{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T13:10:54Z","timestamp":1777468254536,"version":"3.51.4"},"reference-count":44,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Biopreservation and Biobanking"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:p>Many cellular processes in spermatozoa, including apoptosis and motility, are regulated by miRNA. Different miRNAs and molecular pathways are involved in asthenozoospermia (AS) conditions, which are thought to be one of the causes of infertility with reduced sperm motility. Thirty-two semen samples from four Holstein bulls with normozoospermia (NS), total motility \u2265 70%, and progressive motility \u2265 60%, and 32 semen samples from four bulls with AS, total motility \u2264 40%, and progressive motility \u2264 32% were used to investigate the function of apoptosis-related miRNAs in the AS group. Samples were then aspirated into a 0.5 mL straw after dilution with a Tris-egg yolk extender and frozen at \u2212196\u00b0C. After freezing, semen samples were thawed for 2 weeks at 37\u00b0C and sperm kinematic parameters, plasma membrane integrity, acrosome integrity, DNA fragmentation, apoptosis status, and expression of apoptosis-related miRNAs (miR-2114, miR-296-3p, miR-455-3p, and miR345-3p) were evaluated. Our results showed that the functional and flow cytometric parameters of the NS group were significantly better than those of the AS group. In the NS group, miR-455-3pp and miR-2412 were upregulated, while miR-345-3p was downregulated compared with the AS group. In the AS group, miR-296-39, miR-2412, and miR-345-3p levels were strongly correlated with membrane integrity, DNA fragmentation, and apoptosis status. The findings demonstrated that the selected miRNAs based on bioinformatic analysis in AS and NS samples had a substantial association with functional and flow cytometry indicators and may be involved in regulating apoptosis and motility in AS samples.<\/jats:p>","DOI":"10.1089\/bio.2023.0135","type":"journal-article","created":{"date-parts":[[2024,4,30]],"date-time":"2024-04-30T04:30:06Z","timestamp":1714451406000},"page":"616-627","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Apoptotic-Related MiRNAs Correlated with Functional and Flow Cytometric Parameters in Asthenozoospermic Holstein Bulls After Freeze\u2013Thaw Process"],"prefix":"10.1177","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9485-2073","authenticated-orcid":false,"given":"Morteza","family":"Taravat","sequence":"first","affiliation":[{"name":"Department of Clinical Science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amirmahdi","family":"Roshanzamir","sequence":"additional","affiliation":[{"name":"Department of Theriogenology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maryam","family":"Rahbar","sequence":"additional","affiliation":[{"name":"Department of Clinical Science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tohid Rezaei","family":"Topraggaleh","sequence":"additional","affiliation":[{"name":"Department of Anatomical Science, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reza","family":"Asadpour","sequence":"additional","affiliation":[{"name":"Department of Clinical Science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mustafa Numan","family":"Bucak","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Selcuk University, Konya, Turkey."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2024,12,18]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2019-17430"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17101712"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2013-6693"},{"key":"e_1_3_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2017.10.014"},{"key":"e_1_3_6_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dez114"},{"key":"e_1_3_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2018.12.007"},{"key":"e_1_3_6_8_2","doi-asserted-by":"publisher","DOI":"10.1080\/15384101.2020.1750814"},{"key":"e_1_3_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2010.06.013"},{"key":"e_1_3_6_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep17991"},{"key":"e_1_3_6_11_2","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2018.00402"},{"key":"e_1_3_6_12_2","doi-asserted-by":"publisher","DOI":"10.1261\/rna.1963810"},{"key":"e_1_3_6_13_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-018-5132-9"},{"issue":"5","key":"e_1_3_6_14_2","first-page":"2151","article-title":"miR-296-3p promotes the proliferation of glioblastoma cells by targeting ICAT","volume":"21","author":"Zhou J","year":"2020","unstructured":"Zhou J, , Du G, , Fu H. miR-296-3p promotes the proliferation of glioblastoma cells by targeting ICAT. 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