{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T15:11:47Z","timestamp":1788448307838,"version":"build-2803163510"},"reference-count":41,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Biopreservation and Biobanking"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:sec>\n                    <jats:title>Introduction:<\/jats:title>\n                    <jats:p>Sperm cryopreservation is a useful storage technique in artificial insemination. Nanoparticles and nanovesicles such as exosomes are widely used in sperm cryopreservation procedures to alleviate cold-induced injury inflicted during sperm freezing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>The objective of the present study was to examine the impact of varying concentrations of exosomes derived from seminal plasma added to a freezing extender on the quality of post-thawed bull sperm.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      Five Holstein bulls were chosen based on their samples having less than 30% progressive motility. After exosome extraction, semen samples from bulls (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 5) with progressive sperm motility \u226430% were collected, diluted with different exosome concentrations (0, 25, 50, and 100 \u03bcg\/mL), and aspirated into 0.5 mL straws. After the freeze\u2013thaw process, sperm total and progressive motility, viability, morphology, plasma membrane integrity, mitochondrial activity, and apoptosis status were assessed. Furthermore, the expression levels of annexin (ANX1), dystrophy-associated Fer-1-like protein (DYSF), fibronectin 1 (FN1), and reactive oxygen species modulator 1 (ROMO1) were evaluated via real-time polymerase chain reaction (PCR).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Adding different concentrations of exosomes (25, 50, and 150 \u03bcg\/mL) significantly increased the progressive motility, viability, and membrane integrity of sperm compared with the control group (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      &lt; 0.05). For the apoptosis index, treatment with 100 \u03bcg\/mL exosomes significantly increased the percentage of live cells (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      &lt; 0.05), while the percentage of necrotic cells decreased significantly (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      &lt; 0.05) compared with 25 \u03bcg\/mL exosome. The results of quantitative PCR showed that the expression levels of ANX1 were significantly (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      &lt; 0.05) upregulated at 50 \u03bcg\/mL exosome, and the expression of ROMO1, FN1, and DYSF were downregulated upon treatment with different exosome concentrations.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>In conclusion, supplementing the freezing diluent with exosome-derived seminal plasma could preserve the quality parameters of the post-thaw semen of the bull with low freezeability and could be used as a helpful method for reproductive programs.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/bio.2024.0077","type":"journal-article","created":{"date-parts":[[2024,12,26]],"date-time":"2024-12-26T04:08:42Z","timestamp":1735186122000},"page":"364-373","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":12,"title":["Seminal Plasma-Derived Exosome Preserves the Quality Parameters of the Post-Thaw Semen of Bulls with Low Freezeability"],"prefix":"10.1177","volume":"23","author":[{"given":"Rahele Ranjbar","family":"Shamsi","sequence":"first","affiliation":[{"name":"Department of Clinical Science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Razi Jafari","family":"Jozani","sequence":"additional","affiliation":[{"name":"Department of Clinical Science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reza","family":"Asadpour","sequence":"additional","affiliation":[{"name":"Department of Clinical Science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryam","family":"Rahbar","sequence":"additional","affiliation":[{"name":"Department of Clinical Science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9485-2073","authenticated-orcid":false,"given":"Morteza","family":"Taravat","sequence":"additional","affiliation":[{"name":"Reproductive Health Research Center, Clinical Research Institute, Urmia University of Medical Science, Urmia, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,8,19]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2019.00268"},{"key":"e_1_3_4_3_2","first-page":"55","volume-title":"Sustainable Agriculture Reviews","author":"Gherissi DE","year":"2021","unstructured":"Gherissi DE, , Lamraoui R. 2021. Reproduction management and artificial insemination in dromedary camel. Sustainable Agriculture Reviews, 54: Anim Biotechnol 2021; 55\u2013106; doi:10.1007\/978-3-030-76529-3_2"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2021.106904"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1089\/bio.2022.0097"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jevs.2023.104499"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-61803-8"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1080\/10495398.2019.1657126"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10010092"},{"issue":"12","key":"e_1_3_4_10_2","article-title":"Myoinositol supplementation of freezing medium improves the quality-related parameters of dog sperm","volume":"9","author":"Qamar AY","year":"2019","unstructured":"Qamar AY, , Fang X, , Kim MJ, et al. Myoinositol supplementation of freezing medium improves the quality-related parameters of dog sperm. Animals (Basel), 2019; 9(12); doi: 10.3390\/ani9121038","journal-title":"Animals (Basel)"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.5152\/tud.2019.30660"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2020.10.011"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.4068\/cmj.2019.55.3.136"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.3390\/ani10122281"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2020.608178"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2019.04.055"},{"key":"e_1_3_4_17_2","article-title":"Effects of hyaluronic acid on sperm parameters, mitochondrial function and apoptosis of spermatozoa in Simmental bulls with good and poor freezing ability","author":"Asadpour R","year":"2023","unstructured":"Asadpour R, , Aminirad M, , Rahbar M, et al. Effects of hyaluronic acid on sperm parameters, mitochondrial function and apoptosis of spermatozoa in Simmental bulls with good and poor freezing ability. J Anim Physio Anim Nutr, 2023; doi: 10.1111\/jpn.13900","journal-title":"J Anim Physio Anim Nutr"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1080\/19396368.2024.2306420"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2023.103992"},{"issue":"2","key":"e_1_3_4_20_2","first-page":"294","article-title":"Investigation of the effect of seminal plasma exosomes from the normal and oligoasthenoteratospermic males in the implantation process","volume":"12","author":"Gholipour H","year":"2023","unstructured":"Gholipour H, , Amjadi FS, , Zandieh Z, et al. Investigation of the effect of seminal plasma exosomes from the normal and oligoasthenoteratospermic males in the implantation process. Rep Biochem Mol Biol, 2023; 12(2):294\u2013305.","journal-title":"Rep Biochem Mol Biol"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1089\/bio.2023.0135"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2018.12.003"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2015.06.008"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.3390\/antiox9020134"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2022.112752"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/burnst\/tkaa007"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.11315"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1530\/REP-18-0639"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2021.09.031"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2022.03.033"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.2217\/nnm-2020-0056"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.livsci.2023.105298"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aau6977"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0303479"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/prot.26512"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21197022"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.9b00795"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.ade7607"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.4068\/cmj.2022.58.3.91"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad6780"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky817"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.3390\/ani9110865"}],"container-title":["Biopreservation and Biobanking"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/bio.2024.0077","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/bio.2024.0077","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/bio.2024.0077","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T13:33:30Z","timestamp":1777383210000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/bio.2024.0077"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8]]},"references-count":41,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,8]]}},"alternative-id":["10.1089\/bio.2024.0077"],"URL":"https:\/\/doi.org\/10.1089\/bio.2024.0077","relation":{},"ISSN":["1947-5535","1947-5543"],"issn-type":[{"value":"1947-5535","type":"print"},{"value":"1947-5543","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8]]}}}