buffer extender that contained different concentrations (0, 0.01, 0.1, 1, and 10 mM) of NAC. The optimum concentration of NAC was determined based on quality parameters of mobility, viability, membrane integrity, acrosome integrity, lipid peroxidation, and mitochondrial membrane potential after the freeze-thaw process. There was a higher percentage (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    &lt; 0.05) of total motility (TM) (60.9\u2009\u00b1\u20092.4%) and progressive motility (PM) (35.6\u2009\u00b1\u20091.9%) observed with the NAC-0.1 group compared to the other groups. Significantly higher percentages of viability (74.4 \u00b1 2.3% and 71 \u00b1 2.3%), membrane integrity (76.4 \u00b1 1.5% and 74.7 \u00b1 1.5%) and mitochondrial membrane potential (67.1 \u00b1 1.6% and 66.3 \u00b1 1.6%) were observed in the NAC-0.1 and NAC-1 groups compared to the other frozen groups (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    &lt; 0.05). The lowest percentage of lipid peroxidation and nonviable sperm was found in the NAC-0.1 and NAC-1 groups compared to the other groups (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    &lt; 0.05). The average path velocity (VAP), straight line velocity (VSL), curvilinear velocity (VCL), and acrosome integrity, were not affected by different concentrations of NAC in the thawed sperm (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    &gt; 0.05). Both NAC-0.1 and NAC-1 appear to be beneficial for maintaining the quality of rooster sperm after thawing.\n                  <\/jats:p>","DOI":"10.1089\/bio.2023.0103","type":"journal-article","created":{"date-parts":[[2024,4,18]],"date-time":"2024-04-18T08:47:15Z","timestamp":1713430035000},"page":"609-615","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Protective Effect of\n                    <i>N<\/i>\n                    -Acetylcysteine on Rooster Semen Cryopreservation"],"prefix":"10.1177","volume":"22","author":[{"given":"Farhad","family":"Hakimi","sequence":"first","affiliation":[{"name":"Department of Animal Sciences, College of Agriculture, Tarbiat Modares University, Tehran, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamad Amir","family":"Karimi Torshizi","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, College of Agriculture, Tarbiat Modares University, Tehran, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1460-1862","authenticated-orcid":false,"given":"Maryam","family":"Hezavehei","sequence":"additional","affiliation":[{"name":"Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran."},{"name":"Research Center for Reproduction and Fertility, Faculty of Veterinary medicine, Montreal University, St-Hyacinthe, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohsen","family":"Sharafi","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, College of Agriculture, Tarbiat Modares University, Tehran, Iran."},{"name":"Semex Alliance, Guelph, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,12,18]]},"reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.apjr.2016.01.009"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.psj.2021.101290"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2018.05.012"},{"key":"e_1_3_5_5_2","volume-title":"Artificial Insemination in Poultry","author":"Aisha K","year":"2010","unstructured":"Aisha K, , Zain UA. 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