{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:45:55Z","timestamp":1777509955257,"version":"3.51.4"},"reference-count":39,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2010,6,1]],"date-time":"2010-06-01T00:00:00Z","timestamp":1275350400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cellular Reprogramming"],"published-print":{"date-parts":[[2010,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Mutations in\n                    <jats:italic toggle=\"yes\">COL1A1<\/jats:italic>\n                    or\n                    <jats:italic toggle=\"yes\">COL1A2<\/jats:italic>\n                    genes lead to osteogenesis Imperfecta (OI) in humans. There are three possiblities to successfully treat OI including (1) gene therapy, (2) mesenchymal stem cell (MSC) therapy, or (3) a combination of both. The aim of this study was to develop a model for combined gene\/cell OI therapy by targeting\n                    <jats:italic toggle=\"yes\">Col1a1<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">Col1a2<\/jats:italic>\n                    genes with isogenic sequences from corresponding human genes in rat bone marrow (BM)-derived MSCs. The recombination efficacy was tested for five different rat\u2013human\u2013rat hybrid DNAs with rat fragments that were 1 to 4\u2009kb long. For selection of transfected clones a neomycine resistance gene was cotransfected, and clones resistant to G418 (G418\n                    <jats:sup>+<\/jats:sup>\n                    ) were recovered and screened for integration of specific gene loci in the rat genome. Over 90% of G418\n                    <jats:sup>+<\/jats:sup>\n                    clones correctly integrated the rat\u2013human\u2013rat hybrid DNAs, and both OI loci in the rat genome were targeted to a similar degree. Longer homologous sequences integrated into rat collagen genes \u223c10 times more efficiently. Based on our data the nonviral gene targeting technology could be potentially employed to repair collagen genes in OI patients.\n                  <\/jats:p>","DOI":"10.1089\/cell.2009.0084","type":"journal-article","created":{"date-parts":[[2010,6,30]],"date-time":"2010-06-30T23:04:44Z","timestamp":1277939084000},"page":"275-282","source":"Crossref","is-referenced-by-count":10,"title":["Optimization of Genetic Engineering and Homologous Recombination of Collagen Type I Genes in Rat Bone Marrow Mesenchymal Stem Cells (MSC)"],"prefix":"10.1177","volume":"12","author":[{"given":"Maciej","family":"Tarnowski","sequence":"first","affiliation":[{"name":"Medical University of Silesia","place":["Poland"]},{"name":"CoE for Teaching and Research of Molecular Biology of Matrix and Nanotechnology"},{"name":"University of Louisville"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Szyd\u0142o","sequence":"additional","affiliation":[{"name":"Medical University of 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