{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:58:26Z","timestamp":1777510706499,"version":"3.51.4"},"reference-count":61,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Cellular Reprogramming"],"published-print":{"date-parts":[[2019,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The embryonic stem cell line derivation from nonpermissive mouse strains is a challenging and highly inefficient process. The cellular reprogramming strategy provides an alternative route for generating pluripotent stem cell (PSC) lines from such strains. In this study, we successfully derived an enhanced green fluorescent protein (EGFP)-transgenic \u201cN9\u201d induced pluripotent stem cell (iPS cell, iPSC) line from the FVB\/N strain-derived mouse embryonic fibroblasts (MEFs). The exposure of MEFs to human\n                    <jats:italic toggle=\"yes\">OCT4<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">SOX2<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">KLF4<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">c-MYC<\/jats:italic>\n                    (OSKM) transgenes via lentiviral transduction resulted in complete reprogramming. The N9 iPS cell line demonstrated all the criteria of a typical mouse PSC line, including normal colony morphology and karyotype (40,XY), high replication and propagation efficiencies, expression of the pluripotency-associated genes, spontaneous differentiation to three germ lineage-derived cell types, and robust potential of chimeric blastocyst formation. Taken together, using human OSKM genes for transduction, we report, for the first time, the successful derivation of an EGFP-expressing iPS cell line from a genetically nonpermissive transgenic FVB\/N mouse. This cell line could provide opportunities for designing protocols for efficient derivation of PSC lines from other nonpermissive strains and developing mouse models of human diseases.\n                  <\/jats:p>","DOI":"10.1089\/cell.2019.0019","type":"journal-article","created":{"date-parts":[[2019,10,9]],"date-time":"2019-10-09T15:57:26Z","timestamp":1570636646000},"page":"270-284","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Successful Derivation of an Induced Pluripotent Stem Cell Line from a Genetically Nonpermissive Enhanced Green Fluorescent Protein-Transgenic FVB\/N Mouse Strain"],"prefix":"10.1177","volume":"21","author":[{"given":"Deepti","family":"Abbey","sequence":"first","affiliation":[{"name":"Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore, India."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gurbind","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore, India."},{"name":"Present address: Centre for Stem Cell Research, Christian Medical College Campus, Bagayam, Vellore, India."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isha","family":"Verma","sequence":"additional","affiliation":[{"name":"Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore, India."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suchitra","family":"Derebail","sequence":"additional","affiliation":[{"name":"Thermo Fisher Scientific, Frederick, Maryland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Udaykumar","family":"Kolkundkar","sequence":"additional","affiliation":[{"name":"Thermo Fisher Scientific, Frederick, Maryland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darshan S.","family":"Chandrashekar","sequence":"additional","affiliation":[{"name":"Institute of Bioinformatics and Applied Biotechnology, Bangalore, India."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kshitish K.","family":"Acharya","sequence":"additional","affiliation":[{"name":"Institute of Bioinformatics and Applied Biotechnology, Bangalore, India."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohan C.","family":"Vemuri","sequence":"additional","affiliation":[{"name":"Thermo Fisher Scientific, Frederick, Maryland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Polani B.","family":"Seshagiri","sequence":"additional","affiliation":[{"name":"Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore, India."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2019,10,9]]},"reference":[{"key":"e_1_3_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diff.2017.04.001"},{"key":"e_1_3_6_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.11.5709"},{"key":"e_1_3_6_4_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.102.006197"},{"key":"e_1_3_6_5_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2003.1327"},{"key":"e_1_3_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-008-1727-4"},{"key":"e_1_3_6_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-02-269589"},{"key":"e_1_3_6_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-213X-8-57"},{"key":"e_1_3_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(95)00685-0"},{"key":"e_1_3_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01973986"},{"key":"e_1_3_6_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2011.0427"},{"key":"e_1_3_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.10306"},{"key":"e_1_3_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(03)00483-2"},{"key":"e_1_3_6_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq405"},{"key":"e_1_3_6_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/292154a0"},{"key":"e_1_3_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2006.09.003"},{"key":"e_1_3_6_17_1","doi-asserted-by":"publisher","DOI":"10.2183\/pjab.85.348"},{"key":"e_1_3_6_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-32116-8"},{"key":"e_1_3_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20594"},{"key":"e_1_3_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2009.04.015"},{"key":"e_1_3_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-472X(02)01021-4"},{"key":"e_1_3_6_22_1","first-page":"189","article-title":"In vitro manipulation of preimplantation embryos","author":"Hogan B.","year":"1994","unstructured":"Hogan B., , Beddington R., , Costantini F., , and Lacy E. 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