{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:59:12Z","timestamp":1777510752725,"version":"3.51.4"},"reference-count":15,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Cellular Reprogramming"],"published-print":{"date-parts":[[2020,12,1]]},"abstract":"\n Oxidative stress can impede maturation of the nucleus and cytoplasm of oocytes during\n in vitro<\/jats:italic>\n maturation (IVM).\n Rhodiola sachalinensis<\/jats:italic>\n , an herb commonly used in traditional Chinese medicine, conveys antioxidative effects to cryopreserved bovine sperm. Therefore, the aims of this study were to evaluate the effects of different concentrations of\n R. sachalinensis<\/jats:italic>\n aqueous extract (RSAE) on IVM and subsequent\n in vitro<\/jats:italic>\n embryonic development after parthenogenetic activation (PA),\n in vitro<\/jats:italic>\n fertilization (IVF), and somatic cell nuclear transfer (SCNT). The results showed that RSAE supplementation (6 and 60\u2009mg\/L) significantly increased intracellular glutathione levels, but had no effect on maturation rates or reactive oxygen species. After\n in vitro<\/jats:italic>\n culture, greater blastocyst formation was observed in PA embryos (6\u2009mg\/L RSAE), as well as in IVF and SCNT embryos (60\u2009mg\/L) matured in RSAE-supplemented IVM media. In conclusion, although there was no significant improvement in the maturation rate, RSAE supplementation conveyed an antioxidative effect during IVM, and improved subsequent embryonic development\n in vitro<\/jats:italic>\n . Further studies are needed to explore gene expression pattern in oocytes and embryos treated with RSAE.\n <\/jats:p>","DOI":"10.1089\/cell.2020.0041","type":"journal-article","created":{"date-parts":[[2020,10,30]],"date-time":"2020-10-30T11:18:39Z","timestamp":1604056719000},"page":"277-281","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Effect of\n Rhodiola sachalinensis<\/i>\n Aqueous Extract on\n In Vitro<\/i>\n Maturation of Porcine Oocytes and Subsequent\n In Vitro<\/i>\n Embryonic Development"],"prefix":"10.1177","volume":"22","author":[{"given":"Caifeng","family":"Wu","sequence":"first","affiliation":[{"name":"Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agriculture Sciences, Shanghai, China."},{"name":"Division of Animal Genetic Engineering, Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Shanghai, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Defu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agriculture Sciences, Shanghai, China."},{"name":"Division of Animal Genetic Engineering, Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Shanghai, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shushan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agriculture Sciences, Shanghai, China."},{"name":"Division of Animal Genetic Engineering, Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Shanghai, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingwei","family":"Sun","sequence":"additional","affiliation":[{"name":"Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agriculture Sciences, Shanghai, China."},{"name":"Division of Animal Genetic Engineering, Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Shanghai, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Animal, Dairy, Veterinary Sciences, Utah State University, Logan, Utah, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianjun","family":"Dai","sequence":"additional","affiliation":[{"name":"Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agriculture Sciences, Shanghai, China."},{"name":"Division of Animal Genetic Engineering, Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Shanghai, China."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2020,12,2]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:BEBM.0000020211.24779.15"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2013-6623"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.4161\/epi.6.2.13519"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2010.01.043"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2015-9761"},{"key":"e_1_3_3_7_1","first-page":"63","article-title":"Reactive oxygen species generation and use of antioxidants during In vitro maturation of oocytes","volume":"11","author":"Khazaei M.","year":"2017","unstructured":"Khazaei M., , and Aghaz F. 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