{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T20:29:19Z","timestamp":1791404959999,"version":"4.3.3"},"reference-count":46,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T00:00:00Z","timestamp":1270080000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T00:00:00Z","timestamp":1270080000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cellular Reprogramming"],"published-print":{"date-parts":[[2010,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Successful somatic cell nuclear transfer (SCNT) requires epigenetic reprogramming of a differentiated donor cell nucleus. Incorrect reprogramming of epigenetic markings such as DNA methylation is associated with compromised prenatal development and postnatal abnormalities. Clones that survive into adulthood, in contrast, are assumed to possess a normalized epigenome corresponding to their normal phenotype. To address this point, we used capillary electrophoresis to measure 5-methylcytosine (5mC) levels in leukocyte DNA of 38 healthy female bovine clones that represented five genotypes from the Simmental breed and four genotypes from the Holstein breed. The estimated variance in 5mC level\n                    <jats:italic toggle=\"yes\">within<\/jats:italic>\n                    clone genotypes of both breeds [0.104, 95% confidence interval (CI): 0.070\u20130.168] was higher than\n                    <jats:italic toggle=\"yes\">between<\/jats:italic>\n                    clone genotypes (0, CI: 0\u20130.047). We quantified the contribution of SCNT to this unexpected variability by comparing the 19 Simmental clones with 12 female Simmental monozygotic twin pairs of similar age. In Simmental clones, the estimated variability\n                    <jats:italic toggle=\"yes\">within<\/jats:italic>\n                    genotype (0.0636, CI: 0.0358\u20130.127) was clearly higher than in twin pairs (0.0091, CI: 0.0047\u20130.0229). In clones, variability\n                    <jats:italic toggle=\"yes\">within<\/jats:italic>\n                    genotype (0.0636) was again higher than\n                    <jats:italic toggle=\"yes\">between<\/jats:italic>\n                    genotypes (0, CI: 0\u20130.077). Twins, in contrast, showed lower variability\n                    <jats:italic toggle=\"yes\">within<\/jats:italic>\n                    genotypes (0.0091) than\n                    <jats:italic toggle=\"yes\">between<\/jats:italic>\n                    genotypes (0.0136, CI: 0.00250\u20130.0428). Importantly, the absolute deviations of 5mC values of individual SCNT clones from their genotype means were fivefold increased in comparison to twins. Further comparisons with noncloned controls revealed DNA hypermethylation in most of the clones. The clone-specific variability in DNA methylation and DNA hypermethylation clearly show that healthy adult SCNT clones must be considered as epigenome variants.\n                  <\/jats:p>","DOI":"10.1089\/cell.2009.0062","type":"journal-article","created":{"date-parts":[[2010,4,16]],"date-time":"2010-04-16T10:05:25Z","timestamp":1271412325000},"page":"175-181","source":"Crossref","is-referenced-by-count":22,"title":["Quantification of Leukocyte Genomic 5-Methylcytosine Levels Reveals Epigenetic Plasticity in Healthy Adult Cloned Cattle"],"prefix":"10.1177","volume":"12","author":[{"given":"B\u00e9atrice","family":"de Montera","sequence":"first","affiliation":[{"name":"INRA","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dalia","family":"El Zeihery","sequence":"additional","affiliation":[{"name":"University of Wuppertal","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sigrid","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Chair for Molecular Animal Breeding and Biotechnology, and Laboratory for Functional Genome Analysis (LAFUGA)","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9l\u00e8ne","family":"Jammes","sequence":"additional","affiliation":[{"name":"INRA","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gottfried","family":"Brem","sequence":"additional","affiliation":[{"name":"University of Veterinary Sciences","place":["Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Horst-Dieter","family":"Reichenbach","sequence":"additional","affiliation":[{"name":"Bayerische Landesanstalt f\u00fcr Landwirtschaft","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabian","family":"Scheipl","sequence":"additional","affiliation":[{"name":"STABLAB","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascale","family":"Chavatte-Palmer","sequence":"additional","affiliation":[{"name":"INRA","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeri","family":"Zakhartchenko","sequence":"additional","affiliation":[{"name":"Chair for Molecular Animal Breeding and Biotechnology, and Laboratory for Functional Genome Analysis (LAFUGA)","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver J.","family":"Schmitz","sequence":"additional","affiliation":[{"name":"University of Wuppertal","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eckhard","family":"Wolf","sequence":"additional","affiliation":[{"name":"Chair for Molecular Animal Breeding and Biotechnology, and Laboratory for Functional Genome Analysis (LAFUGA)","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Paul","family":"Renard","sequence":"additional","affiliation":[{"name":"INRA","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Hiendleder","sequence":"additional","affiliation":[{"name":"Chair for Molecular Animal Breeding and Biotechnology, and Laboratory for Functional Genome Analysis (LAFUGA)","place":["Germany"]},{"name":"The University of Adelaide","place":["Australia"]},{"name":"The University of Adelaide","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2010,4,16]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.4161\/epi.2.1.4066"},{"key":"e_1_3_3_3_1","unstructured":"Bates D. 2009. lme4: linear mixed-effects models using S4 classes. 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