{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T00:10:11Z","timestamp":1779927011467,"version":"3.53.1"},"reference-count":0,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2009,6,1]],"date-time":"2009-06-01T00:00:00Z","timestamp":1243814400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Biotherapy and Radiopharmaceuticals"],"published-print":{"date-parts":[[2009,6]]},"abstract":"<jats:p>\n                    We have studied CD4\n                    <jats:sup>+<\/jats:sup>\n                    CD25\n                    <jats:sup>high<\/jats:sup>\n                    FOXP3\n                    <jats:sup>+<\/jats:sup>\n                    regulatory T-cells (T\n                    <jats:sub>regs<\/jats:sub>\n                    ) from 51 patients with non\u2013small-cell lung cancer (NSCLC) and 33 healthy donors. Regulatory T-cells were identified by fluorescence-activated cell sorting by using a panel of antibodies and by reverse transcriptase polymerase chain reaction analysis for FOXP3 expression. Functional studies were done to analyze their inhibitory role. Finally, regulatory T-cells were analyzed in malignant pleura effusion (PE) from patients with NSCLC. Patients with NSCLC have increased numbers of CD4\n                    <jats:sup>+<\/jats:sup>\n                    CD25\n                    <jats:sup>high<\/jats:sup>\n                    FOXP3\n                    <jats:sup>+<\/jats:sup>\n                    T\n                    <jats:sub>regs<\/jats:sub>\n                    in their peripheral blood and pleura effusion (PE), which express high levels of CTLA-4, GITR. These cells were anergic toward T-cell receptor stimulation and, when cocultured with activated CD4\n                    <jats:sup>+<\/jats:sup>\n                    CD25\n                    <jats:sup>\u2212<\/jats:sup>\n                    cells, potently suppressed their proliferation and cytokine secretion. Our data suggest that in NSCLC patients, there is an increase of CD4\n                    <jats:sup>+<\/jats:sup>\n                    CD25\n                    <jats:sup>high<\/jats:sup>\n                    FOXP3\n                    <jats:sup>+<\/jats:sup>\n                    regulatory T-cells in the peripheral blood and tumor microenvironment. These T-cells might prevent effective antitumor immune responses, and the increase in frequency of CD4\n                    <jats:sup>+<\/jats:sup>\n                    CD25\n                    <jats:sup>high<\/jats:sup>\n                    FOXP3\n                    <jats:sup>+<\/jats:sup>\n                    Tregs might play a role in the modulation of the immune response against NSCLC and could be important in the design of immunotherapeutic approaches.\n                  <\/jats:p>","DOI":"10.1089\/cbr.2008.0612","type":"journal-article","created":{"date-parts":[[2009,6,19]],"date-time":"2009-06-19T10:37:09Z","timestamp":1245407829000},"page":"357-367","source":"Crossref","is-referenced-by-count":35,"title":["The Prevalence of FOXP3\n                    <sup>+<\/sup>\n                    Regulatory T-Cells in Peripheral Blood of Patients with NSCLC"],"prefix":"10.1177","volume":"24","author":[{"given":"Liu","family":"Li","sequence":"first","affiliation":[{"name":"Hua-Zhong University of Science and Technology","place":["People's of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiu Guo","family":"Chao","sequence":"additional","affiliation":[{"name":"Hua-Zhong University of Science and Technology","place":["People's of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liu Zhong","family":"Ping","sequence":"additional","affiliation":[{"name":"Hua-Zhong University of Science and Technology","place":["People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Xue","sequence":"additional","affiliation":[{"name":"Hua-Zhong University of Science and Technology","place":["People's of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhao Yan","family":"Xia","sequence":"additional","affiliation":[{"name":"Hua-Zhong University of Science and Technology","place":["People's of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ding","family":"Qian","sequence":"additional","affiliation":[{"name":"Hua-Zhong University of Science and Technology","place":["People's of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huang","family":"Shi-ang","sequence":"additional","affiliation":[{"name":"Hua-Zhong University of Science and Technology","place":["People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2009,6,1]]},"container-title":["Cancer Biotherapy and Radiopharmaceuticals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/cbr.2008.0612","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/cbr.2008.0612","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T14:12:13Z","timestamp":1777385533000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/cbr.2008.0612"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,6]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2009,6]]}},"alternative-id":["10.1089\/cbr.2008.0612"],"URL":"https:\/\/doi.org\/10.1089\/cbr.2008.0612","relation":{},"ISSN":["1084-9785","1557-8852"],"issn-type":[{"value":"1084-9785","type":"print"},{"value":"1557-8852","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,6]]}}}