{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T16:55:40Z","timestamp":1777481740934,"version":"3.51.4"},"reference-count":19,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2012,3,1]],"date-time":"2012-03-01T00:00:00Z","timestamp":1330560000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Biotherapy and Radiopharmaceuticals"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:p>\n                    Intact immunoglobulin G antibody has a relatively large molecule size of approximately 150\u2009kDa that remains in the bloodstream for many weeks, which is a considerable disadvantage when it is used to carry radioactive materials for imaging. To lower background activity and increase the contrast of images, we investigated antivascular endothelial growth factor (VEGF) receptor 2 antibody (DC101) conjugated dextran for VEGF receptor 2 imaging in tumor xenografted mice. DTPA-conjugated aminodextran was synthesized, reacted with sulfo-LC-SPDP, and then reacted with DC101. The binding affinity of DTPA-dextran-DC101 to Flk-1 was measured. The gamma imaging and biodistributions of\n                    <jats:sup>99m<\/jats:sup>\n                    Tc-DTPA-dextran-DC101,\n                    <jats:sup>99m<\/jats:sup>\n                    Tc-DTPA-DC101, and\n                    <jats:sup>125<\/jats:sup>\n                    I-DC101 were studied in B16F10 melanoma xenografted mice. The dissociation values for DC101, DTPA-DC101, and DTPA-dextran-DC101 were 22.48, 3.05, and 14.74 pM, respectively. In gamma images,\n                    <jats:sup>99m<\/jats:sup>\n                    Tc-DTPA-dextran-DC101 showed weak liver uptake and rapid kidney elimination. In biodistribution results, the liver uptake of\n                    <jats:sup>99m<\/jats:sup>\n                    Tc-DTPA-dextran-DC101 was similar with that of\n                    <jats:sup>99m<\/jats:sup>\n                    Tc-DTPA-DC101 at each time point. However, the blood activity of\n                    <jats:sup>99m<\/jats:sup>\n                    Tc-DTPA-dextran-DC101 has shown significant differences, compared with\n                    <jats:sup>99m<\/jats:sup>\n                    Tc-DTPA-DC101 at all time points. The tumor accumulation of dextran-conjugated antibody was increased with time, whereas that of dextran nonconjugated antibody decreased. In particular, the pattern of tumor uptake of\n                    <jats:sup>99m<\/jats:sup>\n                    Tc-DTPA-dextran-DC101 was similar to that of\n                    <jats:sup>125<\/jats:sup>\n                    I-DC101, so this was thought to reflect the kinetics of DC101, unlike the nonconjugated form. The results of this study suggested that introduction of dextran moiety to make\n                    <jats:sup>99m<\/jats:sup>\n                    Tc-radiolabeled DC101 imaging agent could provide better images with the impaired background and the steady increasing binding to the receptor. However, further studies are necessary to improve clinical pharmacokinetics, such as enhancement of tumor uptake and impaired renal uptake.\n                  <\/jats:p>","DOI":"10.1089\/cbr.2011.0977","type":"journal-article","created":{"date-parts":[[2011,12,7]],"date-time":"2011-12-07T15:24:07Z","timestamp":1323271447000},"page":"141-148","source":"Crossref","is-referenced-by-count":2,"title":["Dextran-Conjugated Vascular Endothelial Growth Factor Receptor Antibody for\n                    <i>In Vivo<\/i>\n                    Melanoma Xenografted Mouse Imaging"],"prefix":"10.1177","volume":"27","author":[{"given":"Eun-Mi","family":"Kim","sequence":"first","affiliation":[{"name":"Chonbuk National University Medical School and Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hwan-Jeong","family":"Jeong","sequence":"additional","affiliation":[{"name":"Chonbuk National University Medical School and Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min-Hee","family":"Jeong","sequence":"additional","affiliation":[{"name":"Chonbuk National University Medical School and Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang-Moon","family":"Lee","sequence":"additional","affiliation":[{"name":"Chonbuk National University Medical School and Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Su-Jin","family":"Cheong","sequence":"additional","affiliation":[{"name":"Chonbuk National University Medical School and Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong Wook","family":"Kim","sequence":"additional","affiliation":[{"name":"Chonbuk National University Medical School and Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seok Tae","family":"Lim","sequence":"additional","affiliation":[{"name":"Chonbuk National University Medical School and Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myung-Hee","family":"Sohn","sequence":"additional","affiliation":[{"name":"Chonbuk National University Medical School and Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2012,3,1]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2005.03.8471"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-1004-0009"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1142"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1322"},{"key":"e_1_3_3_6_2","first-page":"1683","article-title":"Radiation dosimetry of 99mTc-labeled C225 in patients with squamous cell carcinoma of the head and neck","volume":"45","author":"Schechter NR","year":"2004","unstructured":"Schechter NR, , Wendt RE3rd, , Yang DJ et al. Radiation dosimetry of 99mTc-labeled C225 in patients with squamous cell carcinoma of the head and neck. J Nucl Med, 2004; 45:1683.","journal-title":"J Nucl Med"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-8051(02)00322-0"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/bc0001443"},{"key":"e_1_3_3_9_2","first-page":"1530","article-title":"Conjugation with (111)In-DTPA-poly(ethylene glycol) improves imaging of anti-EGF receptor antibody C225","volume":"42","author":"Wen X","year":"2001","unstructured":"Wen X, , Wu QP, , Ke S et al. Conjugation with (111)In-DTPA-poly(ethylene glycol) improves imaging of anti-EGF receptor antibody C225. J Nucl Med, 2001; 42:1530.","journal-title":"J Nucl Med"},{"key":"e_1_3_3_10_2","first-page":"868","article-title":"Imaging experimental atherosclerotic lesions in ApoE knockout mice: Enhanced targeting with Z2D3-anti-DTPA bispecific antibody and 99mTc-labeled negatively charged polymers","volume":"47","author":"Khaw BA","year":"2006","unstructured":"Khaw BA, , Tekabe Y, , Johnson LL. Imaging experimental atherosclerotic lesions in ApoE knockout mice: Enhanced targeting with Z2D3-anti-DTPA bispecific antibody and 99mTc-labeled negatively charged polymers. J Nucl Med, 2006; 47:868.","journal-title":"J Nucl Med"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-409X(00)00134-4"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/jps.20548"},{"key":"e_1_3_3_13_2","first-page":"1264","article-title":"Reduction of background activity through radiolabeling of antifibrin Fab' with 99mTc-dextran","volume":"1","author":"Line BR","year":"2000","unstructured":"Line BR, , Weber PB, , Lukasiewicz R et al. Reduction of background activity through radiolabeling of antifibrin Fab' with 99mTc-dextran. J Nucl Med, 2000; 1:1264.","journal-title":"J Nucl Med"},{"key":"e_1_3_3_14_2","first-page":"5209","article-title":"Antivascular endothelial growth factor receptor (fetal liver kinase 1) monoclonal antibody inhibits tumor angiogenesis and growth of several mouse and human tumors","volume":"59","author":"Prewett M","year":"1999","unstructured":"Prewett M, , Huber J, , Li Y et al. Antivascular endothelial growth factor receptor (fetal liver kinase 1) monoclonal antibody inhibits tumor angiogenesis and growth of several mouse and human tumors. Cancer Res, 1999; 59:5209.","journal-title":"Cancer Res"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.32470"},{"key":"e_1_3_3_16_2","first-page":"2664","article-title":"Monoclonal antibody-mediated drug targeting to choroidal neovascularization in the rat","volume":"42","author":"Kamizuru H","year":"2001","unstructured":"Kamizuru H, , Kimura H, , Yasukawa T et al. Monoclonal antibody-mediated drug targeting to choroidal neovascularization in the rat. 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