{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:59:12Z","timestamp":1777510752560,"version":"3.51.4"},"reference-count":71,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Cellular Reprogramming"],"published-print":{"date-parts":[[2020,12,1]]},"abstract":"<jats:p>Equine endometrial and adipose mesenchymal stem cells (eMSCs and aMSCs, respectively) were isolated from the same donors of thoroughbred mares. The cells displayed characteristic features of MSCs, including trilineage mesodermal and also neurogenic differentiation. We evaluated the influence of cellular origin on their transcriptome profile. Cellular RNA was isolated and sequenced and extracellular vesicles (EVs) were obtained from conditioned medium of cells cultured in medium depleted of EVs, and their microRNA (miRNA) cargo analyzed by sequencing. Differential expression of mRNAs and EV-miRNA was analyzed, as well as pathways and processes most represented in each cell origin. mRNA reads from all expressed genes clustered according to the cellular origin. A total of 125 up- and 51 downregulated genes were identified and 31 differentially expressed miRNAs. Based on mRNA sequencing, endometrial MSCs strongly upregulated genes involved in the Hippo, transforming growth factor beta, and pluripotency signaling pathways. Alongside with this, pathways involved in extracellular matrix reorganization were the most represented in the miRNA cargo of EVs secreted by eMSCs. The niche from which MSCs originated defined the transcriptomic signature of the cells, including the secretion of lineage-specific loaded EV to ensure proper communication and homeostasis. Identification and testing their biological functions can provide new tools for the therapeutic use of horse MSC.<\/jats:p>","DOI":"10.1089\/cell.2020.0026","type":"journal-article","created":{"date-parts":[[2020,9,29]],"date-time":"2020-09-29T14:52:56Z","timestamp":1601391176000},"page":"311-327","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Distinctive Cellular Transcriptomic Signature and MicroRNA Cargo of Extracellular Vesicles of Horse Adipose and Endometrial Mesenchymal Stem Cells from the Same Donors"],"prefix":"10.1177","volume":"22","author":[{"given":"Felipe","family":"Navarrete","sequence":"first","affiliation":[{"name":"Department of Animal Science, Faculty of Veterinary Sciences, Universidad de Concepci\u00f3n, Chillan, Chile."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yat Sen","family":"Wong","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Faculty of Veterinary Sciences, Universidad de Concepci\u00f3n, Chillan, Chile."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joel","family":"Cabezas","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Faculty of Veterinary Sciences, Universidad de Concepci\u00f3n, Chillan, Chile."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gonzalo","family":"Riadi","sequence":"additional","affiliation":[{"name":"Center for Bioinformatics, Simulation and Modeling (CBSM), Department of Bioinformatics, Faculty of Engineering, University of Talca, Talca, Chile."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"Manr\u00edquez","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Faculty of Veterinary Sciences, Universidad de Concepci\u00f3n, Chillan, Chile."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela","family":"Rojas","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Faculty of Veterinary Sciences, Universidad de Concepci\u00f3n, Chillan, Chile."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Carolina","family":"Furlanetto Man\u00e7anares","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Faculty of Veterinary Sciences, Universidad de Concepci\u00f3n, Chillan, Chile."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lleretny","family":"Rodriguez-Alvarez","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Faculty of Veterinary Sciences, Universidad de Concepci\u00f3n, Chillan, Chile."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Saravia","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Faculty of Veterinary Sciences, Universidad de Concepci\u00f3n, Chillan, Chile."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fidel Ovidio","family":"Castro","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Faculty of Veterinary Sciences, Universidad de Concepci\u00f3n, Chillan, Chile."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2020,12,2]]},"reference":[{"key":"e_1_3_5_2_1","doi-asserted-by":"publisher","DOI":"10.2746\/042516400776563554"},{"key":"e_1_3_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tice.2013.05.002"},{"key":"e_1_3_5_4_1","first-page":"21","article-title":"sRNAbench: Profiling of small RNAs and its sequence variants in single or multi-species high-throughput experiments","volume":"1","author":"Barturen G.","year":"2014","unstructured":"Barturen G., , Rueda A., , Hamberg M., , Alganza A., , Lebron R., , Kotsyfakis M., , Shi B.J, , Koppers-Lalic D., , and Hackenberg M. (2014). sRNAbench: Profiling of small RNAs and its sequence variants in single or multi-species high-throughput experiments. Methods Next Generation Seq. 1, 21\u201331.","journal-title":"Methods Next Generation Seq"},{"key":"e_1_3_5_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfs168"},{"key":"e_1_3_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.2010.00098.x"},{"key":"e_1_3_5_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19020435"},{"key":"e_1_3_5_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033115"},{"key":"e_1_3_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2017.09.035"},{"key":"e_1_3_5_10_1","first-page":"4957806","article-title":"Equine adipose-derived mesenchymal stromal cells release extracellular vesicles enclosing different subsets of small RNAs","author":"Capomaccio E.","year":"2019","unstructured":"Capomaccio E., , Cappelli K., , Bazzucchi C., , Coletti M., , Gialletti R., , Moriconi F., , Passamonti F., , Pepe M., , Petrini S., , Mecocci S., , Silvestrelli M., , and Pascucci L. (2019). Equine adipose-derived mesenchymal stromal cells release extracellular vesicles enclosing different subsets of small RNAs. Stem Cells Int. 2019, 4957806.","journal-title":"Stem Cells Int. 2019,"},{"key":"e_1_3_5_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-016-2504-9"},{"key":"e_1_3_5_12_1","first-page":"207","article-title":"Immunomodulation by mesenchymal stem cells in veterinary species","volume":"63","author":"Carrade D.D.","year":"2013","unstructured":"Carrade D.D., , and Borjesson D.L. (2013). Immunomodulation by mesenchymal stem cells in veterinary species. Comp. Med. 63, 207\u2013217.","journal-title":"Comp. Med"},{"key":"e_1_3_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2013.12.005"},{"key":"e_1_3_5_14_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.103.024109"},{"key":"e_1_3_5_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-21464-0"},{"key":"e_1_3_5_16_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-12-0437"},{"key":"e_1_3_5_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2010.485"},{"key":"e_1_3_5_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep19386"},{"key":"e_1_3_5_19_1","first-page":"315","article-title":"Minimal criteria for defining multipotent mesenchymal stromal cells","volume":"8","author":"Dominici M.","year":"2006","unstructured":"Dominici M., , Le Blanc K., , Mueller I., , Slaper-Cortenbach I., , Marini F.C., , Krause D.S., , Deans R., , Keating A., , Prockop D, .j. and Horwitz E.m. (2006). Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy, 8, 315\u2013317.","journal-title":"The International Society for Cellular Therapy position statement. Cytotherapy"},{"key":"e_1_3_5_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/1878-0261.12527"},{"key":"e_1_3_5_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-6-65"},{"key":"e_1_3_5_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-10-154"},{"key":"e_1_3_5_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12031-013-9957-z"},{"key":"e_1_3_5_24_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.1998.59.09.1182"},{"key":"e_1_3_5_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1205951109"},{"key":"e_1_3_5_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0810584105"},{"key":"e_1_3_5_27_1","first-page":"377","article-title":"Endometrial stem cells","volume":"19","author":"Gargett C.E.","year":"2007","unstructured":"Gargett C.E., , Chana R., , and Schwaba K. (2007). Endometrial stem cells. Obstet. Gynecol. 19, 377\u2013383.","journal-title":"Obstet. Gynecol"},{"key":"e_1_3_5_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gaq061"},{"key":"e_1_3_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2012.05.004"},{"key":"e_1_3_5_30_1","doi-asserted-by":"publisher","DOI":"10.1089\/cell.2015.0040"},{"key":"e_1_3_5_31_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.529115"},{"key":"e_1_3_5_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-015-1262-5"},{"key":"e_1_3_5_33_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.70.723"},{"key":"e_1_3_5_34_1","doi-asserted-by":"publisher","DOI":"10.1097\/WNR.0b013e328344a666"},{"key":"e_1_3_5_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12185-012-1067-0"},{"key":"e_1_3_5_36_1","doi-asserted-by":"crossref","first-page":"e0175986","DOI":"10.1371\/journal.pone.0175986","article-title":"Niche matters: the comparison between bone marrow stem cells and endometrial stem cells and stromal fibroblasts reveal distinct migration and cytokine profiles in response to inflammatory stimulus","author":"Khatun M.","year":"2017","unstructured":"Khatun M., , Sorjamaa A., , Kangasniemi M., , Sutinen M., , Salo T., , Liakka A., , Lehenkari P., , Tapanainen J.S., , Vuolteenaho O., , Chen J.C., , Lehtonen S., , and Piltonen T.T. (2017). Niche matters: the comparison between bone marrow stem cells and endometrial stem cells and stromal fibroblasts reveal distinct migration and cytokine profiles in response to inflammatory stimulus. PLoS One 12, e0175986.","journal-title":"PLoS One 12,"},{"key":"e_1_3_5_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7007-10-19"},{"key":"e_1_3_5_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/term.465"},{"key":"e_1_3_5_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/evj.12052"},{"key":"e_1_3_5_40_1","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering5030075"},{"key":"e_1_3_5_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12969"},{"key":"e_1_3_5_42_1","doi-asserted-by":"publisher","DOI":"10.3727\/000000007783465271"},{"key":"e_1_3_5_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-019-1285-y"},{"key":"e_1_3_5_44_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI61209"},{"key":"e_1_3_5_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13208"},{"key":"e_1_3_5_46_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1200822"},{"key":"e_1_3_5_47_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0111533"},{"key":"e_1_3_5_48_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-11-0202"},{"key":"e_1_3_5_49_1","doi-asserted-by":"publisher","DOI":"10.1097\/PRS.0b013e318180ec9f"},{"key":"e_1_3_5_50_1","first-page":"e194","article-title":"Mir-34: a new weapon against cancer? Mol","author":"Misso G.","year":"2014","unstructured":"Misso G., , Di Martino M.T., , De Rosa G., , Farooqi A.A., , Lombardi A., , Campani V., , Zarone M.R., , Gull\u00e0 A., , Tagliaferri P., , Tassone P., , and Caraglia M. (2014). Mir-34: a new weapon against cancer? Mol. Ther. Nucleic Acids 3, e194.","journal-title":"Ther. Nucleic Acids 3,"},{"key":"e_1_3_5_51_1","first-page":"101","article-title":"Role of TGF-\u03b2s in normal human endometrium and endometriosis, Hum","volume":"25","author":"Omwandho C.O.A.","year":"2010","unstructured":"Omwandho C.O.A., , Konrad L., , Halis G., , Oehmke F., , and Tinneberg H.R. (2010). Role of TGF-\u03b2s in normal human endometrium and endometriosis, Hum. Reprod. 25, 101\u2013109.","journal-title":"Reprod"},{"key":"e_1_3_5_52_1","first-page":"doi","article-title":"miR-34a aggravates obesity-induced adipose inflammation and metabolic dysfunction via blocking polarization of anti-inflammatory M2 macrophage","author":"Pan Y.","year":"2018","unstructured":"Pan Y., , Hui H., , Hoo R., , Feng T., , Lam K.S., , and Xu A. (2018). miR-34a aggravates obesity-induced adipose inflammation and metabolic dysfunction via blocking polarization of anti-inflammatory M2 macrophage. Diabetes 67 (Abstract Meeting). DOI: doi.org\/10.2337\/db18-2025-P","journal-title":"Diabetes 67 (Abstract Meeting)"},{"key":"e_1_3_5_53_1","first-page":"61","article-title":"Potential clinical applications of adult human mesenchymal stem cell (Prochymal\u00ae) therapy","volume":"4","author":"Patel A.N.","year":"2011","unstructured":"Patel A.N., , and Genovese J. (2011). Potential clinical applications of adult human mesenchymal stem cell (Prochymal\u00ae) therapy. Stem Cells Cloning, 4, 61\u201372.","journal-title":"Stem Cells Cloning"},{"key":"e_1_3_5_54_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-016-0429-6"},{"key":"e_1_3_5_55_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.083691"},{"key":"e_1_3_5_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4644(19991201)75:3<424::AID-JCB8>3.0.CO;2-8"},{"key":"e_1_3_5_57_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-016-0214-7"},{"key":"e_1_3_5_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-00629-3"},{"key":"e_1_3_5_59_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-017-0616-0"},{"key":"e_1_3_5_60_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/3816256"},{"key":"e_1_3_5_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mehy.2011.02.033"},{"key":"e_1_3_5_62_1","first-page":"1331","article-title":"MicroRNA-380-5p inhibits migration and invasion of ovarian cancer SKOV3 cells and regulates epithelial-mesenchymal transition factors","volume":"10","author":"Su H.","year":"2017","unstructured":"Su H., , Liu D., , Yan C., , and Wang G. (2017). MicroRNA-380-5p inhibits migration and invasion of ovarian cancer SKOV3 cells and regulates epithelial-mesenchymal transition factors. Int. J. Clin. Exp. Pathol. 10, 1331\u20131339.","journal-title":"Int. J. Clin. Exp. Pathol"},{"key":"e_1_3_5_63_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv403"},{"key":"e_1_3_5_64_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.10365"},{"key":"e_1_3_5_65_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4547(20000815)61:4<364::AID-JNR2>3.0.CO;2-C"},{"key":"e_1_3_5_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2013.11.036"},{"key":"e_1_3_5_67_1","first-page":"133e41","article-title":"Osteogenic differentiation of rat mesenchymal stem cells from adipose tissue in comparison with bone marrow mesenchymal stem cells: Melatonin as a differentiation factor","author":"Zaminy A.","year":"2008","unstructured":"Zaminy A., , Ragerdi-Kashani I., , Barbarestani M., , Hedayatpour A., , Mahmoudi R., , and Farzaneh-Nejad A. (2008). Osteogenic differentiation of rat mesenchymal stem cells from adipose tissue in comparison with bone marrow mesenchymal stem cells: Melatonin as a differentiation factor. Iran Biomed. J. 12, 133e41.","journal-title":"Iran Biomed. J. 12,"},{"key":"e_1_3_5_68_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.3652"},{"key":"e_1_3_5_69_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-019-1059-5"},{"key":"e_1_3_5_70_1","doi-asserted-by":"publisher","DOI":"10.4103\/0366-6999.218013"},{"key":"e_1_3_5_71_1","doi-asserted-by":"publisher","DOI":"10.3109\/09513590.2015.1069268"},{"key":"e_1_3_5_72_1","doi-asserted-by":"publisher","DOI":"10.2147\/CMAR.S168594"}],"container-title":["Cellular Reprogramming"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/cell.2020.0026","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/cell.2020.0026","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/cell.2020.0026","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T15:37:57Z","timestamp":1777390677000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/cell.2020.0026"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,1]]},"references-count":71,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2020,12,1]]}},"alternative-id":["10.1089\/cell.2020.0026"],"URL":"https:\/\/doi.org\/10.1089\/cell.2020.0026","relation":{},"ISSN":["2152-4971","2152-4998"],"issn-type":[{"value":"2152-4971","type":"print"},{"value":"2152-4998","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,12,1]]}}}